Startup O2k-Respirometer
| Description | OROBOROS O2k-Core - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Core includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Core supports all add-on O2k-Modules of the O2k, and is a component of the O2k-Fluorometer.
On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.001 °C in the range of 2 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software. The O2k is a sole source apparatus with no other instruments meeting its specifications. |
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| Product ID | 10000-02 |
| Type | O2k, O2k-Module, O2k-Fluorometer, Catalogue |
| Link | O2k, @OROBOROS, Gnaiger_2008_POS |
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MitoPedia O2k and high-resolution respirometry:
O2k hardware
The O2k-Core[1] does not include, but fully supports and is required for the add-on Modules of the O2k, such as the TIP2k, O2k-Fluo LED2-Module, O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module.
- ↑ A PC is required (not included), connecting DatLab to the O2k via a USB port. Minimum requirement: Pentium III, 256 MB, Win 2000™ or Win XP™.
The O2k-Core consists of
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O2k-Core Manual and selected protocols
Contents: O2k-Core Manual DatLab 6.pdf
| Chapter | Section | Last update |
|---|---|---|
| Gnaiger 2008 POS | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | 2008 |
| Gnaiger 2020 BEC MitoPathways | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
| MiPNet04.05 Titration-Injection |  | 1999-01 |
| MiPNet07.08 User information |  | 2017-11-03 |
| MiPNet08.09 CellRespiration | | 2016-08-11 |
| MiPNet08.12 IOC22 | | 2011-12-11 |
| MiPNet12.01 Suppl T-issue | | 2018-11-18 |
| MiPNet12.11 MitoRespiration | | 2014-02-20 |
| MiPNet17.03 Shredder vs Fibers | | 2015-06-18 |
| MiPNet19.18 O2k-Series G Core manual | | 2016-08-08 |
| MiPNet19.18B POS-service | | 2021-06-23 |
| MiPNet19.18C DatLab 6: Guide | | 2016-08-24 |
| MiPNet19.18D O2k-Series G and DatLab 6: Calibration | | 2016-08-24 |
| MiPNet19.18E O2 flux analysis - DatLab 6 | | 2016-08-08 |
| MiPNet21.14 Reference sample HRR | | 2016-10-19 |
| MiPNet21.16 DatLab 7 Innovations |  | 2019-09-23 |
| MiPNet22.16 DatLab 7 Innovations: DL-Protocols | | 2019-09-13 |
| MiPNet23.12 DL-Protocols help | | 2019-09-13 |
| MiPNet24.06 Oxygen flux analysis - DatLab 7.4 | | 2020-04-16 |
| MiPNet24.08 Safranin Analysis Template | | 2020-05-13 |
| MiPNet24.09 Data analysis of mt-membrane potential | | 2021-09-16 |
| MiPNet25.14 TPP Analysis Template | | 2020-##-## |
| MiPNet26.06 DatLab 7: Guide | | 2021-06-02 |
| MiPNet28.10 SmartPOS-service | | 2023-09-29 |
| Pesta 2012 Methods Mol Biol | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | 2012 |
