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This page provides a simple browsing interface for finding entities described by a property and a named value. Other available search interfaces include the page property search, and the ask query builder.

List of results

Rhodamine 123 + (Rhodamine 123 (Rh123) is an [[extrinsic fluorophores|extrinsic fluorophore]] … Rhodamine 123 (Rh123) is an [[extrinsic fluorophores|extrinsic fluorophore]] and can be used as a probe to determine changes in [[Mitochondrial_membrane_potential|mitochondrial membrane potential]]. Rh123 is a lipophilic cation that is accumulated by mitochondria in proportion to Δ''ψ''mt. Using ethanol as the solvent, the excitation maximum is 511 nm and the emission maximum is 534 nm. The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively (Sigma-Aldrich). are 488 and 515-575 nm, respectively (Sigma-Aldrich).)
Bioblasts + (Richard Altmann (1894) defined the 'elemen … Richard Altmann (1894) defined the 'elementary organisms' as '''Bioblasts'''. He observed granula in cells stained with osmium and viewed ‘the protoplasm as a colony of bioblasts’. "Microorganisms and granula are at an equivalent level and represent elementary organisms, which are found wherever living forces are acting, thus we want to describe them by the common term bioblasts. In the bioblast, that morphological unit of living matter appears to be found." [[Altmann 1894 Verlag Von Veit & Comp|Altmann 1894]]; p. 141. Altmann is thus considered as the discoverer of [[mitochondria]] (the granula), which constitute together with the microorganisms the ''bioblasts'' (the elementary organisms). Bioblasts are the aliens with permanent residence in our cells ([[Bioblasts#Bioblasts_.E2.80.93_the_aliens_with_permanent_residence_in_our_cells|Gnaiger 2010]]).oblasts#Bioblasts_.E2.80.93_the_aliens_with_permanent_residence_in_our_cells|Gnaiger 2010]]).)
Zenodo + (Science Europe: "Zenodo is an open source … Science Europe: "Zenodo is an open source and free repository for storing data, code, materials, and any research artefact. It was created by CERN and launched within the frame of the OpenAIRE project, commissioned by the European Commission. It aims at fostering free and easy access to scientific results, scientific data, software, and publications to all researchers."are, and publications to all researchers.")
ASAPbio + (Science only progresses as quickly and eff … Science only progresses as quickly and efficiently as it is shared. But even with all of the technological capabilities available today, the process of publishing scientific work is taking longer than ever. '''ASAPbio''' (Accelerating Science and Publication in biology) is a scientist-driven nonprofit working to address this problem by promoting innovation and transparency in life sciences communication.In 2015, ASAPbio founder Ron Vale published an analysis of the increasing time to first-author publication among graduate students at UCSF, and proposed a more widespread use of preprints in the life sciences as a potential solution.the life sciences as a potential solution.)
Substrate control state + (See '''[[Electron-transfer-pathway state]]''')
ET-pathway substrate types + (See '''[[Electron-transfer-pathway state]]''')
Physiological pathway-control state + (See [[Electron-transfer-pathway state]].)
Fluorescent marker + (See [[Extrinsic fluorophores]])
Delete points + (Select '''Delete points''' in the [[Marks - DatLab |Mark information]] window to remove all data points in the marked section of the active plot. See also [[Interpolate points]] and [[Restore points]] or [[Recalculate slope]].)
Interpolate points + (Select '''Interpolate points''' in the [[Marks - DatLab |Mark information]] window to interpolate all data points in the marked section of the active graph. See also [[Delete points]] and [[Restore points]] or [[Recalculate slope]].)
Mouse control: Zoom + (Select '''Mouse Control: Zoom''' in the Graph-menu or press [Ctrl+Z].)
Recalculate slope + (Select '''Recalculate slope''' (Recalc. sl … Select '''Recalculate slope''' (Recalc. slope) in the [[Marks - DatLab |Mark information]] window to restore data points in the marked section of the active Flux / Slope plot, if [[Delete points]] or [[Interpolate points]] was used before. The entire plot is recalculated, such that other marked sections which may have been deleted are also restored. Compare [[Restore points]].[[Restore points]].)
Restore points + (Select '''Restore points''' in the [[Marks - DatLab |Mark information]] window to restore data points in the marked section of the active signal plot, if [[Delete points]] or [[Interpolate points]] was used before. Compare [[Recalculate slope]].)
Manage setups and templates - DatLab + (Setups and templates in DatLab can be renamed or deleted under '''Manage setups''' or '''Manage templates'''.)
Graph options - DatLab + (Several display options can be applied to a DatLab graph under '''Graph options'''.)
Comma for separating a term and its abbreviation + (Should we used a '''comma for separating a … Should we used a '''comma for separating a term and its abbreviation''' in the text? The SI Brochure frequently does not use a comma. The comma might be added, if it helps to clarify the distinction between the term and its abbreviation. The example “reduced Q fraction, ''Q''r” – the sequence of Q and ''Q''r may be confusing without comma. There will always be examples, where it is not clear, if a comma is needed.l always be examples, where it is not clear, if a comma is needed.)
Multicomponent analysis + (Similarly to the [[least squares method]] … Similarly to the [[least squares method]], '''multicomponent analysis''' makes use of all of the data points of the spectrum in order to analyse the concentration of the component parts of a measured spectrum. To do this, two or more reference spectra are combined using iterative statistical techniques in order to achieve the best fit with the measured spectrum.e the best fit with the measured spectrum.)
Holode + (Small entetic units are counted into the reference system on a balance opposite to the experimental system with the large sample, which in balance contains as many abstract units as the count of entetic units in the reference system.)
Sodium phosphate buffer + (Sodium phosphate buffer, '''Na-PB''', for [[HRR]] with freeze-dried baker´s yeast.)
Spline + (Some [[spectrofluorometer]] … Some [[spectrofluorometer]] or [[spectrophotometer]] software offers the possibility of '''spline''' interpolation of the spectral data points. This makes use of a polynomial (the number of '''spline''' points is entered by the user) to interpolate the curve between the data points.rpolate the curve between the data points.)
Mitochondrial density + (Specific '''mitochondrial density''' is '' … Specific '''mitochondrial density''' is ''DmtE'' = ''mtE''·''mX''-1 [mtEU·kg-1]. If the amount of mitochondria, ''mtE'', is expressed as mitochondrial mass, then ''DmtE'' is the mass fraction of mitochondria in the sample. If ''mtE'' is expressed as mitochondrial volume, ''V''mt, and the mass of sample, ''mX'', is replaced by volume of sample, ''VX'', then ''DmtE'' is the volume fraction of mitochondria in the sample.eplaced by volume of sample, ''VX'', then ''DmtE'' is the volume fraction of mitochondria in the sample.)
Resolution + (Spectral resolution is a measure of the ab … Spectral resolution is a measure of the ability of an instrument to differentiate between two adjacent wavelengths. Two wavelengths are normally considered to be resolved if the minimum detector output signal (trough) between the two peaks is lower than 80 % of the maximum. The resolution of a [[spectrofluorometer]] or [[spectrophotometer]] is dependent on its [[bandwidth]].[[bandwidth]].)
Custom-made stoppers + (Stoppers can be custom-made for applications with user-specific sensors according to customer specifications.)
Tartronic acid + (Tartronic acid (hydroxymalonic acid, C3H4O5; molecular weight 120.06) is an inhibitor of [[malic enzyme]].)
Taurine + (Taurine, or 2-Aminoethan sulfonic acid, is … Taurine, or 2-Aminoethan sulfonic acid, is one of the most abundant low-molecular-weight organic constituents in animals and humans. It has a multitude of functions in different types of tissue, one of which is the stabilization of membranes. Because of this and its antioxidative effect, taurine is a component of the respiration media MiR05 and MiR06 to preserve mitochondrial function. MiR06 to preserve mitochondrial function.)
Chinese Society of Mitochondrial Research and Medicine + (The '''Chinese Society of Mitochondrial Research and Medicine''' (Chinese-Mit) is a member of [[Asian Society for Mitochondrial Research and Medicine|ASMRM]].)
Crabtree effect + (The '''Crabtree effect''' describes the ob … The '''Crabtree effect''' describes the observation that respiration is frequently inhibited when high concentrations of glucose or fructose are added to the culture medium - a phenomenon observed in numerous cell types, particularly in proliferating cells, not only tumor cells but also bacteria and yeast. The Pasteur effect (suppression of glycolysis by oxygen) is the converse of the Crabtree effect (suppression of respiration by high concentration of glucose or fructose).igh concentration of glucose or fructose).)
Default label + (The '''Default label''' is the system default value for the axis label. These labels are changed automatically, according to the selected channel and unit. To change this label enter a [[Custom label]].)
Directory of Open Access Journals + (The '''Directory of Open Access Journals''' is a free online directory that indexes and provides access to open access peer-reviewed journals.)
Exclusion criteria + (The '''Exclusion criteria''' include factors or characteristics that make the recruited population ineligible for the outcome parameter. With the [[Inclusion criteria]], this factor must be a cofounder for the outcome parameter)
Faraday constant + (The '''Faraday constant''' ''F'' links the … The '''Faraday constant''' ''F'' links the electric charge [C] to amount [mol], and thus relates the [[electrical format]] ''e'' [C] to the [[molar format]] ''n'' [mol]. The Farady constant, ''F'' = ''e''·''N''A = 96 485.33 C/mol, is the product of [[elementary charge]], ''e'' = 1.602176634∙10-19 C/x, and the [[Avogadro constant]], ''N''A = 6.02214076∙1023 x/mol. The dimensionless unit [x] is not explicitely considered by IUPAC.= 6.02214076∙1023 x/mol. The dimensionless unit [x] is not explicitely considered by IUPAC.)
Inclusion criteria + (The '''Inclusion criteria''' are based on … The '''Inclusion criteria''' are based on key features of the target population that the researchers will use to answer their question. These criteria should identify the study population in a consistent, reliable, uniform, and objective manner. With the [[Exclusion criteria]], this factor must be a cofounder for the outcome parametert be a cofounder for the outcome parameter)
International Standard Serial Number + (The '''International Standard Serial Numbe … The '''International Standard Serial Number''', ISSN, is a code used to identify periodical publications, independent of which media are used (print and/or electronic). - [[Bioenergetics Communications]], BEC: [https://portal.issn.org/resource/ISSN/2791-4690 ISSN 2791-4690]rg/resource/ISSN/2791-4690 ISSN 2791-4690])
International System of Units + (The '''International System of Units''' (S … The '''International System of Units''' (SI) is the modern form of the metric system of [[unit]]s for use in all aspects of life, including international trade, manufacturing, security, health and safety, protection of the environment, and in the basic science that underpins all of these. The system of quantities underlying the SI and the equations relating them are based on the present description of nature and are familiar to all scientists, technologists and engineers. The definition of the SI units is established in terms of a set of seven defining constants. The complete system of units can be derived from the fixed values of these defining constants, expressed in the units of the SI. These seven defining constants are the most fundamental feature of the definition of the entire system of units. These particular constants were chosen after having been identified as being the best choice, taking into account the previous definition of the SI, which was based on seven base units, and progress in science (p. 125).e units, and progress in science (p. 125).)
International Union of Pure and Applied Chemistry, IUPAC + (The '''International Union of Pure and App … The '''International Union of Pure and Applied Chemistry''' (IUPAC) celebrated in 2019 the 100th anniversary, which coincided with the [https://iupac.org/united-nations-proclaims-international-year-periodic-table-chemical-elements/ International Year of the Periodic Table of Chemical Elements (IYPT 2019)]. IUPAC {''Quote''} notes that marking Mendeleev's achievement will show how the periodic table is central to connecting cultural, economic, and political dimensions of global society “through a common language” {''end of Quote''} (Horton 2019). 2019 is proclaimed as the [https://iupac.org/united-nations-proclaims-international-year-periodic-table-chemical-elements/ International Year of the Periodic Table of Chemical Elements (IYPT 2019)]. For a '''common language''' in mitochondrial physiology and bioenergetics, the IUPAC ''Green book'' (Cohen et al 2008) is a most valuable resource, which unfortunately is largely neglected in bioenergetics textbooks. Integration of [[ergodynamics |open systems and non-equilibrium thermodynamic]] approaches remains a challenge for developing a common language (Gnaiger 1993; [[BEC 2020.1]]).C 2020.1]]).)
Korean Society of Mitochondrial Research and Medicine + (The '''Korean Society of Mitochondrial Research and Medicine''' (KSMRM) is a member of [[Asian Society for Mitochondrial Research and Medicine|ASMRM]].)
MitoFit DOI Data Center + (The '''MitoFit DOI Data Center''' is respo … The '''MitoFit DOI Data Center''' is responsible for the provision of digital identifiers, for the storage and ensuring the persistence of the scientific objects, the provision of access, review process and maintenance of the Metadata, and quality control.ance of the Metadata, and quality control.)
Mitochondrial Physiology Network + (The '''Mitochondrial Physiology Network''' is the on-line Oroboros journal.)
N/NS pathway control ratio + (The '''N/NS [[pathway control ratio]] … The '''N/NS [[pathway control ratio]]''' is obtained when succinate is added to N-linked respiration in a defined coupling state. N and NS are abbreviations for respiration in the [[N-pathway control state]] (with pyruvate, glutamate, malate, or other ETS competent N-linked substrate combinations) and the [[NS-pathway control state]] (N in combination with succinate). NS indicates respiration with a cocktail of substrates supporting the N- and S-pathways.bstrates supporting the N- and S-pathways.)
N/S pathway control ratio + (The '''N/S [[pathway control ratio]] … The '''N/S [[pathway control ratio]]''' is obtained from SUIT protocols when the [[N-pathway control state |N-pathway flux]] and [[S-pathway control state |S-pathway flux]] are measured in the same [[coupling control state]]. The N/S pathway control ratio may be larger or smaller than 1.0, depending on the mitochondrial source and various mitochondrial injuries. The S-pathway control state may be selected preferentially as reference state, if mitochondria are studied with respect to N-pathway injuries.tudied with respect to N-pathway injuries.)
NS-N pathway control efficiency + (The '''NS-N [[pathway control efficiency]]''', ''j''NS-N = 1-N/NS, expresses the fractional change of flux when succinate is added to the [[N-pathway control state]] in a defined [[coupling-control state]].)
NS-S pathway control efficiency + (The '''NS-S pathway control efficiency''' … The '''NS-S pathway control efficiency''' expresses the relative stimulation of succinate supported respiration (S) by NADH-linked substrates (N), with the [[S-pathway control state]] as the [[background state]] and the [[NS-pathway control state]] as the [[reference state]]. In typical [[SUIT protocol]]s with [[Electron-transfer-pathway state |type N and S substrates]], flux in the [[NS-pathway control state]] NS is inhibited by [[rotenone]] to measure flux in the [[S-pathway control state]], S(Rot) or S. Then the NS-S pathway control efficiency in the ET-coupling state is ''j''(NS-S)''E'' = (NS''E''-S''E'')/NS''E''The NS-S pathway control efficiency expresses the fractional change of flux in a defined [[coupling-control state]] when inhibition by [[rotenone]] is removed from flux under S-pathway control in the presence of a type N substrate combination. Experimentally rotenone Rot is added to the NS-state. The reversed protocol, adding N-substrates to a S-pathway control background does not provide a valid estimation of S-respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] CII.[[succinate dehydrogenase]] CII.)
O2k signal line + (The '''O2k signal line''' is underneath th … The '''O2k signal line''' is underneath the [[O2k status line]]. It shows, depending on the [[O2k series]], on the left side the O2k number, the time of the experiment, the oxygen raw signal of each chamber, the [[block temperature]], the [[barometric pressure]], the Peltier power, the recorded amperometric and potentiometric raw signal, the enviromental (room) temperature and the signal from internal sensors recording the humidity and temperature of the electronics. On the right side of the O2k signal line the current [[User code - DatLab|user]], the DatLab version and the [[O2k serial number]] are displayed.[[O2k serial number]] are displayed.)
O2k-Accessory Box + (The '''O2k-Accessory Box''' contains components of the [[POS-Service Kit]] and the [[O2k-Assembly Kit]] and is shipped with the O2k.)
O2k-Assembly Kit + (The '''O2k-Assembly Kit''' is a component … The '''O2k-Assembly Kit''' is a component of the [[Oroboros O2k]], consisting of 2 [[Stirrer-Bar\white PVDF\15x6 mm|PVDF Stirrer-Bars]], 2 [[PEEK]] O2k-Stoppers, [[OroboPOS-Connector]]s for O2k-series A-I and NextGen-O2k series XA (attached to the [[O2k-Main Unit]]) and cables (power supply, USB-connection). Several components of the O2k-Assembly Kit are included in the [[O2k-Accessory Box]] either for shipment or for storage.[[O2k-Accessory Box]] either for shipment or for storage.)
O2k-Fluo Smart-Module + (The '''O2k-Fluo Smart-Module''' is an ampe … The '''O2k-Fluo Smart-Module''' is an amperometric add-on module to the [[Startup_O2k-Respirometer| O2k-Respirometer]], adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex® UltraRed), ATP production (Magnesium Green™), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium Green™), and numerous other applications open for O2k-user innovation. ::: » [[MiPNet28.09 O2k-Fluo Smart-Module manual]]et28.09 O2k-Fluo Smart-Module manual]])
O2k-Main Basic + (The '''O2k-Main Basic''' is an integral el … The '''O2k-Main Basic''' is an integral element of the [[O2k-Main Unit]]. The Oroboros O2k Main Basic has the following components:*Stainless-Steel Housing*Switching power supply*Microprocessor for integrated control, A/D converters and data handling*Copper-Block with windows to 2 O2k-Chambers*2 Amperometric OroboPOS Plugs*TIP2k socket, providing the basis for add-on of the [[TIP2k]]*2 Potentiometric Plugs for ion sensitive electrodes (ISE: TPP+, Ca2+; pH), providing the basis for add-on of the [[O2k-MultiSensor]] Modules*2 Amperometric Plugs, providing the basis for add-on of the [[O2k-Fluo LED2-Module]] or NO (H2S) sensors.*USB-Port for connection with DatLab (PC or laptop not included)for connection with DatLab (PC or laptop not included))
O2k-Main Unit + (The '''O2k-Main Unit''' is a component of … The '''O2k-Main Unit''' is a component of the [[O2k-Core]]. The O2k-Main Unit consists of functionally defined, integral elements, the ([[O2k-Main Basic]], [[O2k-Peltier Temperature Control]], two [[O2k-Electromagnetic Stirrer Twin-Control]] units, two [[O2k-Amperometric OroboPOS Twin-Channel]]s, [[O2k-Barometric Pressure Transducer]]), which cannot be obtained separately.[[O2k-Barometric Pressure Transducer]]), which cannot be obtained separately.)
O2k chamber volume calibration + (The '''O2k-chamber volume calibration''' has to be done before getting started with the [[Oroboros O2k]] to guarantee a standard [[chamber volume]] of 2.0 mL.)
O2k-ticket system + (The '''O2k-ticket system''' is a customer … The '''O2k-ticket system''' is a customer support platform based on Zammad. This system automatically attributes an unique Ticket number (which is visible on the subject of your e-mail) to each received customer inquiry. For an easy follow-up, all the related correspondence is collected under this Ticket number. * Contact us: '''[email protected]'''In order to provide a helpful and reliable support regarding your O2k/equipment, we suggest to include in your inquiries:* your affiliation and your O2k-serial number - ''See'': [[O2k_series]]* DLD file(s) with your reported issue accompanied by a brief explanation. issue accompanied by a brief explanation.)
Oxygen sensor test + (The '''O2 sensor te … The '''O2 sensor test''' is an important component of [[MitoPedia: Oroboros QM |Oroboros Quality Management]]. The [[OroboPOS]] test is described in detail in [[MiPNet06.03 POS-calibration-SOP]], is performed after switching on the [[Oroboros O2k]], and is required as a basis of technical service of the instrument.red as a basis of technical service of the instrument.)
OXPHOS International + (The '''OXPHOS International''' web portal is a repository of information useful to scholars studying mitochondria. The site is operated as a private "special interests" community hub.)
Oroboros USB-flash drive + (The '''Oroboros USB-flash drive''' is deli … The '''Oroboros USB-flash drive''' is delivered with the [[Oroboros O2k]]. Copy the folder "Oroboros O2k-Course on HRR" from the '''Oroboros USB-flash drive''' to your computer. This folder contains the DatLab installation program as well as tools to find topics, O2k-manuals and O2k-protocols with corresponding DatLab demo files and templates for training with [[DatLab]].[[DatLab]].)
Q-redox state + (The '''Q-redox state''' reflects the redox … The '''Q-redox state''' reflects the redox status of the [[Q-junction]] in the mitochondrial or chloroplast [[ETS|electron transfer system (ETS)]]. [[Coenzyme Q]] (CoQ or Q, [[ubiquinone]]) is a mobile redox component located centrally in the mitochondrial [[ETS]], while plastoquinones are essential mobile components in the photosynthetic system with a similar function. The Q-redox state depends on the balance between reducing capacities of convergent electron entries from fuel substrates into the Q-junction and oxidative capacities downstream of Q to the electron acceptor oxygen. Therefore, deficiencies in the mitochondrial [[ETS]], originating from e.g. the malfunction of respiratory Complexes, can be detected by measuring the changes of the Q-redox state with respect to the respiratory activity.A three-electrode system was implemented into the NextGen-O2k to monitor the Q-redox state continuously and simultaneously with respiratory oxygen consumption. Added CoQ2 reflects the mitochondrial Q-redox state when equilibrating both with the detecting electrode and the biological sites (e.g. Complexes I, II and III).ical sites (e.g. Complexes I, II and III).)
S/NS pathway control ratio + (The '''S/NS [[pathway control ratio]] … The '''S/NS [[pathway control ratio]]''' is obtained when [[rotenone]] (Rot) is added to the [[NS-pathway control state]] in a defined [[coupling control state]]. The reversed protocol, adding N-type substrates to a [[S-pathway control state]] as the [[background state]] does not provide a valid estimation of S-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII).[[succinate dehydrogenase]] (CII).)
SUIT protocol pattern + (The '''SUIT protocol pattern''' describes the type of the sequence of coupling and substrate control steps in a SUIT protocol, which may be liner, orthogonal, or diametral.)
Science Citation Index + (The '''Science Citation Index''' SCI offers bibliographical access to a curated collection of journals across 178 scientific disciplines. The SCI provides gold-standard lists of established journals.)
SUIT protocol library + (The '''Substrate-uncoupler-inhibitor titra … The '''Substrate-uncoupler-inhibitor titration (SUIT) protocol library''' contains a sequential list of SUIT protocols (D001, D002, ..) with links to the specific SUIT pages. Classes of [[SUIT|SUIT protocols]] are explained with coupling and substrate control defined for [[mitochondrial preparations]].[[mitochondrial preparations]].)
Taiwan Society for Mitochondrial Research and Medicine + (The '''Taiwan Society for Mitochondrial Research and Medicine''' (TSMRM) is a member of [[Asian Society for Mitochondrial Research and Medicine|ASMRM]].)
USB port + (The '''USB port''' describes the connection between O2k and Computer. With the USB cable connected, select '''USB port''' in the [[Connection window]]. Depending on the O2k series, it is possible to connect with a '''USB port''' or [[Serial port]].)
Abscissa + (The '''abscissa''' is the horizontal axis … The '''abscissa''' is the horizontal axis ''x'' of a rectangular two-dimensional graph with the [[ordinate]] ''y'' as the vertical axis. Values ''X'' are placed horizontally from the origin.See [[Abscissal X/Y regression |Abscissal ''X''/''Y'' regression]].[[Abscissal X/Y regression |Abscissal ''X''/''Y'' regression]].)
Accuracy + (The '''accuracy''' of a method is the degree of agreement between an individual test result generated by the method and the true value.)
Activity + (The '''activity''' (relative activity) is … The '''activity''' (relative activity) is a dimensionless quantity related to the concentration or partial pressure of a dissolved substance. The activity of a dissolved substance B equals the [[concentration]], ''c''B [mol·L-1], at high dilution divided by the unit concentration, ''c''° = 1 mol·L-1: ''a''B = ''c''B/''c''°This simple relationship applies frequently to substances at high dilutions <10 mmol·L-1 (<10 mol·m-3). In general, the concentration of a [[solute]] has to be corrected for the activity coefficient (concentration basis), ''γ''B, ''a''B = ''γ''B·''c''B/''c''°At high dilution, ''γ''B = 1. In general, the relative activity is defined by the [[chemical potential]], ''µ''B ''a''B = exp[(''µ''B-''µ''°)/''RT'']potential]], ''µ''B ''a''B = exp[(''µ''B-''µ''°)/''RT''])
Adenine nucleotide translocase + (The '''adenine nucleotide translocator''', … The '''adenine nucleotide translocator''', ANT, exchanges [[ADP]] for [[ATP]] in an electrogenic antiport across the inner mt-membrane. The ANT is inhibited by [[atractyloside]], [[carboxyatractyloside|carboxyatractyloside]] and [[bongkrekik acid]]. The ANT is a component of the [[phosphorylation system]].[[phosphorylation system]].)
Advantage of preprints + (The '''advantages of preprints''', the excitement and concerns about the role that preprints can play in disseminating research findings in the life sciences are discussed by N Bhalla (2016).)
Aerobic + (The '''aerobic''' state of metabolism is d … The '''aerobic''' state of metabolism is defined by the presence of oxygen (air) and therefore the potential for oxidative reactions (ox) to proceed, particularly in [[oxidative phosphorylation]] (OXPHOS). Aerobic metabolism (with involvement of oxygen) is contrasted with [[anaerobic]] metabolism (without involvement of oxygen): Whereas anaerobic ''metabolism'' may proceed in the absence or presence of oxygen (anoxic or oxic ''conditions''), aerobic ''metabolism'' is restricted to oxic ''conditions''. Below the [[critical oxygen pressure]], aerobic ATP production decreases.[[critical oxygen pressure]], aerobic ATP production decreases.)
Amount of substance + (The '''amount of substance''' ''n'' is a b … The '''amount of substance''' ''n'' is a base physical quantity, and the corresponding SI unit is the [[mole]] [mol]. Amount of substance (sometimes abbreviated as 'amount' or 'chemical amount') is proportional to the number ''N''''X'' of specified elementary entities ''X'', and the universal proportionality constant is the reciprocal value of the [[Avogadro constant]] ([[Bureau International des Poids et Mesures_2019_The International System of Units (SI) |SI]]), ''n''''X'' = ''N''''X''·''N''A-1''n''''X'' contained in a system can change due to internal and external transformations, d''n''''X'' = di''n''''X'' + de''n''''X''In the absence of nuclear reactions, the amount of any atom is conserved, ''e.g.'', for carbon di''n''C = 0. This is different for chemical substances or ionic species which are produced or consumed during the [[advancement]] of a reaction r, :::: [[File:Amount dn.png|100px]]A change in the amount of ''X''''i'', d''n''''i'', in an open system is due to both the internal formation in chemical transformations, dr''n''''i'', and the external transfer, de''n''''i'', across the system boundaries. d''n''''i'' is positive if ''X''''i'' is formed as a product of the reaction within the system. de''n''''i'' is negative if ''X''''i'' flows out of the system and appears as a product in the surroundings ([[Cohen 2008 IUPAC Green Book]]).[Cohen 2008 IUPAC Green Book]]).)
Amplitude + (The '''amplitude''' of the [[absorbance spectrum]] … The '''amplitude''' of the [[absorbance spectrum]] can be described in terms of the [[absorbance]] differences between the characteristic peaks (absorbance maxima) and troughs (absorbance minima) (see [[absorbance spectrum]]) for substances present in the sample.[[absorbance spectrum]]) for substances present in the sample.)
Background state + (The '''background state''' Y (background r … The '''background state''' Y (background rate ''YX'') is the non-activated or inhibited respiratory state at background rate, which is low in relation to the higher rate ''ZX'' in the [[reference state]] Z. The transition from the background state to the reference state is a step change. A [[metabolic control variable]] ''X'' (substrate, activator) is added to the background state to stimulate flux to the level of the reference state. Alternatively, the metabolic control variable ''X'' is an inhibitor, which is present in the background state Y, but absent in the reference state Z. The background state is the baseline of a single step in the definition of the [[flux control efficiency]]. In a sequence of step changes, the common [[baseline state]] is the state of lowest flux in relation to all steps, which can be used as a [[baseline correction]].[[baseline correction]].)
Baseline state + (The '''baseline state''' in a sequence of … The '''baseline state''' in a sequence of step changes is the state of lowest flux in relation to all steps, which can be used as a [[baseline correction]]. Correction for [[residual oxygen consumption]], ROX, is an example where ROX is the baseline state. In a single step, the baseline state is equivalent to the [[background state]].[[background state]].)
Bias + (The '''bias''' is defined as the difference between the mean of the measurements and the reference value. In general, the measuring instrument calibration procedures should focus on establishing and correcting it.)
Block temperature + (The '''block temperature''' of the [[Oroboros O2k]] is the continuously measured temperature of the copper block, housing the two glass chambers of the O2k. The block temperature is recorded by [[DatLab]] as one of the O2k system channels.)
Body mass excess + (The '''body mass excess''', BME, is an ind … The '''body mass excess''', BME, is an index of obesity and as such BME is a lifestyle metric. The BME is a measure of the extent to which your actual [[body mass]], ''M'' [kg/x], deviates from ''M''° [kg/x], which is the reference body mass [kg] per individual [x] without excess body fat in the [[healthy reference population]], HRP. A balanced BME is BME° = 0.0 with a band width of -0.1 towards underweight and +0.2 towards overweight. The BME is linearly related to the [[body fat excess]].body fat excess]].)
Body mass index + (The '''body mass index''', BMI, is the rat … The '''body mass index''', BMI, is the ratio of body mass to height squared (BMI=''M''·''H''-2), recommended by the WHO as a general indicator of underweight (BMI<18.5 kg·m-2), overweight (BMI>25 kg·m-2) and obesity (BMI>30 kg·m-2). Keys et al (1972; see 2014) emphasized that 'the prime criterion must be the relative independence of the index from height'. It is exactly the dependence of the BMI on height - from children to adults, women to men, Caucasians to Asians -, which requires adjustments of BMI-cutoff points. This deficiency is resolved by the [[body mass excess]] relative to the [[healthy reference population]].althy reference population]].)
Body mass + (The '''body mass''' ''M'' is the mass ([[kilogram]] … The '''body mass''' ''M'' is the mass ([[kilogram]] [kg]) of an individual (object) [x] and is expressed in units [kg/x]. Whereas the body weight changes as a function of gravitational force (you are weightless at zero gravity; your floating weight in water is different from your weight in air), your mass is independent of gravitational force, and it is the same in air and water.orce, and it is the same in air and water.)
Bound energy + (The '''bound energy''' change in a closed … The '''bound energy''' change in a closed system is that part of the ''total'' [[energy]] change that is always bound to an exchange of [[heat]], d''B'' = d''U'' - d''A'' [Eq. 1] ∆''B'' = ∆''H'' - ∆''G'' [Eq. 2]The ''free'' energy change (Helmoltz or Gibbs; d''A'' or d''G'') is the ''total'' energy change (total inner energy or enthalpy, d''U'' or d''H'') of a system minus the ''bound'' energy change.Therefore, if a process occurs at [[equilibrium]], when d''G'' = 0 (at constant gas pressure), then d''H'' = d''B'', and at de''W'' = 0 (d''H'' = de''Q'' + de''W''; see [[energy]]) we obtain the definition of the bound energy as the heat change taking place in an equilibrium process (eq), d''B'' = ''T''∙d''S'' = de''Q''eq [Eq. 3]rocess (eq), d''B'' = ''T''∙d''S'' = de''Q''eq [Eq. 3])
Cell count and normalization in HRR + (The '''cell count''' ''N''ce< … The '''cell count''' ''N''ce is the number of cells, expressed in the abstract [[unit]] [x] (1 Mx = 106 x). The ''elementary entity'' cell ''U''ce [x] is the real unit, the 'single individual cell'. A cell count is the multitude or number ''N'' of cells, ''N''ce = ''N''·''U''ce ([[Gnaiger MitoFit Preprints 2020.4]]). Normalization of respiratory rate by cell count yields oxygen [[flow]] ''I''O2 expressed in units [amol·s-1·x-1] (=10-18 mol·s-1·x-1).gt; expressed in units [amol·s-1·x-1] (=10-18 mol·s-1·x-1).)
Chamber volume + (The '''chamber volume''' of the O2k is 2.0 … The '''chamber volume''' of the O2k is 2.0 mL or 0.5 mL of aqueous medium with or without sample, excluding the volume of the stirrer and the volume of the capillary of the stopper (see: [[Cell count and normalization in HRR]]). A modular extension of the O2k, the [[O2k-sV-Module]], was specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all components necessary for the smaller operation volume of 0.5 mL.or the smaller operation volume of 0.5 mL.)
Charge number + (The '''charge number''' of an ion ''X'' or … The '''charge number''' of an ion ''X'' or electrochemical reaction with unit stoichiometric number of ''X'' is the [[particle charge]] [C·x-1] divided by the [[elementary charge]] [C·x-1]. The particle charge ''Q''''N''''X'' is the charge per count of ions ''X'' or per ion ''X'' transferred in the reaction as defined in the reaction equation.ns ''X'' or per ion ''X'' transferred in the reaction as defined in the reaction equation.)
Chemical potential + (The '''chemical potential''' of a substanc … The '''chemical potential''' of a substance B, ''µ''B [J/mol], is the partial derivative of Gibbs energy, ''G'' [J], per amount of B, ''n''B [mol], at constant temperature, pressure, and composition other than that of B, ''µ''B = (∂''G''/∂''n''B)''T'',''p'',''nj''≠BThe chemical potential of a [[solute]] in solution is the sum of the standard chemical potential under defined standard conditions and a concentration ([[activity]])-dependent term, ''µ''B = ''µ''B° + ''RT'' ln(''a''B)The standard state for the solute is refered to ideal behaviour at standard concentration, ''c''° = 1 mol/L, exhibiting infinitely diluted solution behaviour [1]. ''µ''B° equals the standard molar Gibbs energy of formation, Δf''G''B° [kJ·mol-1]. The formation process of B is the transformation of the pure constituent elements to one mole of substance B, with all substances in their standard state (the most stable form of the element at 100 kPa (1 bar) at the specified temperature) [2].on of the pure constituent elements to one mole of substance B, with all substances in their standard state (the most stable form of the element at 100 kPa (1 bar) at the specified temperature) [2].)
Comparison of respirometric methods + (The '''comparison of respirometric methods''' provides the basis to evaluate different instrumental platforms and different [[mitochondrial preparations]], as a guide to select the best approach and to critically evaluate published results.)
Critical oxygen pressure + (The '''critical oxygen pressure''', ''p''& … The '''critical oxygen pressure''', ''p''c, is defined as the partial oxygen pressure, ''p''O2, below which [[aerobic]] catabolism (respiration or oxygen consumption) declines significantly. If [[anaerobic]] catabolism is activated simultaneously to compensate for lower aerobic ATP generation, then the '''[[limiting oxygen pressure]]''', ''p''l, is equal to the ''p''c. In many cases, however, the ''p''l is substantially lower than the ''p''c.y cases, however, the ''p''l is substantially lower than the ''p''c.)
Cytochrome c control efficiency + (The '''cytochrome ''c'' control efficiency … The '''cytochrome ''c'' control efficiency''' expresses the control of respiration by externally added [[cytochrome c | cytochrome ''c'']], c, as a fractional change of flux from substrate state CHNO to CHNOc. These fluxes are corrected for ''Rox'' and may be measured in the OXPHOS state or ET state, but not in the LEAK state. In this [[flux control efficiency]], CHNOc is the [[reference state]] with stimulated flux; CHNO is the [[background state]] with CHNO substrates, upon which c is added: ''j''cyt ''c'' = (''J''CHNOc-''J''CHNO)/''J''CHNOc.>CHNOc-''J''CHNO)/''J''CHNOc.)
Data recording interval + (The '''data recording interval''' is the t … The '''data recording interval''' is the time interval at which data is sampled with an instrument. In [[DatLab]] the data recording interval is set in the [[O2k control]] window. The system default value is 2 s. A lower data recording interval is selected for kinetic experiments, and when the volume-specific oxygen flux is high (>300 pmol O2·s-1·ml-1). Technically, the O2k instrument (hardware) measures the sensor signal every 10ms (which is NOT the „data recording interval“). By the given data recording interval from DatLab (software) a discrete number of sensor signal points are taken to calculate an average value in the O2k (e.g. a data recording interval of 2 s can take 200 sensor signal points; a data recording interval of 0.5 s can take 50 sensor signal points). This average value is sent to DatLab and is recorded as a raw data point. However, there is a defined threshold: the O2k does not apply more than 200 sensor signal points to calculate the average for the raw data point. For example a data recording interval of 3 s could take 300 sensor signal points but only the 200 most recent sensor signal points are used for averaging.signal points but only the 200 most recent sensor signal points are used for averaging.)
Dicarboxylate carrier + (The '''dicarboxylate carrier''' is a transporter which catalyses the electroneutral exchange of [[malate]]2- (or [[succinate]]2-) for inorganic [[phosphate]], HPO42-.)
Energy charge + (The '''energy charge''' of the adenylate s … The '''energy charge''' of the adenylate system or adenylate energy charge (AEC) has been defined by Atkinson and Walton (1967) as (ATP + ½ ADP)/(AMP + ADP + ATP). Wheather the AEC is a fundamental metabolic control parameter remains a controversial topic.l parameter remains a controversial topic.)
Ergodynamic efficiency + (The '''ergodynamic efficiency''', ''ε'' (c … The '''ergodynamic efficiency''', ''ε'' (compare [[thermodynamic efficiency]]), is a power ratio between the output power and the (negative) input power of an energetically coupled process. Since [[power]] [W] is the product of a [[flow]] and the conjugated thermodynamic [[force]], the ergodynamic efficiency is the product of an output/input flow ratio and the corresponding force ratio. The efficiency is 0.0 in a fully uncoupled system (zero output flow) or at level flow (zero output force). The maximum efficiency of 1.0 can be reached only in a fully (mechanistically) coupled system at the limit of zero flow at ergodynamic equilibrium. The ergodynamic efficiency of coupling between ATP production (DT phosphorylation) and oxygen consumption is the flux ratio of DT phosphorylation flux and oxygen flux (P»/O2 ratio) multiplied by the corresponding force ratio. Compare with the [[OXPHOS-coupling efficiency]].OXPHOS-coupling efficiency]].)
Extinction coefficient + (The '''extinction coefficient''' (''ε'') of a substance is the [[absorbance]] of a 1 µmolar concentration over a 1 cm pathlength and is wavelength-dependent.)
Gain + (The '''gain''' is an amplification factor applied to an input signal to increase the output signal.)
Glutamate-aspartate carrier + (The '''glutamate-aspartate carrier''' cata … The '''glutamate-aspartate carrier''' catalyzes the electrogenic antiport of glutamate- +H+ for aspartate-. It is an important component of the malate-aspartate shuttle in many mitochondria. Due to the symport of glutamate- + +H+, the glutamate-aspartate antiport is not electroneutal and may be impaired by [[uncoupling]]. [[Aminooxyacetate]] is an [[inhibitor]] of the glutamate-aspartate carrier.[[inhibitor]] of the glutamate-aspartate carrier.)
Height of humans + (The '''height of humans''', ''h'', is give … The '''height of humans''', ''h'', is given in SI units in meters [m]. Humans are countable objects, and the symbol and unit of the number of objects is ''N'' [x]. The average height of ''N'' objects is, ''H'' = ''h''/''N'' [m/x], where ''h'' is the heights of all ''N'' objects measured on top of each other. Therefore, the height per human has the unit [m·x-1] (compare [[body mass]] [kg·x-1]). Without further identifyer, ''H'' is considered as the standing height of a human, measured without shoes, hair ornaments and heavy outer garments., measured without shoes, hair ornaments and heavy outer garments.)
Hexokinase + (The '''hexokinase''' catalyzes the phosphorylation of D-glucose at position 6 by ATP to yield D-glucose 6-phosphate as well as the phosphorylation of many other hexoses like D-fructose, D-mannose, D-glucosamine.)
Limiting oxygen pressure + (The '''limiting oxygen pressure''', ''p''& … The '''limiting oxygen pressure''', ''p''l, is defined as the partial oxygen pressure, ''p''O2, below which [[anaerobic]] catabolism is activated to contribute to total ATP generation. The limiting oxygen pressure, ''p''l, may be substantially lower than the '''[[critical oxygen pressure]]''', ''p''c, below which [[aerobic]] catabolism (respiration or oxygen consumption) declines significantly.[[aerobic]] catabolism (respiration or oxygen consumption) declines significantly.)
Membrane-bound ET pathway + (The '''membrane-bound [[electron transfer pathway]] … The '''membrane-bound [[electron transfer pathway]] (mET pathway)''' consists in mitochondria mainly of [[respiratory complexes]] CI, CII, electron transferring flavoprotein complex (CETF), glycerophosphate dehydrogenase complex (CGpDH), and choline dehydrogenase, with [[convergent electron flow]] at the [[Q-junction]] (Coenzyme Q), and the two downstream respiratory complexes connected by cytochrome ''c'', CIII and CIV, with oxygen as the final electron acceptor. The mET-pathway is the terminal (downstream) module of the mitochondrial [[ET pathway]] and can be isolated from the ET-pathway in [[submitochondrial particles]] (SmtP).[[submitochondrial particles]] (SmtP).)
Meter + (The '''meter''', symbol m, is the SI unit … The '''meter''', symbol m, is the SI unit of the SI base quantity [[length]] ''l''. It is defined by taking the fixed numerical value of the speed of light ''c'' in vacuum to be 299 792 458 when expressed in the unit m·s−1, where the second is defined in terms of the caesium frequency Δ''ν''Cs.in terms of the caesium frequency Δ''ν''Cs.)
Mitochondrial ATP-sensitive K+ channel + (The '''mitochondrial ATP-sensitive K+ channel''' (mtKATP or mitoKATP).)
Mitochondrial free radical theory of aging + (The '''mitochondrial free radical theory o … The '''mitochondrial free radical theory of aging''' goes back to Harman (1956) and ranks among the most popular theories of aging. It is based on postulates which are not unequivocally supported by observation (Bratic, Larsson 2013):(i) Mitochondrial ROS production increases with age caused by progressive mitochondrial dysfunction;(ii) antioxidat capacity declines with age;(iii) mutations of somatic mtDNA accumulate during aging;(iv) a vicious cycle occurs of increased ROS production caused by mtDNA mutations and degenerated mt-function, and due to ROS-induced ROS production.on, and due to ROS-induced ROS production.)
Mitochondrial inner membrane + (The '''mitochondrial inner membrane''' mtI … The '''mitochondrial inner membrane''' mtIM is the structure harboring the membrane-bound [[electron transfer system]] ETS including the respiratory complexes working as [[hydrogen ion pump]]s, the mt-[[phosphorylation system]] including the hydrogen ion pump [[ATP synthase]], several substrate transporters involved in the [[electron transfer pathway]], and a variety of other ion pumps that carry [[proton]] charge (Ca2+, Mg2+). The [[protonmotive force]] is the electrochemical potential difference across the mtIM generated by the [[hydrogen ion pumps]] of the .[[hydrogen ion pumps]] of the .)
Mitochondrial matrix + (The '''mitochondrial matrix''' (mt-matrix) … The '''mitochondrial matrix''' (mt-matrix) is enclosed by the mt-inner membrane mtIM. The terms mitochondrial matrix space or mitochondrial lumen are used synonymously. The mt-matrix contains the enzymes of the [[tricarboxylic acid cycle]], [[fatty acid oxidation]] and a variety of enzymes that have cytosolic counterparts (e.g. [[glutamate dehydrogenase]], [[malic enzyme]]). Metabolite concentrations, such as the concentrations of fuel substrates, adenylates (ATP, ADP, AMP) and redox systems (NADH), can be very different in the mt-matrix, the mt-intermembrane space, and the cytosol. The finestructure of the gel-like mt-matrix is subject of current research. mt-matrix is subject of current research.)
Mitochondrial membrane potential + (The '''mitochondrial membrane potential''' … The '''mitochondrial membrane potential''' difference, mtMP or Δ''Ψ''p+ = Δel''F''''e''p+, is the electric part of the protonmotive [[force]], Δp = Δm''F''''e''H+.:::: Δel''F''''e''p+ = Δm''F''''e''H+ - Δd''F''''e''H+:::: Δ''Ψ''p+ = Δp - Δ''µ''H+·(''z''H+·''F'')-1Δ''Ψ''p+ is the potential difference across the mitochondrial inner membrane (mtIM), expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per ‘motive’ charge or per charge moved across the transmembrane potential difference, with the number of ‘motive’ charges expressed in the unit coulomb [C].t;p+ is the potential difference across the mitochondrial inner membrane (mtIM), expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per ‘motive’ charge or per charge moved across the transmembrane potential difference, with the number of ‘motive’ charges expressed in the unit coulomb [C].)
Mitochondrial outer membrane + (The '''mitochondrial outer membrane''' is … The '''mitochondrial outer membrane''' is the incapsulating membrane which is osmotically not active and contains the cytochrome ''b''5 enzyme similar to that found in the endoplasmatic reticulum, the translocases of the outer membrane, monoaminooxidase, the palmitoyl-CoA synthetase and carnytil-CoA transferase 1.lmitoyl-CoA synthetase and carnytil-CoA transferase 1.)
Motive unit + (The '''motive unit''' [MU] is the variable … The '''motive unit''' [MU] is the variable SI unit in which the [[motive entity]] (transformant) of a transformation is expressed, which depends on the energy transformation under study and on the chosen [[format]]. Fundamental MU for electrochemical transformations are:* MU = x, for the particle or molecular format, ''N''* MU = mol, for the chemical or molar format, ''n''* MU = C, for the electrical format, ''e''; For the [[protonmotive force]] the motive entity is the proton with charge number ''z''=1. The protonmotive force is expressed in the electrical or molar format with MU J/C=V or J/mol=Jol, respectively. The conjugated flows, ''I'', are expressed in corresponding electrical or molar formats, C/s = A or mol/s, respectively.The [[charge number]], ''z'', has to be considered in the conversion of motive units (compare Table below), if a change not only of units but a transition between the entity [[elementary charge]] and an entity with charge number different from unity is involved (''e.g.'', O2 with ''z''=4 in a redox reaction). The ratio of elementary charges per reacting O2 molecule (''z''O2=4) is multiplied by the elementary charge (''e'', coulombs per proton), which yields coulombs per O2 [C∙x-1]. This in turn is multiplied with the [[Avogadro constant]], ''N''A (O2 molecules per mole O2 [x∙mol-1]), thus obtaining for ''zeN''A the ratio of elementary charges [C] per amount of O2 [mol-1]. The conversion factor for O2 is 385.94132 C∙mmol-1., thus obtaining for ''zeN''A the ratio of elementary charges [C] per amount of O2 [mol-1]. The conversion factor for O2 is 385.94132 C∙mmol-1.)
Ordinate + (The '''ordinate''' is the vertical axis ''y'' of a rectangular two-dimensional graph with the [[abscissa]] ''x'' as the horizontal axis. Values ''Y'' are placed vertically from the origin. See [[Ordinary Y/X regression |Ordinary ''Y''/''X'' regression]].)
Oxycaloric equivalent + (The '''oxycaloric equivalent''' is the the … The '''oxycaloric equivalent''' is the theoretically derived enthalpy change of the oxidative catabolic reactions per amount of oxygen respired, Deltak''H''O2, ranging from -430 to -480 kJ/mol O2. The oxycaloric equivalent is used in [[indirect calorimetry]] to calculate the theoretically expected metabolic heat flux from the respirometrically measured metabolic oxygen flux. [[Calorespirometric ratio|Calorimetric/respirometric ratios]] (CR ratios; heat/oxygen flux ratios) are experimentally determined by [[calorespirometry]]. A CR ratio more exothermic than the oxycaloric equivalent of -480 kJ/mol indicates the simultaneous involvement of aerobic and anaerobic mechanisms of energy metabolism.ltaneous involvement of aerobic and anaerobic mechanisms of energy metabolism.)
Oxygen signal + (The '''oxygen signal''' of the [[Oroboros O2k]] … The '''oxygen signal''' of the [[Oroboros O2k]] is transmitted from the electrochemical polarographic oxygen sensor ([[OroboPOS]]) for each of the two O2k-chambers to [[DatLab]]. The primary signal is a current [µA] which is converted into a voltage [V] (raw signal), and calibrated in SI units for amount of substance concentration [µmol·L-1 or µM]. For technical reasons, the raw signal is given in [V] (DatLab 7 and previous) or [µA] (DatLab 8). The value of the raw signal is the same, independent of the displayed unit ([V] or [µA]). In the following sections, only [µA] is used for information on the raw signal, but the same values in [V] apply for the raw signal when using DL7 or previous versions.or the raw signal when using DL7 or previous versions.)
Oxygen solubility factor + (The '''oxygen solubility factor''' of the … The '''oxygen solubility factor''' of the incubation medium, ''F''M, expresses the effect of the salt concentration on [[oxygen solubility]] relative to pure water. In mitochondrial respiration medium [[MiR05]], [[MiR05-Kit]] and [[MiR06]], ''F''M is 0.92 (determined at 30 and 37 °C) and in culture media is 0.89 (at 37 °C). ''F''M varies depending on the temperature and composition of the medium. To determine the FM based on the oxygen concentration, specific methods and equipment are needed (see references Rasmussen HN, Rasmussen UF 2003 in [https://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP MiPNet06.03]). For other media, ''F''M may be estimated using Table 4 in [https://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP MiPNet06.03]. For this purpose KCl based media can be described as "seawater" of varying salinity. The original data on sucrose and KCl-media (Reynafarje et al 1985), however, have been critizesed as artefacts and the ''F''M of 0.92 is suggested in the temperature range of 10 °C to 40 °C as for MiR05.M of 0.92 is suggested in the temperature range of 10 °C to 40 °C as for MiR05.)
Oxygen solubility + (The '''oxygen solubility''', ''S''<sub& … The '''oxygen solubility''', ''S''O2 [µM/kPa] = [(µmol·L-1)/kPa], expresses the oxygen concentration in solution in equilibrium with the [[oxygen pressure]] in a gas phase, as a function of temperature and composition of the solution. The inverse of oxygen solubility is related to the [[activity]] of dissolved oxygen. The oxygen solubility in solution, ''S''O2(aq), depends on temperature and the concentrations of solutes in solution, whereas the dissolved oxygen concentration at equilibrium with air, ''c''O2*(aq), depends on ''S''O2(aq), barometric pressure and temperature. ''S''O2(aq) in pure water is 10.56 µM/kPa at 37 °C and 12.56 µM/kPa at 25 °C. At standard [[barometric pressure]] (100 kPa), ''c''O2*(aq) is 207.3 µM at 37 °C (19.6 kPa partial oxygen pressure) or 254.7 µM at 25 °C (20.3 kPa partial oxygen pressure). In [[MiR05]] and serum, the corresponding saturation concentrations are lower due to the [[oxygen solubility factor]]: 191 and 184 µM at 37 °C or 234 and 227 µM at 25 °C.lubility factor]]: 191 and 184 µM at 37 °C or 234 and 227 µM at 25 °C.)
PH + (The '''pH value''' or pH is the negative o … The '''pH value''' or pH is the negative of the base 10 logarithm of the [[activity]] of [[proton]]s (hydrogen ions, H+). A [[pH electrode]] reports the pH and is sensitive to the activity of H+. In dilute solutions, the hydrogen ion activity is approximately equal to the hydrogen ion [[concentration]]. The symbol pH stems from the term ''potentia hydrogenii''.[[concentration]]. The symbol pH stems from the term ''potentia hydrogenii''.)
Partial oxygen pressure + (The '''partial oxygen pressure''' ''p''< … The '''partial oxygen pressure''' ''p''O2 [kPa] is the contribution of the O2 gas pressure to the total gas pressure. According to the gas law, the partial oxygen pressure is ''p''O2(g) = ''n''O2(g)·''V''·''RT'', where the [[concentration]] is ''c''O2(g) = ''n''O2(g)·''V''-1 [mol·m-3], ''R'' is the [[gas constant]], and ''T'' is the absolute temperature, and ''RT'' is expressed in units of chemical force [J·mol-1]. In aqueous solutions at equilibrium with a gas phase, the partial O2 pressures are equal in the aqueous phase (aq) and gas phase (g), ''p''O2(aq) = ''p''O2(g) at total [[pressure]]s where the partial pressure equals the fugacity. The O2 concentration in the aqueous phase, however, is much lower than in the gas phase, due to the low [[oxygen solubility]] in water. The activity of dissolved O2 is expressed by the ''p''O2, where the [[solubility]] can be seen as an activity coefficient.ubility]] can be seen as an activity coefficient.)
Particle charge + (The '''particle charge''' ''QN& … The '''particle charge''' ''QNX'' (''QNX'') or charge per elementary entity is the [[charge]] ''Q''el''X'' [C] carried by ions of type ''X'' divided by the count ''NX'' [x]. The particle charge per proton is the [[elementary charge]] or proton charge ''e''.[[elementary charge]] or proton charge ''e''.)
Pascal + (The '''pascal''' [Pa] is the SI unit for [[pressure]] … The '''pascal''' [Pa] is the SI unit for [[pressure]]. [Pa] = [J·m-3] = [N·m-2] = [m-1·kg·s-2].The standard pressure is 100 kPa = 1 bar (105 Pa; 1 kPa = 1000 Pa). Prior to 1982 the standard pressure has been defined as 101.325 kPa or 1 standard atmosphere (1 atm = 760 mmHg).982 the standard pressure has been defined as 101.325 kPa or 1 standard atmosphere (1 atm = 760 mmHg).)
Phosphate carrier + (The '''phosphate carrier''' (PiC) is a pro … The '''phosphate carrier''' (PiC) is a proton/phosphate symporter which transports negatively charged [[inorganic phosphate]] across the inner mt-membrane. The transport can be described either as symport of H+ with Pi, or antiport of hydroxide anion against Pi. The phosphate carrier is a component of the [[phosphorylation system]].[[phosphorylation system]].)
Primary sample + (The '''primary sample''' or '''specimen''' … The '''primary sample''' or '''specimen''' is a set of one or more parts initially taken from an object. In some countries, the term “specimen” is used instead of primary sample (or a subsample of it), which is the sample prepared for sending to, or as received by, the laboratory and which is intended for examination.ory and which is intended for examination.)
Protonmotive force + (The '''protonmotive force''' ∆m … The '''protonmotive force''' ∆m''F''H+ is known as Δp in Peter Mitchell’s chemiosmotic theory [1], which establishes the link between electric and chemical components of energy transformation and coupling in [[oxidative phosphorylation]]. The unifying concept of the ''pmF'' ranks among the most fundamental theories in biology. As such, it provides the framework for developing a consistent theory and nomenclature for mitochondrial physiology and bioenergetics. The protonmotive force is not a vector force as defined in physics. This conflict is resolved by the generalized formulation of isomorphic, compartmental [[force]]s, ∆tr''F'', in energy (exergy) transformations [2]. Protonmotive means that there is a potential for the movement of protons, and force is a measure of the potential for motion.The ''pmF'' is generated in [[oxidative phosphorylation]] by oxidation of reduced fuel substrates and reduction of O2 to H2O, driving the coupled proton translocation from the mt-matrix space across the mitochondrial inner membrane (mtIM) through the proton pumps of the [[electron transfer pathway]] (ETS), which are known as respiratory Complexes CI, CIII and CIV. ∆m''F''H+ consists of two partial isomorphic forces: (''1'') The chemical part, ∆d''F''H+, relates to the diffusion (d) of uncharged particles and contains the chemical potential difference§ in H+, ∆''µ''H+, which is proportional to the pH difference, ∆pH. (''2'') The electric part, ∆el''F''p+ (corresponding numerically to ∆''Ψ'')§, is the electric potential difference§, which is not specific for H+ and can, therefore, be measured by the distribution of any permeable cation equilibrating between the negative (matrix) and positive (external) compartment. Motion is relative and not absolute (Principle of Galilean Relativity); likewise there is no absolute potential, but isomorphic forces are stoichiometric potential differences§.The total motive force (motive = electric + chemical) is distinguished from the partial components by subscript ‘m’, ∆m''F''H+. Reading this symbol by starting with the proton, it can be seen as ''pmF'', or the subscript m (motive) can be remembered by the name of Mitchell, ∆m''F''H+ = ∆d''F''H+ + ∆el''F''p+With classical symbols, this equation contains the [[Faraday constant]], ''F'', multiplied implicitly by the charge number of the proton (''z''H+ = 1), and has the form [1] ∆p = ∆''µ''H+∙''F''-1 + ∆''Ψ''A partial electric force of 0.2 V in the electrical [[format]], ∆el''F''''e''H+''a'', is 19 kJ∙mol-1 H+''a'' in the molar format, ∆el''F''''n''p+''a''. For 1 unit of ∆pH, the partial chemical force changes by -5.9 kJ∙mol-1 in the molar format, ∆d''F''''n''H+''a'', and by 0.06 V in the electrical format, ∆d''F''''e''H+''a''. Considering a driving force of -470 kJ∙mol-1 O2 for oxidation, the thermodynamic limit of the H+''a''/O2 ratio is reached at a value of 470/19 = 24, compared to the mechanistic stoichiometry of 20 for the [[N-pathway]] with three coupling sites.)
Protonmotive pressure + (The '''protonmotive pressure''', ∆<sub& … The '''protonmotive pressure''', ∆m''Π''H+ or ''pmP'' [kPa], is an extension of Peter Mitchell’s concept of the [[protonmotive force]] ''pmF'', based on Fick’s law of diffusion and Einstein’s diffusion equation, accounting for osmotic pressure (corresponding to the diffusion term in the ''pmF'') and electric pressure (the electric term or membrane potential in the ''pmF''). The linearity of the generalized flow-pressure relationship explains the non-ohmic flow-force dependence in the proton leak rate as a function of membrane potential.The total motive pressure (motive = electric + chemical) is distinguished from the partial components by subscript ‘m’, ∆m''Π''H+, ∆m''Π''H+ = ∆d''Π''H+ + ∆el''Π''p+ub>''Π''H+ = ∆d''Π''H+ + ∆el''Π''p+)
Raw signal of the oxygen sensor + (The '''raw signal''' of the polarographic … The '''raw signal''' of the polarographic oxygen sensor is the [[current]] ''I''el [µA], 1 µA = 10-6 C·s-1, (DatLab 8) or the electric potential difference ([[voltage]]) [V], 1 V = 1 J·C-1, obtained after a current-to-voltage conversion in the O2k (DatLab 7 and previous versions).btained after a current-to-voltage conversion in the O2k (DatLab 7 and previous versions).)
Reference state + (The '''reference state''' Z (reference rat … The '''reference state''' Z (reference rate ''ZX'') is the respiratory state with high flux in relation to the [[background state]] Y with low background flux ''YX''. The transition between the background state and the reference state is a step brought about by a [[metabolic control variable]] ''X''. If ''X'' stimulates flux (ADP, fuel substrate), it is present in the reference state but absent in the background state. If ''X'' is an inhibitor of flux, it is absent in the reference state but present in the background state. The reference state is specific for a single step to define the [[flux control efficiency]]. In contrast, in a sequence of multiple steps, the common reference state is frequently taken as the state with the highest flux in the entire sequence, as used in the definition of the [[flux control ratio]].[[flux control ratio]].)
Respiratory acceptor control ratio + (The '''respiratory acceptor control ratio' … The '''respiratory acceptor control ratio''' (''RCR'') is defined as [[State 3]]/[[State 4]] [1]. If State 3 is measured at saturating [ADP], ''RCR'' is the inverse of the OXPHOS control ratio, ''[[L/P]]'' (when State 3 is equivalent to the OXPHOS state, ''P''). ''RCR'' is directly but non-linearly related to the [[P-L control efficiency |''P-L'' control efficiency]], ''j''''P-L'' = 1-''L/P'', with boundaries from 0.0 to 1.0. In contrast, ''RCR'' ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses. In living cells, the term ''RCR'' has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''[[L/E]]'' ratio [2,3]. Then for conceptual and statistical reasons, ''RCR'' should be replaced by the [[E-L coupling efficiency |''E-L'' coupling efficiency]], 1-''L/E'' [4].[[E-L coupling efficiency |''E-L'' coupling efficiency]], 1-''L/E'' [4].)
Signal-to-noise ratio + (The '''signal to noise ratio''' is the ratio of the power of the signal to that of the noise. For example, in [[fluorimetry]] it would be the ratio of the square of the [[fluorescence]] intensity to the square of the intensity of the background noise.)
Slit width + (The '''slit width''' determines the amount of light entering the [[spectrofluorometer]] or [[spectrophotometer]]. A larger slit reduces the [[signal-to-noise ratio]] but reduces the wavelength [[resolution]].)
Solubility + (The '''solubility''' of a gas, ''S''G, is defined as concentration divided by partial pressure, ''S''G = ''c''G·''p''G-1.)
SUIT reference protocol + (The '''substrate-uncoupler-inhibitor titra … The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[SUIT-001]] - RP1 and [[SUIT-002]] - RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.: [[SUIT-001]] (RP1): 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd: [[SUIT-002]] (RP2): 1D;2OctM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11AzdtM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd)
Mitochondrial transcription factor A + (The '''transcription factor A''' is a gene … The '''transcription factor A''' is a gene that encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. TFAM is downstream of [[Peroxisome proliferator-activated receptor gamma coactivator 1-alpha|PGC-1alpha]].[[Peroxisome proliferator-activated receptor gamma coactivator 1-alpha|PGC-1alpha]].)
Tricarboxylate carrier + (The '''tricarboxylate carrier''' in the inner mt-membrane exchanges malate2- for citrate3- or isocitrate3-, with co-transport of H+.)
Tricarboxylic acid cycle + (The '''tricarboxylic acid (TCA) cycle''' i … The '''tricarboxylic acid (TCA) cycle''' is a system of enzymes in the mitochondrial matrix arranged in a cyclic metabolic structure, including dehydrogenases that converge in the NADH pool and [[succinate dehydrogenase]] (on the inner side of the inner mt-membrane) for entry into the membrane-bound ET pathway [[Membrane-bound ET pathway|mET pathway]]. [[Citrate synthase]] is a marker enzyme of the TCA cycle, at the gateway into the cycle from [[pyruvate]] via [[acetyl-CoA]]. It is thus the major module of the [[Electron transfer pathway]], upstream of the inner [[Membrane-bound ET pathway|Membrane-bound ET pathway]] (mET-pathway) and downstream of the [[Mitochondrial outer membrane|outer mt-membrane]]. Sections of TCA cycle are required for [[fatty acid oxidation]] (FAO, β-oxidation). [[Anaplerosis|Anaplerotic reactions]] fuel the TCA cycle with other intermediary metabolites. In the cell, the TCA cycle serves also biosynthetic functions by metabolite export from the matrix into the cytosol.e export from the matrix into the cytosol.)
Uncoupling-control ratio + (The '''uncoupling-control ratio''' UCR is … The '''uncoupling-control ratio''' UCR is the ratio of ET-pathway/ROUTINE-respiration (''E/R'') in living cells, evaluated by careful [[uncoupler]] titrations ([[Steinlechner-Maran 1996 Am J Physiol Cell Physiol|Steinlechner et al 1996]]). Compare [[ROUTINE-control ratio]] (''R/E'') [[Gnaiger 2008 POS|(Gnaiger 2008)]].[[Gnaiger 2008 POS|(Gnaiger 2008)]].)
Journal volume + (The '''volume''' of a journal or periodica … The '''volume''' of a journal or periodical is a number, which in many cases indicates the sequential number of years the journal has been published. Alternatively, the volume number may indicate the current year, independent of the year in which the journal published its first volume. A volume may be subdivided into [[Journal issue |issues]].[[Journal issue |issues]].)
Wet mass + (The '''wet mass''' of a tissue or biological sample, obtained after blotting the sample to remove an arbitrary amount of water adhering externally to the sample.)
Permeability transition pore + (The (mitochondrial, mt) permeability trans … The (mitochondrial, mt) permeability transition pore (PTP) is an unspecific pore presumed to involve components of both the inner and outer mt membrane which upon opening induces a massive increase of the inner mt membrane permeability for solutes up to 1.5 kDa. It is crucially involved in cell death induction in response to, among other stimuli, radical stress and/or calcium overload and may cause necrosis or apoptosis. It plays an important role in neurodegenerative diseases, cardiac ischemia-reperfusion injury and possibly various other diseases. Previously considered essential molecular constituents such as the voltage-dependent anion channel (VDAC), the adenine nucleotide translocator (ANT) and cyclophilin D (CypD) have all been shown to be important regulators of mtPTP opening, but the molecular entities actually forming the pore are still unknown at present. The opening of the pore can be prevented using [[cyclosporin A]], a compound that binds cyclophilin D avoiding the formation of the pore. In respirometry, mtPTP opening may be observed as a sudden decrease of respiration of isolated mitochondria ([[Hansson 2010 J Biol Chem]]).[[Hansson 2010 J Biol Chem]]).)
Search for defective O2k components + (The 2-chamber design of the O2k helps to '''search for defective O2k components''', by switching components linked to O2k chambers A and B between sides A and B.)
P»-system + (The ADP-ATP phosphorylation system or P»-system. ''See'' [[Phosphorylation system]].)
CDGSH iron-sulfur domain proteins + (The CDGSH iron-sulfur domain (CISDs) famil … The CDGSH iron-sulfur domain (CISDs) family of proteins uniquely ligate labile 2Fe-2S clusters with a 3Cys-1His motif. CISD1 and CISD3 have been demonstrated to localize to the outer mitochondrial membrane and mitochondrial matrix respectively, however their relationship to mitochondrial physiology remains ill-defined [1]. The best characterized member of the CISD family, CISD1, has been demonstrated to be involved in respiratory capacity, iron homeostasis, and ROS regulationcity, iron homeostasis, and ROS regulation)
French Group of Bioenergetics + (The French Group of Bioenergetics...)
O2k control panel - DatLab + (The O2k control panel allows for quick acc … The O2k control panel allows for quick access of O2k instrument settings. It covers the right side of the graphical user interface of DatLab 8. If a DatLab protocol is active, the protocol panel ist shown instead, a tab at the right side allows to switch between O2k control panel and protocol panel.ween O2k control panel and protocol panel.)
Closed chamber + (The O2k-chamber can be used as a [[closed system]] or [[open system]]. Gas bubbles must be avoided.)
OroboPOS-Connector Service + (The OroboPOS-Connector Service entails routine maintenance and any necessary repairs of the OroboPOS-Connector in the Oroboros electronics workshop (WGT).)
PC requirements + (The PC requirements for controlling an O2k and data recording with [[DatLab]] are found [[DatLab installation |here]].)
Display Power-O2k + (The Power-O2k number, which is set in the … The Power-O2k number, which is set in the pull-down menu Oroboros O2k \ [[O2k configuration]], is shown in the active graph. To show it in graphs copied to clipboard, the option "Show Oroboros icon in clipboard files" must be enabled in the Graph-menu [[Graph options - DatLab]].[[Graph options - DatLab]].)
TIP2k - DatLab + (The Titration-Injection microPump (TIP2k) provides automated injection of liquids into both O2k chambers. It is controlled via DatLab, allowing for programmable titration regimes and feedback control.)
MtOM + (The [[Mitochondrial outer membrane| '''mitochondrial outer membrane''']])
Succinate transport + (The [[dicarboxylate carrier]] catalyses the electroneutral exchange of succinate2- for HPO4-2-.)
Ampere + (The ampere, symbol A, is the SI unit of el … The ampere, symbol A, is the SI unit of electric current. It is defined by taking the fixed numerical value of the elementary charge ''e'' to be 1.602 176 634 × 10−19 when expressed in the unit C, which is equal to A s, where the second is defined in terms of Δ''ν''Cs.the second is defined in terms of Δ''ν''Cs.)
Isolated system + (The boundaries of '''isolated system'''s a … The boundaries of '''isolated system'''s are impermeable for all forms of energy and matter. Changes of isolated systems have exclusively internal origins, ''e.g.'', internal entropy production, di''S''/d''t'', internal formation of chemical species ''i'' which is produced in a reaction ''r'', di''ni''/d''t'' = dr''ni''/d''t''. In isolated systems some internal terms are restricted to zero by various conservation laws which rule out the production or destruction of the respective quantity. by various conservation laws which rule out the production or destruction of the respective quantity.)
Calorespirometric ratio + (The calorimetric/respirometric or '''calor … The calorimetric/respirometric or '''calorespirometric ratio''' (CR ratio) is the ratio of calorimetrically and respirometrically measured heat and oxygen flux, determinded by [[calorespirometry]]. The experimental CR ratio is compared with the theoretically derived [[oxycaloric equivalent]], and agreement in the range of -450 to -480 kJ/mol O2 indicates a balanced [[aerobic]] energy budget ([[Gnaiger_1987_PhysiolZool|Gnaiger and Staudigl 1987]]). In the transition from aerobic to [[anaerobic | anaerobic metabolism]], there is a [[Limiting pO2|limiting ''p''O2]], ''p''lim, below which CR ratios become more exothermic since anaerobic energy flux is switched on.h CR ratios become more exothermic since anaerobic energy flux is switched on.)
Candela + (The candela, symbol cd, is the SI unit of … The candela, symbol cd, is the SI unit of luminous intensity in a given direction. It is defined by taking the fixed numerical value of the luminous efficacy of monochromatic radiation of frequency 540 × 1012 Hz, ''K''cd, to be 683 when expressed in the unit lm W−1.;/sub>, to be 683 when expressed in the unit lm W−1.)
Illumination + (The chambers of the [[OROBOROS O2k|Oroboros O2k]] … The chambers of the [[OROBOROS O2k|Oroboros O2k]] are illuminated by an internal LED. The '''illumination''' is switched on and off in [[DatLab]] during the experiment by pressing [F10]. This illumination must be distinguished from light introduced into the chambers by LEDs for the purpose of spectrophotometric and fluorometric measurements. For these, the internal illumination must be switched off.nternal illumination must be switched off.)
Matrix-ETS + (The component of the electron transfer sys … The component of the electron transfer system located in the mitochondrial matrix ('''matrix-ETS''') is distringuished from the ETS bound to the mt-inner membrane (membrane-ETS). Electron transfer and corresponding OXPHOS capacities are classically studied in mitochondrial preparations as oxygen consumption supported by various fuel substrates undergoing partial oxidation in the mt-matrix, such as pyruvate, malate, succinate, and others.s pyruvate, malate, succinate, and others.)
Affinity of reaction + (The concept of '''affinity''' and hence ch … The concept of '''affinity''' and hence chemical force is deeply rooted in the notion of '''attraction''' (and repulsion) of alchemy, which was the foundation of chemistry originally, but diverted away from laboratory experiments towards occult secret societies [1].** Newton's extensive experimental alchemical work and his substantial written track record on alchemy (which he did not publish) is seen today as a key inspiration for his development of the concept of the gravitational force [2-4]. This marks a transition of the meaning of affinity, from the descriptive 'adjacent' (proximity) to the causative 'attractive' (force) [5]. Correspondingly, Lavoisier (1790) equates affinity and force [6]: “''... the degree of force or affinity with which the acid adheres to the base''” [5]. By discussing the influence of electricity and gravity on chemical affinity, Liebig (1844) considers affinity as a force [7]. This leads to Guldberg and Waage's [[mass action ratio]] ('Studies concerning affinity', 1864; see [5]), the free energy and chemical affinity of Helmholtz (1882 [8]), and chemical thermodynamics of irreversible processes [9], where flux-force relations are center stage [10]. According to the IUPAC definition, the '''affinity of reaction''', ''A'' [J·mol-1], equals the negative molar Gibbs energy of reaction [11], which is the negative Gibbs [[force]] of reaction (derivative of [[Gibbs energy]] per [[advancement]] of reaction [12]): -''A'' = Δr''F'' = ∂''G''/∂r''ξ''The historical account of affinity is summarized by concluding, that today affinity of reaction should be considered as an isomorphic motive '''force''' and be generalized as such. This will help to (''1'') avoid confusing reversals of sign conventions (repulsion = negative attraction; pull = negative push), (''2'') unify symbols across classical and nonequilibrium thermodynamics [12,13], and thus (''3'') facilitate interdisciplinary communication by freeing ourselves from the alchemical, arcane scientific nomenclature.ry communication by freeing ourselves from the alchemical, arcane scientific nomenclature.)
Latent mitochondrial dysfunction + (The concept on '''latent mitochondrial dys … The concept on '''latent mitochondrial dysfunction''' presents the working hypothesis that the dynamic mitochondrial stress response provides a more sensitive and integrative marker for degenerative disease-related defects compared to acute mitochondrial dysfunction. The risk for developing a disease may be quantified in terms of a stress response, rather than a static pathophysiological state. Acute and latent mitochondrial dysfunction are studied at baseline and in response to a particular (e.g. oxidative) stress, using a mitochondrial stress resistance test.ng a mitochondrial stress resistance test.)
Mark specifications - DatLab + (The function '''Mark specifications''' is … The function '''Mark specifications''' is largely replaced by [[SUIT: Browse DL-Protocols and templates |SUIT DL-Protocols]] and [[Instrumental: Browse DL-Protocols and templates |Instrumental DL-Protocols]] in [https://www.oroboros.at/index.php/product/datlab/ DatLab 7.4]. Mark specifications allow the user to rename [[Marks - DatLab| Marks]] in the active plot and save/recall the settings. Rename marks individually by clicking into the horizontal bar, or use corresponding templates for renaming the entire sequence of marks.for renaming the entire sequence of marks.)
Hydride + (The hydride anion is the species H−.)
Illumination on/off + (The illumination in both chambers is switched on/off.)
Kelvin + (The kelvin, symbol K, is the SI unit of thermodynamic temperature. It is defined by taking the fixed numerical value of the Boltzmann constant ''k'' to be 1.380 649 × 10−23 when expressed in the unit J x-1 K−1.)
Kilogram + (The kilogram, symbol kg, is the SI unit of … The kilogram, symbol kg, is the SI unit of mass. It is defined by taking the fixed numerical value of the Planck constant ''h'' to be 6.626 070 15 × 10−34 when expressed in the unit J s, which is equal to kg m2 s−1, where the meter and the second are defined in terms of ''c'' and Δ''ν''Cs.he meter and the second are defined in terms of ''c'' and Δ''ν''Cs.)
Malate-aspartate shuttle + (The malate-aspartate shuttle involves the … The malate-aspartate shuttle involves the glutamate-aspartate carrier and the 2-oxoglutarate carrier exchanging malate2- for 2-oxoglutarate2-. Cytosolic and mitochondrial malate dehydrogenase and transaminase complete the shuttle for the transport of cytosolic NADH into the mitochondrial matrix. It is most important in heart, liver and kidney.chondrial matrix. It is most important in heart, liver and kidney.)
Mouse control: Mark + (The mark mode is active by default, can be … The mark mode is active by default, can be selected in the menu or by [Ctrl+M]. If '''Mouse control: Mark''' is enabled, specific sections of the experiment can be marked in each plot. Usually, marks are set on the plot for oxygen concentration for calibration, whereas marks on the plot for oxygen flux are set for exporting the median or average of flux to a table.»More details: [[Marks - DatLab]].[Marks - DatLab]].)
Wavelength range + (The minimum and the maximum wavelengths ov … The minimum and the maximum wavelengths over which an [[absorbance spectrum]] is measured are described in terms of the [[wavelength range]]. It is determined mainly by the specifications of the [[spectrophotometer]] and the type of [[light source]] used, and the characteristic [[absorbance spectrum]] of the sample being investigated.[[absorbance spectrum]] of the sample being investigated.)
Ergodynamics + (The mission of '''ergodynamics''' is the r … The mission of '''ergodynamics''' is the revelation of relations of general validity. "''Thermodynamics deals with relationships between properties of systems at equilibrium and with differences in properties between various equilibrium states. It has nothing to do with time. Even so, it is one of the most powerful tools of physical chemistry''" [1]. '''Ergodynamics''' is the theory of exergy changes (from the Greek word 'erg' which means [[work]]). Ergodynamics includes the fundamental aspects of thermodynamics ('[[heat]]') and the thermodynamics of irreversible processes (TIP; nonequilibrium thermodynamics), and thus links thermodynamics to kinetics. In its most general scope, ergodynamics is the science of [[energy]] transformations. Classical thermodynamics includes [[open system]]s, yet as a main focus it describes [[closed system]]s. This is reflected in a nomenclature that is not easily applicable to the more general case of open systems [2]. At present, IUPAC recommendations [3] fall short of providing adequate guidelines for describing energy transformations in open systems.ng energy transformations in open systems.)
Creatine kinase + (The mitochondrial '''creatine kinase''', also known as phosphocreatine kinase (CPK), facilitates energy transport with [[creatine]] and [[phosphocreatine]] as diffusible intermediates.)
Respiratory chain + (The mitochondrial '''respiratory chain''' … The mitochondrial '''respiratory chain''' (RC) consists of enzyme complexes arranged to form a metabolic system of convergent pathways for [[oxidative phosphorylation]]. In a general sense, the RC includes (1) the [[electron transfer pathway]] (ET-pathway), with transporters for the exchange of reduced substrates across the inner mitochondrial membrane, enzymes in the matrix space (particularly dehydrogenases of the tricarboxylic acid cycle), inner membrane-bound electron transfer complexes, and (2) the inner membrane-bound enzymes of the [[phosphorylation system]].[[phosphorylation system]].)
Mole + (The mole [mol] is the SI base unit for the … The mole [mol] is the SI base unit for the [[amount |amount of substance]] of a system that contains 6.02214076·1023 specified elementary entities (see [[Avogadro constant]]). The elementary entities must be specified and may be atoms, molecules, ions, electrons, other particles, or specified groups of such particles.ther particles, or specified groups of such particles.)
Pyruvate carrier + (The monocarboxylic acid [[pyruvate]]- is exchanged electroneutrally for OH- by the '''pyruvate carrier'''. H+/anion symport is equivalent to OH-/anion antiport.)
Drift + (The most common cause of '''drift''' is variation in the intensity of the [[light source]]. The effect of this can be minimised by carrying out a [[balance]] at frequent intervals.)
DatLab oxygen flux: performance and data analysis + (The quality of the results are strongly affected by the performance and data analysis. Therefore, we provide guidelines for performing and evaluating respirometric assays.)
Improvement score + (The relative improvement score, ''RIS'', p … The relative improvement score, ''RIS'', provides a measure of improvement of a trait from a value measured at baseline, ''B'', to a value measured after treatment, ''T'', expressing the total improvement, ''T-B'', in relation to the theoretical scope of improvement and the level of the trait observed at baseline. '''RIS'' incorporates the concept of diminishing returns and consideres maintaining a high value of a trait as an improvement relative to the potential loss.mprovement relative to the potential loss.)
Reproducibility crisis + (The reproducibility crisis is alarming.< … The reproducibility crisis is alarming.1 An experiment or study is ''reproducible'' or ''replicable'' when subsequent experiments confirm the results. This is [[research |re-search]]. However, we can define different types of reproducibility depending on the conditions that we use to replicate the previous work or in the information available. Our aim is to focus mostly on two different kinds2: '''1. Direct:''' is when we obtaining the same results using the same experimental conditions, materials, and methods as described in the original experiment. This would be the ideal reproducibility of an experiment. However, it requires a very accurate description of how the original experiment was performed. Some journals are trying to resolve the '''reproducibility crisis''' improving the rigor and the excellence on the reported methods and results (e.g. [https://www.cell.com/star-authors-guide STAR Methods in Cell Press]). '''2. Systematical:''' refers to obtaining the same results, but under different conditions; for example, using another cell line or mouse strain or humman study, or inhibiting a gene pharmacologically instead of genetically. This opens the door to subsequent studies to find the conditions under which an initial finding holds.udies to find the conditions under which an initial finding holds.)
Second + (The second, symbol s, is the SI unit of ti … The second, symbol s, is the SI unit of time. It is defined by taking the fixed numerical value of the caesium frequency ∆''ν''Cs, the unperturbed ground-state hyperfine transition frequency of the caesium 133 atom, to be 9 192 631 770 when expressed in the unit Hz, which is equal to s−1.ssed in the unit Hz, which is equal to s−1.)
Stoichiometric number + (The sign of the '''stoichiometric number'' … The sign of the '''stoichiometric number''' ''ν''X is determined by the nonspatial direction of the transformation (positive for products, negative for substrates), and the magnitude of ''ν''X is determined by the stoichiometric form. For instance, ''ν''A=-1 in the reaction 0 = -1 A + 2 B (-1 glucose converted to +2 lactate), but ''ν''A=-1/6 in the reaction 0 = -1/6 A - 1 B + 1 C (-1/6 glucose and -1 O2 converted to +1 H2CO3).1 B + 1 C (-1/6 glucose and -1 O2 converted to +1 H2CO3).)
Dithionite + (The sodium salt of '''Dithionite''' Na< … The sodium salt of '''Dithionite''' Na2S2O4 (Dit) is the 'zero oxygen solution powder' used for [[Oxygen calibration - DatLab |calibration of oxygen sensors]] at [[Zero calibration | zero oxygen concentration]], or for stepwise reduction of oxygen [[concentration]]s in [[MiPNet14.06 Instrumental O2 background |instrumental O2 background tests]]. It is not recommended to use dithionite in experiments with biological samples or several multisensor approaches, for these see [[Setting the oxygen concentration]].[[Setting the oxygen concentration]].)
Install Oroboros protocol package + (The standard '''Instrumental and SUIT DL-P … The standard '''Instrumental and SUIT DL-Protocols''' package is automatically implemented with the simple DatLab programme installation. We recommend a 'clean install': rename your previous DatLab programme subdirectory (''e.g.'' C:\DatLab_OLD).Updates and newly developed DL protocols can be simply downloaded by clicking on [Protocols]\Install Oroboros protocol package.tocols]\Install Oroboros protocol package.)
Flux analysis - DatLab + (The strategy of '''Flux analysis''' using … The strategy of '''Flux analysis''' using DatLab depends on the research question and the corresponding settings applied in DatLab when recording the data with the O2k. Usng [[MitoPedia: SUIT |SUIT protocols]], a sequence of respiratory steady-states is measured, marks are set, and numerical data are summarized in [[Mark statistics - DatLab|Mark statistics]] (F2). An AI approach is kept in mind when describing guidelines for evaluation of steady-states during data recording and analysis.states during data recording and analysis.)
% + (The symbol '''%''' indicates 'per cent' (per hundred). {''Quote''} The internationally recognized symbol % (per cent) may be used with the SI. When it is used, a space separates the number and the symbol %. {''end of Quote''}.)
≡ + (The symbol '''≡''' indicates (numerical) [[equivalence]], in contrast to = as the symbol for (physicochemical) [[equality]].)
Open chamber + (The term "open O2k-chamber" refers to a situation in which the liquid phase is allowed to equilibrate with a gas phase, but the stopper is partially inserted using the [[Stopper-Spacer]].)
Extroduction + (The term '''extroduction''' is ambiguous a … The term '''extroduction''' is ambiguous and needs introduction. An ''external'' extroduction aims at providing a specific exit that opens the door to the parent article. Once you popped up into the article box, there are various ''internal'' extroductions to push down by following hyperlinks to references, keywords, supplementary material, and to the external extroduction. Once you have pushed one level down, there may be hyperlinks to push down further ([[Hofstadter 1979 Harvester Press |Hofstadter 1979]]). One needs to keep track of the links in a nested network of open tabs, to pop up all the way back for returning to the initial reference level. returning to the initial reference level.)
Incident light + (The term '''incident light''' is used for a beam of light falling upon a surface.)
Isomorphic + (The term '''isomorphic''' refers to quanti … The term '''isomorphic''' refers to quantities which have [https://www.merriam-webster.com/dictionary/isomorphic ''identical or similar form, shape, or structure'']. In mathematics, an isomorphism defines a [https://www.merriam-webster.com/dictionary/isomorphism ''one-to-one correspondence between two mathematical sets'']. In [[ergodynamics]], isomorphic quantities are defined by equations of identical form. If isomorphic quantities are not expressed in identical units, then these quantities are expressed in different formats which can be converted to identical untis. Example: electric force [V=J/C] and chemical force [Jol=J/mol] are ismorphic [[force]]s; the electrical format [J/C] can be converted to the chemical format [J/mol] by the [[Faraday constant]]. Units not only give meaning to the numerical value of a quantity, but units provide also an abbreviated common language to communicate and compare isomorphic quantities. In irreversible thermodynamics, isomorphic forces are referred to as ''generalized'' forces.are referred to as ''generalized'' forces.)
System + (The term '''system''' has a variety of mea … The term '''system''' has a variety of meanings and dictionary definitions in different contexts, ''e.g.'', the [[International System of Units]] (SI), MKSA system, data management system, biological or mechanical system, redox system, [[Electron transfer system]], loosely or completely coupled system, instrumental system. In thermodynamics and [[ergodynamics]], the '''system''' is considered as an experimental system (experimental chamber), separated from the environment as an isolated, adiabatic, closed, or open system. {''Quote'' } The internal domain of any system is separated from the external domain (the surroundings) by a boundary. In theory, energy transformations outside the system can be ignored when describing the system. The surroundings are merely considered as a source or sink for quantities transferred across the system boundary. According to the transfer properties of the boundary, three types of thermodynamic systems are distinguished. (''1'') The boundaries of '''''isolated systems''''' are impermeable for all forms of [[energy]] and matter. Isolated systems do not interact with the surroundings. Strictly, therefore, internal changes of isolated systems cannot be observed from outside since any observation requires interaction. (''2'') The boundaries of '''''closed systems''''' are permeable for [[heat]] and [[work]], but impermeable for [[matter]]. A limiting case is electrons which cross the system boundary when work is exchanged in the form of electric energy [''added'': and light]. The volume of a closed system may be variable. (''3'') The boundaries of '''''open systems''''' allow for the transfer of heat, work and matter. Changes of isolated systems have exclusively internal origins, whereas changes of closed and open systems can be partitioned according to internal and external sources. Production and destruction of a quantity within the system are ''internal'' changes, whereas changes of heat, work and matter due to transfer across the system boundaries are labelled ''extenal''. (External) transfer is thus contrasted with (internal) production or destruction. {''end of Quote'': [[Gnaiger 1993 Pure Appl Chem]]}A system may be treated as a black box. In the analysis of [[Continuous system|continuous]] or [[Discontinuous system |discontinuous system]]s, however, information is implied on the internal structure of the system.d on the internal structure of the system.)
Hydrogen ion + (The terms '''hydrogen ion''' H+ … The terms '''hydrogen ion''' H+ and [[proton]], p or p+, are used synonymously in chemistry. A hydrogen ion is a positively charged molecule. In particle physics, however, a proton is a submolecular and subatomic particle with a positive electric charge. The H+ ion has no electrons and is a bare charge with only about 1/64 000 of the radius of a hydrogen atom. Free H+ is extremely reactive, with an extremely short lifetime in aqueous solutions. There H+ forms the hydronium ion H3O+, which in turn is further solvated by water molecules in clusters such as H5O2+ and H9O4+. The transfer of H+ in an acid–base reaction is referred to as ''proton transfer''. The acid is the H+ donor and the base is the H+ acceptor.lt;sup>+. The transfer of H+ in an acid–base reaction is referred to as ''proton transfer''. The acid is the H+ donor and the base is the H+ acceptor.)
Proton + (The terms '''proton''' p and [[hydrogen ion]] … The terms '''proton''' p and [[hydrogen ion]] H+ are used synonymously in chemistry. In particle physics, a proton is a subatomic particle with a positive electric charge. Protons and neutrons are collectively referred to as ''nucleons''. The proton is a bare charge with only about 1/64 000 of the radius of a hydrogen atom, and so the free proton is extremely reactive chemically. Therefore, the free proton has an extremely short lifetime in aqueous solutions where it forms the [[hydronium ion]], H3O+, which in turn is further solvated by water molecules in clusters such as H5O2+ and H9O4+.;sub>5O2+ and H9O4+.)
SI prefixes + (There are 20 '''SI prefixes''' defined to represent multiples and submiltiples of SI units.)
Sides + (There are many '''sides''' of the term 'si … There are many '''sides''' of the term 'side' in our language system. Inside and outside are the sides that are separated by the system boundaries of an experimental [[system]]. + and - are the two sides of numbers separated by 0. Pages in books have opposite sides or front sides versus backsides. Many fundamental terms have opposite sides of the meaning, thus spanning the entire message in the space between their apparently contrasting sides, and transforming the paradox as a perspective into the unified whole, the full, the complete. On the other side, such fundamental terms are fully understood only after ''realization'' of the opposite sides of their meaning — treasures discovered in the etymological origins of the word. It makes sense to open all our senses to comprehend the bright side and the dark side of things. Whereas the student sais "I see a black sheep", Zen decides "You see, that one side of the sheep is black". This is the message to consider both sides before choosing sides, besides overcoming a one-sided point of view. Don't rock side-to-side, but get immersed deeply inside things to see the upsides and downsides of every thing or anything, and more so of nothing. Inside is the insight, for insiders and outsiders of the feedback loop of an [[Ouroboros]].Ouroboros]].)
DatLab and SUIT protocols + (This is a brief summary of steps to be tak … This is a brief summary of steps to be taken for performing a high-resolution respirometry experiment with '''[[SUIT protocols]]''' using the OROBOROS [[Oroboros O2k]] and '''[[DatLab]]''' software. (1) Search for a specific [[SUIT protocol name]] (go to [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT]]). The list of MitoPedia SUIT protocols can be sorted by [[categories of SUIT protocols]] (sorting by SUIT protocol name), which is listed as the 'abbreviation' of the SUIT protocol name. (2) Copy the template for [[Mark names]] into your DatLab subdirectory: DatLab\APPDATA\MTEMPLAT. (3) Copy the [[DatLab-Analysis templates |DatLab-Analysis template]] for this SUIT protocol. (4) Follow the link to the corresponding publication or MiPNet communication, where the pdf file describing the SUIT protocol is available. (5) A DatLab demo file may be available providing an experimental example. After each sequential titration, a mark is set on the plot for flux or flow. After having set all marks, pull down the 'Mark names' menu, select the corresponding SUIT protocol for mark names, and rename all marks. The Mark names template also provides standard values of the titration volume preceding each mark. (6) Go to 'Mark statistics' [F2], copy to clipboard, and paste into the sample tab in the DatLab-Analysis template.: Example::* SUIT protocol name: [[SUIT-011]]:* Mark names in DatLab: 1GM;2D;2c;3S;4U;5Rot-:* DatLab-Analysis template: SUIT_NS(GM)01.xlsx:* MiPNet communciation: [[MiPNet12.23 FibreRespiration]]:* DatLab demo file: MiPNet12.23 FibreRespiration.DLDemo file: MiPNet12.23 FibreRespiration.DLD)
Beer-Lambert law + (This law states that the [[transmittance]] … This law states that the [[transmittance]] (''T'') of light though a sample is given by:''T'' = e-''εbc'', where ''ε'' is the molar [[extinction coefficient]], ''b'' is the pathlength of the light through the cuvette (in mm) and ''c'' is the concentration of the pigment in the sample (in mM). Transforming this equation, it can be seen that the [[absorbance]] of light (''A'') is simply given by ''A'' = ''εbc''.absorbance]] of light (''A'') is simply given by ''A'' = ''εbc''.)
Least squares method + (This method makes use of all of the data p … This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spectrum of known concentration using a '''least squares method''' to match the measured spectrum with the reference spectrum. The technique results in improved accuracy compared with the use of only a few characteristic wavelengths. of only a few characteristic wavelengths.)
Paywall journalism + (Though often defined from the individual r … Though often defined from the individual reader's perspective, a paywall can also apply to an institution (such as a library) or the author. '''Paywall journalism''' is the opposite of [[Open Access]]. [[Open Science]] does not accept paywalls with the argument, that the public pays for governmentally funded research, hence research funded by public grants should be published with open access for the public without paywalls. Paywalls are most frequently defined from the perspective of the individual reader, who has to pay for an article or pay a journal subscription as a requisite for obtaining full access to the information that is otherwise hidden behind the paywall ('''reader-paywall journal'''). From the perspective of the authors, however, an '''author-paywall journal''' is defined as any journal which requests publication charges or page charges from the authors for publishing the manuscript Open Access or publishing it at all. Similarly, an '''institutional-paywall journal''' charges an institution – typically university libraries – for granting open access to the members of this institution. As long as paywall journalism prevails in science, at least '''paywall transparence''' should be required, to declare for each publication not only the reader-paywall costs but provide the full information on the author-paywall and institutional-paywall expenses.aywall and institutional-paywall expenses.)
O2k signals and output + (Three electronic '''channel types''' are a … Three electronic '''channel types''' are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]. All channels are available twofold (dual-data), for O2k-Chambers A (left) and B (right), based on numerical signals sent at a fixed data sampling time interval (default: 2 s; range 0.2 s to >10 s).rval (default: 2 s; range 0.2 s to >10 s).)
Stirrer A on/off + (Toggles between stirrer on/off in the left O2k-chamber, returning to the pre set stirrer speed.)
Stirrer B on/off + (Toggles between stirrer on/off in the right O2k-chamber, returning to the pre set stirrer speed.)
Triethyltin bromide + (Triethyltin bromide (TET) is a lipophilic [1] inhibitor of the mitochondrial [[ATP synthase]] [2] which is used to induce [[LEAK state]] in [[living cells]] of ''Saccharomyces cerevisiae''.)
POS calibration - static + (Two-point calibration of the polarographic oxygen sensor, comprising [[Air calibration]] and [[Zero calibration]]. See also [[POS calibration - dynamic]].)
Unspecific binding of TPP+ + (Unspecific binding of the probe molecule T … Unspecific binding of the probe molecule TPP+ in the matrix phase of mitochondria is taken into account as a correction for measurement of the [[mitochondrial membrane potential]]. External unspecific binding is the binding outside of the inner mt-membrane or on the outer side of the inner mt-membrane, in contrast to internal unspecific binding.-membrane, in contrast to internal unspecific binding.)
SUITbrowser + (Use the '''SUITbrowser''' to find the subs … Use the '''SUITbrowser''' to find the substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocol most suitable for addressing your research questions. <big><big>Open the SUITbrowser: http://suitbrowser.oroboros.at/</big></big>[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]w.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)])
Getting started - DatLab + (Users have to enter their user details the first time they use DatLab 8 on a specific computer. As well, entering some basic settings is required when connecting DatLab 8 with an O2k for the first time.)
Valinomycin + (Valinomycin catalyzes electrogenic K+ transport down the electrochemical transmembrane gradient (150 ng.mg-1 protein).)
Smoothing + (Various methods of '''smoothing''' can be … Various methods of '''smoothing''' can be applied to improve the [[signal-to-noise ratio]]. For instance, data points recorded over time [s] or over a range of wavelengths [nm] can be smoothed by averaging ''n'' data points per interval. Then the average of the ''n'' points per smoothing interval can be taken for each successively recorded data point across the time range or range of the spectrum to give a ''n''-point moving average smoothing. This method decreases the [[noise]] of the signal, but clearly reduces the time or wavelength [[resolution]]. More advanced methods of smoothing are applied to retain a higher [[time resolution]] or wavelength resolution.[[time resolution]] or wavelength resolution.)
Hydrogenion flux + (Volume-specific '''hydrogenion flux''' or … Volume-specific '''hydrogenion flux''' or H+ flux is measured in a closed system as the time derivative of H+ concentration, expressed in units [pmol·s-1·mL-1]. H+ flux can be measured in an open system at steady state, when any acidification of the medium is compensated by external supply of an equivalent amount of base. The extracellular acidification rate (ECAR) is the change of pH in the incubation medium over time, which is zero at steady state. Volume-specific H+ flux is comparable to volume-specific [[oxygen flux]] [pmol·s-1·mL-1], which is the (negative) time derivative of oxygen concentration measured in a closed system, corrected for instrumental and chemical background.[[pH]] is the negative logarithm of hydrogen ion activity. Therefore, ECAR is of interest in relation to acidification issues in the incubation buffer or culture medium. The physiologically relevant metabolic H+ flux, however, must not be confused with ECAR.e incubation buffer or culture medium. The physiologically relevant metabolic H+ flux, however, must not be confused with ECAR.)
Different O2 fluxes in left and right chamber + (What are potential causes for '''different O2 fluxes in the left and right chamber'''?)
Transmittance + (When light enter a sample, '''transmittance''' (''T'') is the fraction of the intensity (''I'') of the light emerging from the sample compared with the incident light intensity (''I''''0''): ''T'' = ''I''/''I''''0''.)
Absorption + (When light enters a sample and emerges wit … When light enters a sample and emerges with an intensity (''I''), '''absorption''' (''Abs'') is the fraction of the light absorbed by the sample compared with the [[incident light]] intensity (''I''''0''): ''Abs'' = 1-''I''/''I''''0''. Absorption can also be expressed as ''Abs'' = 1-''T'', where ''T'' is the [[transmittance]].[[transmittance]].)
Absorbance spectrum + (When light enters a sample, the amount of … When light enters a sample, the amount of light that it absorbs is dependent upon the wavelength of the incident light. The '''absorbance spectrum''' is the curve derived by plotting the measured [[absorbance]] against the wavelength of the light emerging from the sample over a given [[wavelength range]]. An [[absorbance spectrum]] may be characterised by peaks and troughs (absorbance maxima and minima) that can be used to identify, and sometimes quantify, different absorbing substances present in a sample. absorbing substances present in a sample.)
O2k-MultiSensor + (When one (or more) analytical parameters a … When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux, this is an '''O2k-MultiSensor''' application of the [[Oroboros O2k-technology]]. The [[NextGen-O2k]] supports all O2k-MultiSensor Modules, while the O2k does not provide for the Q- and NADH-Redox-Modules. For some O2k-MultiSensor applications it is necessary to introduce one or more additional sensors into the chamber through a MultiSensor stopper. Optical applications require the standard black stoppers.tions require the standard black stoppers.)
TIP2k syringe blocked + (When the '''TIP2k syringe is blocked''', it must not be used with the TIP2k, and specific cleaning instructions should be followed.)
Living Communications + (With '''Living Communications''', [https:/ … With '''Living Communications''', [https://www.bioenergetics-communications.org/index.php/bec Bioenergetics Communications] (BEC) takes the next step from pre-print to re-print. The concept of ''Living Communications'' pursues a novel culture of scientific communication, addressing the conflict between long-term elaboration and validation of results versus sharing without delay improved methods and preliminary findings. Following the preprint concept, updates may be posted on the BEC website of the resource publication. Updated versions of Living Communications are submitted for Open Peer Review with full traceability. In contrast to static papers, evolution of ''Living Communications'' is more resourceful and efficient than a ‘new’ publication. ''Living Communications'' provide a pathway along the scientific culture of lively debate towards tested and trusted milestones of research, from pre-print to re-print, from initial steps to next steps.e-print, from initial steps to next steps.)
Internal flow + (Within the system boundaries, irreversible … Within the system boundaries, irreversible '''internal flows''', ''I''i,—including chemical reactions and the dissipation of internal gradients of heat and matter—contribute to internal entropy production, di''S''/d''t''. In contrast, [[external flow]]s, ''I''e, of heat, work, and matter proceed reversibly across the system boundaries (of zero thickness). Flows are expressed in various [[format]]s per unit of time, with corresponding [[motive unit]]s [MU], such as chemical [mol], electrical [C], mass [kg]. Flow is an [[extensive quantity]], in contrast to [[flux]] as a [[specific quantity]].ecific quantity]].)
Liver mitochondria purification + ([[Armstrong 2010 J Comp Physiol B]]: This paper describes a method for purification of rodent liver mitochdondria using relatively low-speed centrifugation through discontinuous Percoll gradients.)
File:MitoFitPreprints and BEC manuscript template.docx + ([[Bioenergetics Communications]] and [[MitoFit Preprints]] manuscript template.)
ET-pathway competent state + ([[Electron transfer pathway]] competent state, ''see'' '''[[Electron-transfer-pathway state]]'''.)
Duroquinol + ([[Electron-transfer-pathway state |ET-path … [[Electron-transfer-pathway state |ET-pathway level 2]] is supported by '''duroquinol''' DQ feeding electrons into Complex III (CIII) with further electron transfer to CIV and oxygen. Upstream pathways are inhibited by rotenone and malonic acid in the absence of other substrates linked to ET-pathways with entry into the Q-junction.T-pathways with entry into the Q-junction.)
Fluorometric dyes + ([[Extrinsic fluorophores]]; fluorescent markers.)
SUIT-014 + ([[File: 1GM;2D;3P;4S;5U;6Rot-.png|400px]])
O2k-sV-Module + ([[File:11200-01.jpg|180px|right]] The ''' … [[File:11200-01.jpg|180px|right]] The '''O2k-sV-Module''' is the O2k small-volume module, comprised of two Duran® glass chambers of 12 mm inner diameter specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all the components necessary for a smaller operation volume ''V'' of 0.5 mL. The current DatLab version is included in the delivery of this revolutionary module.the delivery of this revolutionary module.)
SUIT-033 + ([[File:1D.1;2PGM;3D2.5-.png|450px]])
SUIT-038 O2 mt D091 + ([[File:1D;2M.1;2H2O;2c;3M.2;3M.5;3M1;3M2;4P;5G;6S10;6S50;7Gp;8U;9Rot;10Ama.png|400px]])
SUIT-041 O2 mt D096 + ([[File:1D;2M.1;3AC;3c;4M2;5P;6S;7Rot;8Ama.png|400px]])
SUIT-037 O2 mt D090 + ([[File:1D;2M.1;3Oct;3c;4M.2;4M.5;4M1;4M2;5P;6G;7S10;7S50;8Gp;9U;10Rot;11Ama.png|400px]])
SUIT-002 O2 mt D005 + ([[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px]])
SUIT-025 + ([[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Rot-.png|600px]])
SUIT-025 O2 mt D057 + ([[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Rot;9Ama.png|600px]])
SUIT-002 + ([[File:1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U;10Rot-.png|400px]])
SUIT-036 O2 mt D089 + ([[File:1D;2M.1;3Pal;3c;4M.2;4M.5;4M1;4M2;5P;6G;7S10;7S50;8Gp;9U;10Rot;11Ama.png|400px]])
SUIT-040 O2 mt D094 + ([[File:1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama.png|400px]])
SUIT-040 O2 pfi D095 + ([[File:1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama.png|400px]])
SUIT-039 O2 mt D092 + ([[File:1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8U;9Rot;10Ama.png|400px]])
SUIT-039 O2 pfi D093 + ([[File:1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8U;9Rot;10Ama.png|400px]])
SUIT-007 + ([[File:1G;2D;3M;4U-.png|300px]])
SUIT-014 O2 pfi D042 + ([[File:1GM;2D;2c;3P;4S;5U;6Rot;7Ama.png|400px]])
SUIT-021 O2 mt D035 + ([[File:1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama.png|300px]])
SUIT-011 O2 pfi D024 + ([[File:1GM;2D;2c;3S;4U;5Rot;6Ama.png|400px]])
SUIT-021 + ([[File:1GM;2D;3S;4Rot;5Omy;6U-.png|400px]])
SUIT-021 Fluo mt D036 + ([[File:1GM;2D;3S;4Rot;5Omy;6U;7Ama.png|300px]])
SUIT-011 + ([[File:1GM;2D;3S;4U;5Rot-.png|400px|SUIT-011]])
SUIT-018 + ([[File:1GMS;2D-.png|300px|SUIT-018]])
SUIT-018 O2 mt D054 + ([[File:1GMS;2D;2c;3Ama.png|290px]])
SUIT-018 AmR mt D031 + ([[File:1GMS;2D;3Ama.png|290px]])
SUIT-018 AmR mt D041 + ([[File:1GMS;2D;3Ama.png|290px]])
SUIT-018 AmR mt D040 + ([[File:1GMS;2D;3Ama.png|290px|SUIT-018]])
SUIT-027 + ([[File:1M;2D;3M;4P;5G-.png|400px]])
SUIT-017 + ([[File:1OctM;2D;2c;3G;4S;5U;6Rot-.png |355px]])
SUIT-017 O2 pfi D049 + ([[File:1OctM;2D;2c;3G;4S;5U;6Rot;7Ama.png|350px]])
SUIT-005 O2 pfi D011 + ([[File:1OctM;2D;2c;3P;4S;5U;6Rot;7Ama;8AsTm;9Azd.png|450px]])
SUIT-017 O2 mt D046 + ([[File:1OctM;2D;3G;3c;4S;5U;6Rot;7Ama.png |350px]])
SUIT-015 + ([[File:1OctM;2D;3G;4P;5S;6U;7Rot-.png|451px]])
SUIT-015 O2 pti D043 + ([[File:1OctM;2D;3G;4P;5S;6U;7Rot;8Ama.png|450px]])
SUIT-016 + ([[File:1OctM;2D;3G;4S;5Rot;6Omy;7U-.png|420px]])
SUIT-016 O2 pfi D044 + ([[File:1OctM;2D;3G;4S;5Rot;6Omy;7U;7c-8Ama.jpg|400px]])
SUIT-005 + ([[File:1OctM;2D;3P;4S;5U;6Rot-.png|300px]])
1OctM;2D;3PG;4S;5U;6Rot- + ([[File:1OctM;2D;3PG;4S;5U;6Rot-.png|300px]])
1PGM;2D;3S;4Rot;5U- + ([[File:1PGM;2D;3S;4Rot;5U-.png|300px]])
SUIT-028 + ([[File:1PGM;2D;3S;4U;5Rot-.png|400px|SUIT-028]])

1PGM;2D;3U;4S;5Rot- + ([[File:1PGM;2D;3U;4S;5Rot-.png|300px]])

SUIT-020 O2 mt D032 + ([[File:1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8Ama.png|500px]])
SUIT-008 O2 pfi D014 + ([[File:1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.png|400px]])
SUIT-008 O2 mt D026 + ([[File:1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.png|600px]])
SUIT-012 O2 mt D027 + ([[File:1PM;2D;2c;3G;4U;5Ama.png|300px]])
SUIT-006 O2 mt D047 + ([[File:1PM;2D;2c;3Omy;4U;5Ama.png|300px]])
SUIT-031 O2 mt D075 + ([[File:1PM;2D;2c;3S;4Rot;5U;6Ama.png|400px]])
SUIT-001 + ([[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp-.png|400px|SUIT-001]])
SUIT-004 O2 pfi D010 + ([[File:1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8Azd.png|450px]])
SUIT-006 MgG mt D055 + ([[File:1PM;2D;3Cat;4U;5Ama.png|300px]])
SUIT-020 + ([[File:1PM;2D;3G;4S;5Rot;6Omy;7U-.png|400px]])
SUIT-020 Fluo mt D033 + ([[File:1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama.png|450px]])
SUIT-008 O2 pce D25 + ([[File:1PM;2D;3G;4S;5U;6Rot-.png|300px]])
SUIT-008 + ([[File:1PM;2D;3G;4S;5U;6Rot.png|400px]])
SUIT-012 + ([[File:1PM;2D;3G;4U-.png|300px]])
1PM;2D;3G;4U;5S;6Rot- + ([[File:1PM;2D;3G;4U;5S;6Rot-.png|300px]])
SUIT-006 Fluo mt D034 + ([[File:1PM;2D;3Omy;4U;5Ama.png|300px]])
SUIT-006 AmR mt D048 + ([[File:1PM;2D;3Omy;4U;5Ama.png|400px]])
SUIT-031 + ([[File:1PM;2D;3S;4Rot;5U;-.png|400px]])
SUIT-004 + ([[File:1PM;2D;3U;4S;5Rot.png|450px]])
SUIT-029 O2 mt D066 + ([[File:1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot;8Ama.png|350px]])
SUIT-019 O2 pfi D045 + ([[File:1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama.png|400px]])
SUIT-019 + ([[File:1PalM;2D;3Oct;4P;5G;6U;7S;8Rot-.png|450px]])
SUIT-009 O2 mt D015 + ([[File:1S;2D;2c;3P;4Rot;5Ama.png|400px|SUIT9]])
SUIT-009 + ([[File:1S;2D;3P;4Rot-.png|400px|SUIT9]])
SUIT-009 AmR mt D021 + ([[File:1S;2D;3P;4Rot;5Ama.png|400px|SUIT-009]])
SUIT-026 O2 mt D063 + ([[File:1S;2Rot;3D;3c;4Ama.png|400px]])
SUIT-026 + ([[File:1S;2Rot;3D;4Ama.png|350px]])
SUIT-006 O2 mt D022 + ([[File:1SRot;2D;2c;3(Omy);4U;5Ama.png|400px]])
SUIT-006 + ([[File:1X;2D;2c;3Omy;4U-.png|450px]])
SUIT-003 O2 ce D009 + ([[File:1ce;2ceOmy;3ceU-.jpg|450px]])
SUIT-003 Ce1;ce2U- + ([[File:1ce;2ceU-.jpg|150px]])
SUIT-003 Ce1;ce3U- + ([[File:1ce;3ceU-.jpg|150px]])
SUIT-032 NADH mt D078 + ([[File:1mt;1PGM;2D;3Anox;4Myx;5Reox.png|300px]])
Oxoglutarate + ([[File:2-Oxoglutaric_acid.jpg|left|100px|2 … [[File:2-Oxoglutaric_acid.jpg|left|100px|2-Oxoglutaric acid]]'''2-Oxoglutaric acid''' or alpha-ketoglutaric acid, C5H6O5, occurs under physiological conditions as the anion '''2-Oxoglutarate2-, Og'''. 2-Oxoglutarate (alpha-ketoglutarate) is formed from isocitrate as a product of [[isocitrate dehydrogenase]] (IDH) in the [[TCA cycle]], and is a substrate of [[oxoglutarate dehydrogenase]] (OgDH). The 2-oxoglutarate carrier exchanges malate2- for 2-oxoglutarate2- as part of the [[malate-aspartate shuttle]]. In the cytosol, oxoglutarate+aspartate are transaminated to form oxaloacetate+glutamate. Cytosolic malate dehydrogenase converts oxaloacetate+NADH to malate.transaminated to form oxaloacetate+glutamate. Cytosolic malate dehydrogenase converts oxaloacetate+NADH to malate.)
Mitochondria-Targeted Drug Development + ([[File:21640 - Mitochondria Targeted Thera … [[File:21640 - Mitochondria Targeted Therapeutics logo NEW.jpg|200px|left|Mitochondria-Targeted Drug Development]]The '''Mitochondria-Targeted Drug Development Summit''' was first established in 2021, as an online conference. Due to its success and unmatched focus, the 2nd edition returns to Boston this March 2022. This is the only industry-led meeting that unites key stakeholders under a mutual and ambitious objective of '''accelerating the discovery and development of novel drugs that target mitochondrial functions''' for chronic, primary mitochondrial diseases, muscular dystrophy, metabolic disorders, and neurodegenerative diseases.Join our speakers from '''GenSight Biologics, Abliva, Reneo Pharma, Mito BioPharma, Mitokinin''' and more with exciting networking opportunities, panel discussions and dedicated roundtables.tunities, panel discussions and dedicated roundtables.)
Screwdriver allen wrench + ([[File:24330-02.jpg|right|180px]]'''Screwdriver allen wrench''', a standard component of the [[O2k-FluoRespirometer]] and [[O2k-sV-Module]].)
MultiSensor-Connector + ([[File:30420-24 MultiSensorConnector.JPG|right|180px]] '''MultiSensor-Connector''': for separate reference electrode and [[ISE]]; only for O2k-Series B and Series C with MultiSensor electronic upgrading before 2011.)
MultiSensor-Preamplifier 1/100 + ([[File:30430--24 NO-Attachment.JPG|right|180px]] '''MultiSensor-Preamplifier 1/100''': Required only for O2k-Series A-C, for application of NO (or other amperometric) sensors (single chamber mode of application).)
TIP2k-Needle Safety Support with cable guide + ([[File:31330-01 3.jpg|right|280px]] '''TIP2k-Needle Safety Support with cable guide''': for safe storage of TIP2k-needles and MultiSensor Modules cables when not required during the experiment.)
Storage box sV + ([[File:32001-01.jpg|right|180px]]'''Storage box sV''' empty, for storage of [[O2k-sV-Module]] components.)
O2k-Chamber Holder sV + ([[File:32100-01.jpg|right|180px]]'''O2k-Chamber Holder sV''' (black POM) for PVDF or PEEK stoppers (0.5-mL [[O2k-chamber]]), with [[O-ring\Viton\16x2 mm]] and [[V-ring\30-35-4.5 mm]].)
POS-Holder sV + ([[File:32300-01.jpg|right|180px]] '''POS-H … [[File:32300-01.jpg|right|180px]]'''POS-Holder sV''', made from black POM, to be screwed into the copper block of the [[O2k-Main Unit]], guiding the [[OroboPOS|POS]] to the [[O2k-Chamber sV]], and keeping the SmartPOS/OroboPOS-Connector in a fixed position for sealing the O2k-Chamber sV with the [[POS-Seal Tip]]. In addition, the POS-Holder sV fixes the O2k-Chamber sV in an accurate rotational position by pressing against the angular cut of the glass chamber.inst the angular cut of the glass chamber.)
O2k-Chamber sV + ([[File:33100-01.jpg|right|180px]]'''O2k-Chamber sV''': 12 mm inner diameter, Duran® glass polished, with standard operation volume ''V'' of 0.5 mL.)
Stirrer-Bar sV\white PVDF\11.5x6.2 mm + ([[File:33210-01.jpg|right|180px]]'''Stirre … [[File:33210-01.jpg|right|180px]]'''Stirrer-Bar sV\white [[PVDF]]\11.5x6.2 mm''', operated in the 0.5-mL [[O2k-Chamber sV]] at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor ([[OroboPOS]]) placed in a position of maximum current of the medium.position of maximum current of the medium.)
Stopper sV\black PEEK\conical Shaft\central Port + ([[File:34000-01.jpg|right|180px]]'''Stoppe … [[File:34000-01.jpg|right|180px]]'''Stopper sV\black PEEK\conical Shaft\central Port''': with conical shaft (with PTFE, graphite, carbon fiber) and one central capillary (1.0 mm diameter; 48.9 mm length), [[Volume-Calibration Ring sV]] (A or B) for volume adjustment 0.5 mL; 2 mounted O-rings ([[O-ring sV\Viton\9.5x1 mm]]).[[O-ring sV\Viton\9.5x1 mm]]).)
O-ring sV\Viton\9.5x1 mm + ([[File:34310-02.jpg|right|180px]]'''O-ring sV\[[Viton]]\9.5x1 mm''', for [[Stopper sV\black PEEK\conical Shaft\central Port | PEEK Stopper sV]], 2 are mounted on each PEEK Stopper sV, box of 8 as spares.)
Asia Society for Mitochondrial Research and Medicine + ([[File:ASMRM LOGO.JPG|200px|left]]The '''Asia Society for Mitochondrial Research and Medicine''' (ASMRM) was founded in 2003 to share the latest knowledge on mitochondrial research.)
Acetyl-CoA + ([[File:Acetyl coenzyme A 700.png|left|200p … [[File:Acetyl coenzyme A 700.png|left|200px|acetyl-CoA]]'''Acetyl-CoA''', C23H38N7O17P3S, is a central piece in metabolism involved in several biological processes, but its main role is to deliver the acetyl group into the [[TCA cycle]] for its oxidation. It can be synthesized in different pathways: (i) in glycolysis from [[pyruvate]], by pyruvate dehydrogenase, which also forms NADH; (ii) from fatty acids β-oxidation, which releases one acetyl-CoA each round; (iii) in the catabolism of some amino acids such as leucine, lysine, phenylalanine, tyrosine and tryptophan. In the mitochondrial matrix, acetyl-CoA is condensed with [[oxaloacetate]] to form [[citrate]] through the action of [[citrate synthase]] in the [[tricarboxylic acid cycle]]. Acetyl-CoA cannot cross the mitochondrial inner membrane but citrate can be transported out of the mitochondria. In the cytosol, citrate can be converted to acetyl-CoA and be used in the synthesis of fatty acid, cholesterol, ketone bodies, acetylcholine, and other processes. and be used in the synthesis of fatty acid, cholesterol, ketone bodies, acetylcholine, and other processes.)
Aconitase + ([[File:Aconitase.jpg|right|500px|aconitase … [[File:Aconitase.jpg|right|500px|aconitase]]'''Aconitase''' is a [[TCA cycle]] enzyme that catalyzes the reversible isomerization of [[citrate]] to [[isocitrate]]. Also, an isoform is also present in the cytosol acting as a trans-regulatory factor that controls iron homeostasis at a post-transcriptional level. tasis at a post-transcriptional level. )
Cardiovascular Exercise Research Group + ([[File:CERG.gif|200px|left|CERG]] The '''C … [[File:CERG.gif|200px|left|CERG]]The '''Cardiovascular Exercise Research Group''' (CERG) was established in January 2008 and their research focuses on identifying the key cellular and molecular mechanisms underlying the beneficial effects of physical exercise on the heart, arteries and skeletal muscle in the context of disease prevention and management through experimental, clinical and epidemiological studies. Since 2003 this research group organizes the biennial seminar [http://www.ntnu.edu/cerg/seminar-2013 "Exercise in Medicine"] in Trondheim, Norway.ercise in Medicine"] in Trondheim, Norway.)
Complex II ambiguities + ([[File:CII-ambiguities Graphical abstract. … [[File:CII-ambiguities Graphical abstract.png|300px|left|link=Gnaiger 2023 MitoFit CII]]The current narrative that the reduced coenzymes NADH and FADH2 feed electrons from the tricarboxylic acid (TCA) cycle into the mitochondrial electron transfer system can create ambiguities around respiratory Complex CII. Succinate dehydrogenase or CII reduces FAD to FADH2 in the canonical forward TCA cycle. However, some graphical representations of the membrane-bound electron transfer system (ETS) depict CII as the site of oxidation of FADH2. This leads to the false believe that FADH2 generated by electron transferring flavoprotein (CETF) in fatty acid oxidation and mitochondrial glycerophosphate dehydrogenase (CGpDH) feeds electrons into the ETS through CII. In reality, NADH and succinate produced in the TCA cycle are the substrates of Complexes CI and CII, respectively, and the reduced flavin groups FMNH2 and FADH2 are downstream products of CI and CII, respectively, carrying electrons from CI and CII into the Q-junction. Similarly, CETF and CGpDH feed electrons into the Q-junction but not through CII. The ambiguities surrounding Complex II in the literature call for quality control, to secure scientific standards in current communications on bioenergetics and support adequate clinical applications.nd support adequate clinical applications.)
Complex II + ([[File:CII.png |right|200px|link=Gnaiger 2 … [[File:CII.png |right|200px|link=Gnaiger 2023 MitoFit CII]]'''Complex II''' or '''succinate:quinone oxidoreductase (SQR)''' is the only membrane-bound enzyme in the [[TCA cycle]] and is part of the [[electron transfer pathway]]. The reversible oxidoreduction of succinate and fumarate is catalyzed in a soluble domain and coupled to the reversible oxidoreduction of quinol and quinone in the mitochondrial inner membrane. CII consists in most species of four subunits. The flavoprotein [[succinate dehydrogenase]] is the largest polypeptide of CII, located on the matrix face of the mt-inner membrane. Succinate:quinone oxidoreductases (SQRs, SDHABCD) favour oxidation of succinate and reduction of quinone in the canonical forward direction of the TCA cycle and electron transfer into the [[Q-junction]]. In contrast, quinol:fumarate reductases (QFRs, fumarate reductases, FRDABCD) tend to operate in the reverse direction reducing fumarate and oxidizing quinol.on reducing fumarate and oxidizing quinol.)
SUIT-007 O2 ce-pce D030 + ([[File:Ce1;1Dig;1G;2D;2c;3M;4U;5Ama.png|400px]])
SUIT-027 O2 ce-pce D065 + ([[File:Ce1;1Dig;1M;2D;3M;4P;5G;6Ama.png|500px]])
SUIT-006 O2 ce-pce D029 + ([[File:Ce1;1Dig;1PM;2D;2c;3Omy;4U;5Ama.png|600px]])
SUIT-031 O2 ce-pce D079 + ([[File:Ce1;1Dig;1PM;2D;2c;3S;4Rot;5U;6Ama.png|400px]])
SUIT-024 + ([[File:Ce1;1Dig;1PM;2T;2D;3Omy-.png|410px]])
SUIT-024 O2 ce-pce D056 + ([[File:Ce1;1Dig;1PM;2T;2D;3Omy;4Ama.png|410px]])
SUIT-031 Q ce-pce D074 + ([[File:Ce1;1Dig;1Q2;1PM;2D;3S;4Rot;5U;6Anox;7Ama.png|400px]])
SUIT-009 O2 ce-pce D016 + ([[File:Ce1;1Dig;1S;2D;2c;3P;4Rot;5Ama.png|520px|SUIT-009]])
SUIT-009 AmR ce-pce D019 + ([[File:Ce1;1Dig;1S;2D;3P;4Rot;5Ama.png|520px|SUIT9]])
SUIT-026 AmR ce-pce D087 + ([[File:Ce1;1Dig;1S;2Rot;3D;4Ama.png|600px]])
SUIT-018 AmR ce-pce D068 + ([[File:Ce1;1Dig;2GMS;3D;4Ama.png|290px]])
SUIT-003 O2 ce D037 + ([[File:Ce1;ce1Glc;ce2(Omy);ce3U;ce4Ama.png|350px]])
SUIT-003 O2 ce-pce D018 + ([[File:Ce1;ce1P;ce2Omy;ce3U;ce4Glc;ce5Rot;ce6S;1Dig;1U;1c;2Ama;3AsTm;4Azd.png|600px]])
SUIT-003 O2 ce D012 + ([[File:Ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5Ama.png|400px]])
SUIT-003 Ce1;ce1SD;ce3U;ce4Rot;ce5Ama + ([[File:Ce1;ce1SD;ce3U;ce4Rot;ce5Ama.png|200px]])
SUIT-003 + ([[File:Ce1;ce2(Omy);ce3U-.png|250px]] [[File:Ce5S;1Dig;1c-.png|250px]])
SUIT-003 O2 ce D038 + ([[File:Ce1;ce2(Omy);ce3U;ce3Glc;ce3'U;ce4Ama.png|350px]])
SUIT-003 Ce1;ce2U;ce3Rot;ce4S;ce5Ama + ([[File:Ce1;ce2U;ce3Rot;ce4S;ce5Ama.png|200px]])
SUIT-003 Ce1;ce3U;ce4Rot;ce5S;ce6Ama + ([[File:Ce1;ce3U;ce4Rot;ce5S;ce6Ama.png|200px]])
Cell Symposia + ([[File:CellSymposiaLogo.jpg|90px]] Organized by the editors of Cell Press's leading journals, '''Cell Symposia''' bring together exceptional speakers and scientists to discuss topics at the forefront of scientific research.)
Chemical background + ([[File:Chb.png|100px|https://wiki.oroboros … [[File:Chb.png|100px|https://wiki.oroboros.at/index.php/File:Chb.png]] '''Chemical background''' ''Chb'' is due to autooxidation of the reagents. During CIV assays, ascorbate and TMPD are added to maintain cytochrome ''c'' in a reduced state. External cytochrome ''c'' may be included in the CIV assay. The autooxidation of these compounds is linearly oxygen-dependent down to approximately 50 µM oxygen and responsible for the chemical background oxygen flux after the inhibition of CIV. Oxygen flux due to the chemical reaction of autooxidation must be corrected for the [[Oxygen flux - instrumental background|instrumental O2 background]]. The correction for chemical background is necessary to determine CIV activity, in which case the instrumental O2 background and chemical background may be combined in an overall correction term.be combined in an overall correction term.)
Citrate + ([[File:Citrate 300 (1).png|left|100px|citr … [[File:Citrate 300 (1).png|left|100px|citrate]]'''citrate''', C6H5O7-3, is a tricarboxylic acid trianion, intermediate of the TCA cycle, obtained by deprotonation of the three carboxy groups of citric acid. Citrate is formed from [[oxaloacetate]] and acetyl-CoA through the catalytic activity of the [[citrate synthase]]. In the TCA cycle, citrate forms isocitrate by the activity of the [[aconitase]]. Citrate can be transported out of the mitochondria by the tricarboxylate transport, situated in the inner mitochondrial membrane. The transport occurs as an antiport of malate from the cytosol and it is a key process for fatty acid and oxaloacetate synthesis in the cytosol. ol and it is a key process for fatty acid and oxaloacetate synthesis in the cytosol. )
Coenzyme Q2 + ([[File:Coenzyme Q2.png|left|200px|CoQ<s … [[File:Coenzyme Q2.png|left|200px|CoQ2]]'''Coenzyme Q2''' or ubiquinone-2 (CoQ2) is a [[quinone]] derivate composed of a benzoquinone ring with an isoprenoid side chain consisting of two isoprenoid groups, with two methoxy groups, and with one methyl group. In HRR it is used as a Q-mimetic to detect the redox changes of [[coenzyme Q]] at the [[Q-junction]] in conjunction with the [[Q-Module]], since the naturally occurring long-chain coenzyme Q (e.g. CoQ10) is trapped within membrane boundaries. CoQ2 can react both with mitochondrial complexes (e.g. [[CI]], [[CII]] and [[CIII]]) at their quinone-binding sites and with the [[Three-electrode system |detecting electrode]].[[Three-electrode system |detecting electrode]].)
Company of Scientists + ([[File:Company-of-Scientists logo.jpg|left|140px|link=http://www.company-of-scientists.com|Company of Scientists]] The '''Company of Scientists''' evolves as a concept for implementing scientific innovations on the market.)
Unit + ([[File:Count-vs-number.png|right|120px|lin … [[File:Count-vs-number.png|right|120px|link=Elementary entity]]A '''unit''' is defined as 'a single individual thing' in Euclid's ''Elements'' (Book VII). This defines the [[elementary entity]] ''U''''X'' of entity-type ''X'' (thing). The [[International System of Units]] defines the unit as 'simply a particular example of the quantity concerned which is used as a reference'. Then the ''value'' of a quantity ''Q'' is the product of a number ''N'' and a unit ''u''''Q''. The symbols ''U''''X'' and ''u''''Q'' are chosen here with ''U'' and ''u'' for 'unit': ''U''''X'' is the Euclidean or entetic unit ('eunit'), and ''u''''Q'' is the abstract unit ('aunit'). Subscripts ''X'' and ''Q'' for 'entity-type' and 'quantity-type' reflect perhaps even more clearly than words the contrasting meanings of the two fundamental definitions of an entetic versus abstract 'unit'. The term 'unit' with its dual meanings is used and confused in practical language and the scientific literature today. In the elementary entity ''U''''X'', the unit (the 'one') relates to the entity-type ''X'', to the single individual thing (single individual or undivided; the root of the word ''thing'' has the meaning of 'assembly'). The quantity involved in the unit of a single thing is the ''count'', ''N''''X'' = ''N''·''U''''X'' [x]. In contrast to counting, a unit ''u''''Q'' is linked to the measurement of quantities ''Q''''u'' = ''N''·''u''''Q'', such as volume, mass, energy; and these quantities — and hence the units ''u''''Q'' — are abstracted from entity-types, pulled away from the world of real things. The new SI (2019-05-20) has completed this total abstraction of units, from the previous necessity to not only provide a quantitative definition but also a physical realization of a unit in the form of an 'artefact', such as the international prototype (IPK) for the unit kilogram. The new definitions of the base SI units are independent of any physical realization: ''u''''Q'' is separate from ''X''. The classical unit of Euklid is the elementary unit for counting, entirely independent of measuring. Therefore, the quantity [[count]] is unique with respect to two properties: (''1'') in contrast to all other quantities in the metric system, the count depends on quantization of entities ''X''; and (''2'') in the SI, the '''N'''umber 1 is the '''U'''nit of the '''C'''ount of '''entities''' — NUCE.ntrast to all other quantities in the metric system, the count depends on quantization of entities ''X''; and (''2'') in the SI, the '''N'''umber 1 is the '''U'''nit of the '''C'''ount of '''entities''' — NUCE.)
Elementary entity + ([[File:Count-vs-number.png|right|120px|lin … [[File:Count-vs-number.png|right|120px|link=Unit]]An '''elementary entity''' is an [[entity]] of type ''X'', distinguished as a single ''[[unit]]'' of countable objects (''X'' = molecules, cells, organisms, particles, parties, items) or events (''X'' = beats, collisions, emissions, decays, celestial cycles, instances, occurrences, parties). "An elementary entity may be an atom, a molecule, an ion, an electron, any other particle or specified group of particles" ([[Bureau International des Poids et Mesures 2019 The International System of Units (SI) |Bureau International des Poids et Mesures 2019)]]. An elementary entity, therefore, needs to be distinguished from non-countable entities and the general class of entities ''X''. This distinction is emphasized by the term 'elementary' (synonymous with 'elementary entity') with symbol ''U''''X'' and [[unit |elementary unit]] [x].If an object is defined as an assembly of particles (a party of two, a molecule as the assembly of a stoichiometric number of atoms), then the elementary is the assembly but not the assembled particle. A number of defined elementaries ''U''''X'' is a [[count]], ''N''''X'' = ''N''·''U''''X'' [x], where ''N'' is a number, and as such ''N'' is dimensionless, and ''N'' is a ''number'' (stop) and is not 'a number of ..'. Elementaries are added as items to a count. The elementary ''U''''X'' has the [[dimension]] U of the [[count]] ''N''''X''. The elementary ''U''''X'' has the same unit [x] as the count ''N''''X'', or more accurately it gives the count the defining 'counting-unit', which is the 'elementary unit' [x]. From the definition of count as the number (''N'') of elementaries (''U'') of entity type ''X'', it follows that count divided by elementary is a pure number, ''N'' = ''N''''X''·''U''''X''-1. The unit x of a count can neither be the entity ''X'' nor a number. The elementary of type ''X'' defines the identity ''X'' of the elementary ''U''''X'' with the unit 'elementary unit' with symbol [x]. Since a count ''N''''X'' is the number of elementary entities, the elementary ''U''''X'' is not a count (''U''''X'' is not identical with ''N''·''U''''X'').''N''''X'' is the number of elementary entities, the elementary ''U''''X'' is not a count (''U''''X'' is not identical with ''N''·''U''''X'').)
Count + ([[File:Count-vs-number.png|right|120px|lin … [[File:Count-vs-number.png|right|120px|link=Number]]'''Count''' ''N''''X'' is the [[number]] ''N'' of elementary entities of [[entity]]-type ''X''. The single [[elementary entity]] ''U''''X'' is a countable object or event. ''N''''X'' is the number of objects of type ''X'', whereas the term 'entity' and symbol ''X'' are frequently used and understood in dual-message code indicating both (''1'') the entity-type ''X'' and (''2'') a count of ''N''''X'' = 1 x for a single elementary entity ''U''''X''. 'Count' is synonymous with 'number of entities' (number of particles such as molecules, or objects such as cells). Count is one of the most fundamental quantities in all areas of physics to biology, sociology, economy and philosophy, including all perspectives of the statics of countable objects to the dynamics of countable events. The term 'number of entities' can be used in short for 'number of elementary entities', since only elementary entities can be counted, and as long as it is clear from the context, that it is not the number of different entity types that are the object of the count.rom the context, that it is not the number of different entity types that are the object of the count.)
Elementary unit + ([[File:Count-vs-number.png|right|120px|lin … [[File:Count-vs-number.png|right|120px|link=Elementary entity]]The '''elementary unit''' [x] is the unit of a [[count]] ''N''''X'' [x]. The [[International System of Units]] defines the unit of a count as 1. Then the '''N'''umber 1 is the '''U'''nit of the '''C'''ount of '''E'''ntities — NUCE. This causes a number of formal inconsistencies which are resolved by introducing the elementary unit [x] as the abstracted unit of Euclid’s unit, which is an [[elementary entity]] ''U''''X'' [x], and as the unit of Euclid’s number, which is a count ''N''''X'' [x].it of Euclid’s number, which is a count ''N''''X'' [x].)
DORA + ([[File:Dorabadge5.png|150px|right]] The Declaration on Research Assessment '''DORA''' recognizes the need to improve the ways in which researchers and the outputs of scholarly research are evaluated.)
Level flow + ([[File:E.jpg |link=ET capacity]] '''Level … [[File:E.jpg |link=ET capacity]] '''Level flow''' is a [[steady state]] of a system with an input process coupled to an output process (coupled system), in which the output force is zero. ''Clearly, energy must be expended to maintain level flow, even though output is zero'' (Caplan and Essig 1983; referring to zero output force, while output flow may be maximum). force, while output flow may be maximum).)
Noncoupled respiration + ([[File:E.jpg |link=ET capacity]] '''Noncou … [[File:E.jpg |link=ET capacity]] '''Noncoupled respiration''' is distinguished from general (pharmacological or mechanical) [[uncoupled respiration]], to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of [[ET capacity]]. Experimentally uncoupled respiration may fail to yield an estimate of ET capacity, due to inhibition of respiration above optimum uncoupler concentrations or insufficient stimulation by sub-optimal uncoupler concentrations. Optimum uncoupler concentrations for evaluation of (noncoupled) ET capacity require inhibitor titrations ([[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]]; [[Huetter 2004 Biochem J]]; [[Gnaiger 2008 POS]]). Noncoupled respiration is maximum [[electron flow]] in an open-transmembrane proton circuit mode of operation (see [[ET capacity]]).» [[#Is_respiration_uncoupled_-_noncoupled_-_dyscoupled.3F |'''MiPNet article''']][#Is_respiration_uncoupled_-_noncoupled_-_dyscoupled.3F |'''MiPNet article''']])
State 3u + ([[File:E.jpg |link=ET capacity]] Noncouple … [[File:E.jpg |link=ET capacity]] Noncoupled state of [[ET capacity]]. '''State 3u''' (u for uncoupled) has been used frequently in bioenergetics, without sufficient emphasis [[Villani 1998 J Biol Chem|(e.g. Villani et al 1998)]] on the fundamental difference between [[OXPHOS capacity]] (''P'', coupled with an uncoupled contribution; State 3) and noncoupled [[ET capacity]] (''E''; State 3u) ([[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger 2009]]; [[Rasmussen 2000 Mol Cell Biochem|Rasmussen and Rasmussen 2000]]).[[Rasmussen 2000 Mol Cell Biochem|Rasmussen and Rasmussen 2000]]).)
ET capacity + ([[File:E.jpg]] '''T capacity''' is the res … [[File:E.jpg]] '''T capacity''' is the respiratory electron-transfer-pathway capacity ''E'' of mitochondria measured as oxygen consumption in the noncoupled state at optimum [[uncoupler]] concentration. This optimum concentration is obtained by stepwise titration of an established protonophore to induce maximum oxygen flux as the determinant of ET capacity. The experimentally induced noncoupled state at optimum uncoupler concentration is thus distinguished from (''1'') a wide range of uncoupled states at any experimental uncoupler concentration, (''2'') physiological uncoupled states controlled by intrinsic uncoupling (e.g. UCP1 in brown fat), and (''3'') pathological dyscoupled states indicative of mitochondrial injuries or toxic effects of pharmacological or environmental substances. ET capacity in mitochondrial preparations requires the addition of defined fuel substrates to establish an ET-pathway competent state.» [[#Why ET capacity, why not State 3u.3F | '''MiPNet article''']][#Why ET capacity, why not State 3u.3F | '''MiPNet article''']])
EUROMIT + ([[File:EUROMIT.jpg|left|250px]] '''EUROMIT''' is a group based in Europe for organizing '''International Meetings on Mitochondrial Pathology'''.)
Ethanol + ([[File:Ethanol.png|left|80px|Ethanol]] < … [[File:Ethanol.png|left|80px|Ethanol]]<div></div><div>'''Ethanol''' or ethyl alcohol, C2H6O or EtOH, is widely used in the laboratory, particularly as a solvent and cleaning agent. There are different grades of high purity ethanol. Up to a purity of 95.6 % ethanol can be separated from water by destillation. Higher concentrations than 95% require usage of additives that disrupt the azeotrope composition and allow further distillation. Ethanol is qualified as "absolute" if it contains no more than one percent water. Whenever 'ethanol abs.' is mentioned without further specification in published protocols, it refers to ≥ 99 % ethanol a.r. (analytical reagent grade).</div><div></div><div></div>s to ≥ 99 % ethanol a.r. (analytical reagent grade). </div><div> </div><div></div>)
Glutamate-anaplerotic pathway control state + ([[File:G.jpg|left|200px|G]] '''G''': [[Glu … [[File:G.jpg|left|200px|G]] '''G''': [[Glutamate]] is an [[Anaplerotic pathway control state |anaplerotic]] [[Electron-transfer-pathway state |NADH-linked type 4 substrate]] (N). When supplied as the sole fuel substrate in the '''glutamate-anaplerotic pathway control state''', G is transported by the electroneutral glutamate-/OH- exchanger, and is oxidised via mt-[[glutamate dehydrogenase]] in the mitochondrial matrix. The G-pathway plays an important role in [[glutaminolysis]].[[glutaminolysis]].)
GM-pathway control state + ([[File:GM.jpg|left|200px|GM]] '''GM''': [[ … [[File:GM.jpg|left|200px|GM]] '''GM''': [[Glutamate]] & [[Malate]].'''MitoPathway control state:''' [[NADH electron transfer-pathway state]]The '''GM-pathway control state''' (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of [[oxaloacetate]], comparable to the metabolism with acetyl-CoA and citrate synthase.e metabolism with acetyl-CoA and citrate synthase.)
GMS-pathway control state + ([[File:GMS.jpg|left|200px|GMS]]'''GMS''': … [[File:GMS.jpg|left|200px|GMS]]'''GMS''': [[Glutamate]] & [[Malate]] & [[Succinate]].'''MitoPathway control:''' NSTransaminase catalyzes the reaction from oxaloacetate to 2-oxoglutarate, which then establishes a cycle without generation of citrate. OXPHOS is higher with GS (CI&II) compared to GM (CI) or SRot (CII). This documents an additive effect of convergent CI&II electron flow to the Q-junction, with consistent results obtained with permeabilized muscle fibres and isolated mitochondria (Gnaiger 2009).ed muscle fibres and isolated mitochondria (Gnaiger 2009).)
Glutamate + ([[File:Glutamic_acid.jpg|left|100px|Glutam … [[File:Glutamic_acid.jpg|left|100px|Glutamic acid]]'''Glutamic acid''', C5H9NO4, is an amino acid which occurs under physiological conditions mainly as the anion '''glutamate-, G''', with ''p''Ka1 = 2.1, ''p''Ka2 = 4.07 and ''p''Ka3 = 9.47. Glutamate&malate is a substrate combination supporting an N-linked pathway control state, when glutamate is transported into the mt-matrix via the [[glutamate-aspartate carrier]] and reacts with [[oxaloacetate]] in the transaminase reaction to form aspartate and [[oxoglutarate]]. Glutamate as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix by [[glutamate dehydrogenase]] to α-ketoglutarate ([[oxoglutarate|2-oxoglutarate]]), representing the [[glutamate-anaplerotic pathway control state]]. Ammonia (the byproduct of the reaction) passes freely through the mitochondrial membrane.[glutamate-anaplerotic pathway control state]]. Ammonia (the byproduct of the reaction) passes freely through the mitochondrial membrane.)
Glycerophosphate shuttle + ([[File:Gp-shuttle.jpg|left|200px|Gp]] The … [[File:Gp-shuttle.jpg|left|200px|Gp]]The '''glycerophosphate shuttle''' makes cytoplasmic NADH available for mitochondrial oxidative phosphorylation. Cytoplasmic NADH reacts with dihydroxyacetone phosphate catalyzed by cytoplasmic glycerophosphate dehydrogenase. On the outer face of the inner mitochondrial membrane, [[glycerophosphate dehydrogenase complex]] (mitochondrial glycerophosphate dehydrogenase) oxidizes glycerophosphate back to dihydroxyacetone phosphate, a reaction not generating NADH but reducing a flavin prosthesic group. The reduced flavoprotein transfers its reducing equivalents into the [[Q-junction]], thus representing a [[Electron-transfer-pathway state|ET pathway level 3 control state]].[[Electron-transfer-pathway state|ET pathway level 3 control state]].)
Water + ([[File:H2O.jpg|left|60px|Water]] '''Water' … [[File:H2O.jpg|left|60px|Water]]'''Water''', H2O, is widely used in the laboratory, particularly as a solvent and cleaning agent. Chemically pure water is prepared in various grades of purification: double distilled water (ddH2O) versus distilled water (dH2O or [[aqua destillata]], a.d.) and deionized or demineralized water (diH2O) with various combination purification methods. When H2O is mentioned without further specification in published protocols, it is frequently assumed that the standards of each laboratory are applied as to the quality of purified water. Purification is not only to be controlled with respect to salt content and corresponding electrical conductivity (ultra-pure water: 5.5 μS/m due to H+ and OH- ions), but also in terms of microbial contamination. 5.5 μS/m due to H+ and OH- ions), but also in terms of microbial contamination.)
Hydrogen peroxide + ([[File:H2O2.jpg|left|60px|Hydrogen peroxid … [[File:H2O2.jpg|left|60px|Hydrogen peroxide]]'''Hydrogen peroxide''', H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as [[reactive oxygen species]] (ROS). It is formed in various enzyme-catalyzed reactions (''e.g.'', [[superoxide dismutase]]) with the potential to damage cellular molecules and structures. H2O2 is dismutated by [[catalase]] to water and [[oxygen]]. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS. is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS.)
International Mito Patients (IMP) + ([[File:IMP LOGO.JPG|150px]]The '''Internat … [[File:IMP LOGO.JPG|150px]]The '''International Mito Patients''' is a network of national patient organizations involved in mitochondrial disease. Mitochondrial disease is a rare disease with a limited number of patients per country. The national patient organizations which are a member of IMP each are active and powerful in their own countries. By joining forces IMP can represent a large group of patients and as such be their voice on an international level. be their voice on an international level.)
IRDiRC + ([[File:IRDiRC.png|150px]] The Internationa … [[File:IRDiRC.png|150px]] The International Rare Diseases Research Consortium (IRDiRC) teams up researchers and organizations investing in rare diseases research in order to achieve two main objectives by the year 2020, namely to deliver 200 new therapies for rare diseases and means to diagnose most rare diseases. and means to diagnose most rare diseases.)
International Society for Mountain Medicine + ([[File:ISMM.jpg|150px|left|ISMM]]The '''International Society for Mountain Medicine''' is an interdisciplinary society comprising about xx members worldwide. Its purpose is ..)
International Society on Oxygen Transport to Tissue + ([[File:ISOTT LOGO.jpg|200px|left]] The ''' … [[File:ISOTT LOGO.jpg|200px|left]]The '''International Society on Oxygen Transport to Tissue''' is an interdisciplinary society comprising about 250 members worldwide. Its purpose is to further the understanding of all aspects of the processes involved in the transport of oxygen from the air to its ultimate consumption in the cells of the various organs of the body. Founded in 1973, the society has been the leading platform for the presentation of many of the technological and conceptual developments within the field both at the meetings themselves and in the proceedings of the society.ves and in the proceedings of the society.)
Isocitrate + ([[File:Isocitrate.png|left|100px|isocitrat … [[File:Isocitrate.png|left|100px|isocitrate]]'''isocitrate''', C6H5O7-3, is a tricarboxylic acid trianion, intermediate of the [[tricarboxylic acid cycle|TCA cycle]], obtained by isomerization of citrate. The process is catalyzed by [[aconitase]], forming the enzyme-bound intermediate ''cis''-aconitate.[[aconitase]], forming the enzyme-bound intermediate ''cis''-aconitate.)
E-L coupling efficiency + ([[File:J(E-L).jpg|50 px|E-L coupling effic … [[File:J(E-L).jpg|50 px|E-L coupling efficiency]] The '''''E-L'' coupling efficiency''', ''jE-L'' = (''E-L'')/''E'' = 1-''L/E'', is 0.0 at zero coupling (''L''=''E'') and 1.0 at the limit of a fully coupled system (''L''=0). The background state is the [[LEAK respiration|LEAK]] state which is stimulated to flux in the [[electron transfer pathway]] reference state by [[uncoupler]] titration. LEAK states ''L''N or ''L''T may be stimulated first by saturating ADP (rate ''P'' in the OXPHOS state) with subsequent uncoupler titration to the ET state with maximum rate ''E''. The ''E-L'' coupling efficiency is based on measurement of a [[coupling-control ratio]] ([[LEAK-control ratio]], ''L/E''), whereas the thermodynamic or [[ergodynamic efficiency]] of coupling between ATP production (phosphorylation of ADP to ATP) and oxygen consumption is based on measurement of the output/input flux ratio (P»/O2 ratio) and output/input force ratio (Gibbs force of phosphorylation/Gibbs force of oxidation). The [[biochemical coupling efficiency]] expressed as the ''E-L'' coupling efficiency is independent of kinetic control by the ''E-P'' control efficiency, and is equal to the [[P-L control efficiency |''P-L'' control efficiency]] if ''P=E'' as evaluated in a [[coupling-control protocol]].» [[#Biochemical_coupling_efficiency:_from_0_to_.3C1 | '''MiPNet article''']]#Biochemical_coupling_efficiency:_from_0_to_.3C1 | '''MiPNet article''']])
Biochemical coupling efficiency + ([[File:J(E-L).jpg|50 px|link=E-L coupling … [[File:J(E-L).jpg|50 px|link=E-L coupling efficiency |''E-L'' coupling efficiency]] The '''biochemical coupling efficiency''' is the [[E-L coupling efficiency |''E-L'' coupling efficiency]], (''E-L'')/''E'' = 1-''L/E''. This is equivalent to the [[P-L control efficiency |''P-L'' control efficiency]], (''P-L'')/''P'' = 1-''L/P'', only at zero [[E-P excess capacity |''E-P'' excess capacity]], when ''P'' = ''E''). The biochemical coupling efficiency is independent of kinetic control by the phosphorylation system.tic control by the phosphorylation system.)
E-P control efficiency + ([[File:J(E-P).jpg|50 px|E-P control effici … [[File:J(E-P).jpg|50 px|E-P control efficiency]] The '''''E-P'' control efficiency''', ''jE-P'' = (''E-P'')/''E'' = 1-''P/E'', is an expression of the relative limitation of [[OXPHOS capacity]] by the capacity of the [[phosphorylation system]]. It is the normalized ''E-P'' excess capacity. ''jE-P'' = 0.0 when OXPHOS capacity is not limited by the phosphorylation system at zero ''E-P'' excess capacity, ''P''=''E'', when the phosphorylation system does not exert any control over OXPHOS capacity. ''jE-P'' increases with increasing control of the phosphorylation system over OXPHOS capacity. ''jE-P'' = 1 at the limit of zero phosphorylation capacity. The [[OXPHOS]] state of mt-preparations is stimulated to [[electron transfer pathway]] capacity ''E'' by [[uncoupler]] titration, which yields the [[E-P excess capacity |''E-P'' excess capacity]].[[E-P excess capacity |''E-P'' excess capacity]].)
P-L control efficiency + ([[File:J(P-L).jpg|50 px|P-L control effici … [[File:J(P-L).jpg|50 px|P-L control efficiency]] The '''''P-L'' control efficiency''' (''P-L'' flux control efficiency) is defined as ''jP-L'' = (''P-L'')/''P'' = 1-''L/P''. [[OXPHOS capacity]] corrected for [[LEAK respiration]] is the [[P-L net OXPHOS capacity]], ''P-L''. The ''P-L'' control efficiency is the ratio of net to total OXPHOS capacity, which is equal to the biochemical ''E-L'' coupling efficiency, if ''P''=''E''. ''jP-L'' = 1.0 for a fully coupled system (when RCR approaches infinity); ''jP-L'' = 0.0 (RCR=1) for a system with zero respiratory phosphorylation capacity (''P-L''=0) or zero [[E-L coupling efficiency |''E-L'' coupling efficiency]] (''E-L''=0 when ''L''=''P''=''E''). If [[State 3]] is measured at saturating concentrations of ADP and Pi (State 3 = ''P''), then the [[respiratory acceptor control ratio]] RCR equals ''P/L''. Under these conditions, the respiratory control ratio and ''P-L'' control efficiency are related by a hyperbolic function, ''jP-L'' = 1-RCR-1.» [[#Cell ergometry: OXPHOS-control and ET-coupling efficiency |'''MiPNet article''']][#Cell ergometry: OXPHOS-control and ET-coupling efficiency |'''MiPNet article''']])
R-L control efficiency + ([[File:J(R-L).jpg|50 px|R-L ROUTINE-coupli … [[File:J(R-L).jpg|50 px|R-L ROUTINE-coupling efficiency]]The '''''R-L'' control efficiency''', ''jR-L'' = (''R-L'')/''R'' = 1-''L/R'', is the fraction of [[ROUTINE respiration]] coupled to phosphorylation in living cells. ROUTINE respiration is corrected for [[LEAK respiration]] to obtain the [[R-L net ROUTINE capacity |''R-L'' net ROUTINE capacity]]. The flux control efficiency ''jR-L'' is the ''R-L'' net ROUTINE capacity normalized for the reference rate ''R''. The background state is the [[LEAK respiration|LEAK]] state, and the flux control variable is stimulation to ROUTINE respiration by physiologically controlled ATP turnover in living cells.ration by physiologically controlled ATP turnover in living cells.)
Japanese Society of Mitochondrial Research and Medicine + ([[File:J-mit.png|100px|left]]The '''Japane … [[File:J-mit.png|100px|left]]The '''Japanese Society of Mitochondrial Research and Medicine''' (J-mit) was founded to share the latest knowledge on mitochondrial research. J-mit is the biggest Asian society of mitochondrial research and medicine and is a member of [[ASMRM]].[[ASMRM]].)
State 4 + ([[File:L.jpg |link=LEAK respiration]] '''S … [[File:L.jpg |link=LEAK respiration]] '''State 4''' is the [[respiratory state]] obtained in isolated mitochondria after [[State 3]], when added [[ADP]] is phosphorylated maximally to [[ATP]] driven by electron transfer from defined respiratory substrates to O2 ([[Chance 1955 JBC-III|Chance and Williams, 1955]]). State 4 represents [[LEAK respiration]], ''L''T (''L'' for [[LEAK respiration]]; T for ATP), or an overestimation of LEAK respiration if [[ATPase]] activity prevents final accumulation of ATP and maintains a continuous stimulation of respiration by recycled ADP. This can be tested by inhibition of ATP synthase by [[oligomycin]]; ''L''Omy). In the [[LEAK state]] (state of non-phosphorylating resting respiration; static head), oxygen flux is decreased to a minimum (corrected for [[ROX]]), and the [[mt-membrane potential]] is increased to a maximum for a specific substrate or substrate combination.] is increased to a maximum for a specific substrate or substrate combination.)
Static head + ([[File:L.jpg |link=LEAK respiration]] '''S … [[File:L.jpg |link=LEAK respiration]] '''Static head''' is a [[steady state]] of a system with an input process coupled to an output process (coupled system), in which the output force is maximized at constant input or driving force up to a level at which the conjugated output flow is reduced to zero. ''In an incompletely coupled system, energy must be expended to maintain static head, even though the output is zero'' (Caplan and Essig 1983; referring to output flow at maximum output force). [[LEAK respiration]] is a measure of input flow at static head, when the output flow of phosphorylation (ADP->ATP) is zero at maximum phosphorylation potential (Gibbs force of phosphorylation; [[Gnaiger_1993_Hypoxia|Gnaiger 1993a]]). In a completely coupled system, not only the output flux but also the input flux are zero at static head, which then is a state of ''[[ergodynamic equilibrium]]'' ([[Gnaiger_1993_Pure_Appl_Chem |Gnaiger 1993b]]). Whereas the output force is maximum at ergodynamic equilibrium compensating for any given input force, all forces are zero at ''[[thermodynamic equilibrium]]''. Flows are zero at both types of equilibria, hence entropy production or power (power = flow x force) are zero in both cases, i.e. at thermodynamic equilibrium in general, and at ergodynamic equilibrium of a completely coupled system at static head.f a completely coupled system at static head.)
LEAK state without adenylates + ([[File:L.jpg |link=LEAK respiration]] In t … [[File:L.jpg |link=LEAK respiration]] In the '''LEAK state without adenylates''' mitochondrial LEAK respiration, ''L''(n) (n for no adenylates), is measured after addition of substrates, which decreases slowly to the [[LEAK state]] after oxidation of endogenous substrates with no [[adenylates]]. ''L''(n) is distinguished from ''L''(T) and ''L''(Omy).istinguished from ''L''(T) and ''L''(Omy).)
LEAK state with ATP + ([[File:L.jpg |link=LEAK respiration]] The … [[File:L.jpg |link=LEAK respiration]] The '''LEAK state with ATP''' is obtained in mt-preparations without ATPase activity after ADP is maximally phosphorylated to ATP ([[State 4]]; Chance and Williams 1955) or after addition of high ATP in the absence of ADP ([[Gnaiger 2000 Proc Natl Acad Sci U S A |Gnaiger et al 2000]]). Respiration in the LEAK state with ATP, ''L''(T), is distinguished from ''L''(n) and ''L''(Omy).istinguished from ''L''(n) and ''L''(Omy).)
LEAK state with oligomycin + ([[File:L.jpg |link=LEAK respiration]] The … [[File:L.jpg |link=LEAK respiration]] The '''LEAK state with oligomycin''' is a [[LEAK state]] induced by inhibition of ATP synthase by [[oligomycin]]. ADP and ATP may or may not be present. LEAK respiration with oligomycin, ''L''(Omy), is distinguished from ''L''(n) and ''L''(T). distinguished from ''L''(n) and ''L''(T).)
LEAK respiration + ([[File:L.jpg]] '''EAK respiration''' or LE … [[File:L.jpg]] '''EAK respiration''' or LEAK oxygen flux ''L'' compensating for [[proton leak]], [[proton slip]], cation cycling and [[electron leak]], is a dissipative component of respiration which is not available for performing biochemical work and thus related to heat production. LEAK respiration is measured in the LEAK state, in the presence of reducing substrate(s), but absence of ADP - abbreviated as ''L''(n) (theoretically, absence of inorganic phosphate presents an alternative), or after enzymatic inhibition of the [[phosphorylation system]], which can be reached with the use of [[oligomycin]] - abbreviated as ''L''(Omy). The '''LEAK state''' is the non-phosphorylating resting state of intrinsic [[Uncoupler|uncoupled]] or [[Dyscoupled respiration|dyscoupled respiration]] when oxygen flux is maintained mainly to compensate for the proton leak at a high chemiosmotic potential, when ATP synthase is not active. In this non-phosphorylating resting state, the electrochemical proton gradient is increased to a maximum, exerting feedback control by depressing oxygen flux to a level determined mainly by the proton leak and the H+/O2 ratio. In this state of maximum protonmotive force, LEAK respiration, ''L'', is higher than the LEAK component of [[OXPHOS capacity]], ''P''. The conditions for measurement and expression of respiration vary ([[oxygen flux]] in the LEAK state, ''J''O2''L'', or [[oxygen flow]], ''I''O2''L''). If these conditions are defined and remain consistent within a given context, then the simple symbol ''L'' for respiratory rate can be used as a substitute for the more explicit expression for respiratory activity.» [[LEAK respiration#LEAK respiration: concept-linked terminology of respiratory states |'''MiPNet article''']][LEAK respiration#LEAK respiration: concept-linked terminology of respiratory states |'''MiPNet article''']])
Malate-anaplerotic pathway control state + ([[File:M.jpg|left|200px|M]] '''M''': [[Mal … [[File:M.jpg|left|200px|M]] '''M''': [[Malate]] alone does not support respiration of mt-preparations if [[oxaloacetate]] cannot be metabolized further in the absence of a source of acetyl-CoA. Transport of oxaloacetate across the inner mt-membrane is restricted particularly in liver. Mitochondrial citrate and 2-oxoglutarate (α-ketoglutarate) are depleted by antiport with malate. [[Succinate]] is lost from the mitochondria through the dicarboxylate carrier. OXPHOS capacity with malate alone is only 1.3% of that with [[PM |Pyruvate&Malate]] in isolated rat skeletal muscle mitochondria. However, many mammalian and non-mammalian mitochondria have a mt-isoform of NADP+- or NAD(P)<big>+</big>-dependent [[malic enzyme]] (mtME), the latter being particularly active in proliferating cells. Then the [[anaplerotic pathway control state]] with malate alone (aN) supports high respiratory activities comparable to the NADH-linked pathway control states (N) with pyruvate&malate or glutamate&malate substrate combinations ([[PM-pathway control state]], [[GM-pathway control state]]).[GM-pathway control state]]).)
PM-pathway control state + ([[File:M.jpg|left|200px|PM]] '''PM''': [[P … [[File:M.jpg|left|200px|PM]] '''PM''': [[Pyruvate]] & [[Malate]].'''MitoPathway control state:''' [[NADH Electron transfer-pathway state]]Upstream of the NAD-junction, [[Pyruvate]] (P) is oxidatively decarboxylated to acetyl-CoA and CO2, yielding [[NADH]] catalyzed by pyruvate dehydrogenase. [[Malate]] (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase).ate) is formed from isocitrate (isocitrate dehydrogenase).)
MITOEAGLE in MitoGlobal + ([[File:MITOEAGLE-representation.jpg|150px|left]] The objective of the '''MitoEAGLE''' network is to improve our knowledge on mitochondrial function in health and disease related to Evolution, Age, Gender, Lifestyle and Environment.)
MitoKit-CII + ([[File:MITOKIT-CII.jpg|right|180px]]'''Cel … [[File:MITOKIT-CII.jpg|right|180px]]'''Cell permeable prodrugs''', composed of [[MitoKit-CII/Succinate-nv]] and [[MitoKit-CII/Malonate-nv]], stimulates (Snv) or inhibits (Mnanv) mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres, acting on Complex II of the electron transfer system.omplex II of the electron transfer system.)
Rare New England + ([[File:MNE.jpg|left|110px|MNE]] MNE has tr … [[File:MNE.jpg|left|110px|MNE]]MNE has transitioned into RNE (Rare New England). Rare New England is an organization providing access to support groups, gatherings, events and resources for those affected by Rare Disease and living in the New England area.isease and living in the New England area.)
Mitochondria Research Society + ([[File:MRS LOGO.JPG|250px|left]] The '''Mi … [[File:MRS LOGO.JPG|250px|left]]The '''Mitochondria Research Society''' (MRS) is a nonprofit international organization of scientists and physicians. The purpose of MRS is to find a cure for mitochondrial diseases by promoting research on basic science of mitochondria, mitochondrial pathogenesis, prevention, diagnosis and treatment through out the world.nosis and treatment through out the world.)
Malate + ([[File:Malic_acid.jpg|left|100px|Malic aci … [[File:Malic_acid.jpg|left|100px|Malic acid]]'''Malic acid''', C4H6O5, occurs under physiological conditions as the anion '''malate2-, M''', with p''K''a1 = 3.40 and p''K''a2 = 5.20. L-Malate is formed from fumarate in the [[TCA cycle]] in the mitochondrial matrix, where it is the substrate of [[malate dehydrogenase]] oxidized to [[oxaloacetate]]. Malate is also formed in the cytosol. It cannot permeate through the lipid bilayer of membranes and hence requires a carrier ([[dicarboxylate carrier]], [[tricarboxylate carrier]] and 2-oxoglutarate carrier). Malate alone cannot support respiration of [[Mitochondrial preparations|mt-preparations]] from most tissues, since oxaloacetate accumulates in the absence of [[pyruvate]] or [[glutamate]].Malate is a [[NADH electron transfer-pathway state |type N substrate]] (N) required for the [[Fatty acid oxidation pathway control state| FAO-pathway]]. In the presence of [[Malate-anaplerotic pathway control state|anaplerotic pathways]] (''e.g.'', [[Malic enzyme|mitochondrial malic enzyme, mtME]]) the capacity of the FAO-pathway can be overestimated due to a contribution of NADH-linked respiration, F(N) (see [[SUIT-002]]).[[SUIT-002]]).)
Mitochondrial European Education Training + ([[File:Meet.jpg|200px|left]] The '''Mitoch … [[File:Meet.jpg|200px|left]] The '''Mitochondrial European Education Training''' (MEET)MEET is a project started on January 2013. MEET network is composed by a multi-partner project that intends to mobilize the critical mass of expertise, by linking partners from 8 different countries, among which 8 world-leading basic science and clinical centers of excellence, an 1 SME with direct interest in mitochondrial medicine and 3 associated partners that provide for all trainees no-scientific training. MEET is training 11 ESRs and 3 ERs coming from all over the world supervised in their research by 15 mentors and by their collaborators. MEET combine the efforts of leading clinicians with those of more basic oriented groups and will have important implications for the comprehension and treatment of mitochondria-related pathologies.tment of mitochondria-related pathologies.)
MiPMap + ([[File:MiPMap Publication.jpg|left|240px|M … [[File:MiPMap Publication.jpg|left|240px|MiPMap]]The project '''Mitochondrial Physiology Map''' (MiPMap) is initiated to provide an overview of mitochondrial properties in cell types, tissues and species. As part of Bioblast, '''MiPMap''' may be considered as an ''information synthase'' for '''Comparative Mitochondrial Physiology'''. Establishing a comprehensive database will require global input and cooperation.''A comparative database of mitochondrial physiology may provide the key for understanding the functional implications of mitochondrial diversity from mouse to man, and evaluation of altered mitochondrial respiratory control patterns in health and disease'' ([[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger 2009]]).[[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger 2009]]).)
MiR05-Kit + ([[File:MiR05-Kit.jpg|right|180px]] Mitochondrial Respiration Medium - MiR05-Kit, 1 vial; for a final volume of 250 mL)
MitoCanada Foundation + ([[File:Mito Canada logo tag web2.png|200px … [[File:Mito Canada logo tag web2.png|200px|left|MitoCanada]]The '''MitoCanada Foundation'''.The MitoCanada Foundation is Canada’s only not-for-profit organization focused on mitochondrial disease. Since its founding in 2010, MitoCanada has dedicated over $1 million to fund the work of leading Canadian scientists and to support national awareness and support programs.The MitoCanada Foundation is committed to ensuring that those who live with mitochondrial disease are able to enjoy the best possible quality of life until there is a cure.ble quality of life until there is a cure.)
Mitochondrial Research Guild + ([[File:Mito-Reseach-Guild.JPG|200px|left]] … [[File:Mito-Reseach-Guild.JPG|200px|left]]'''The Mitochondrial Research Guild''' is a special interest guild of Seattle Children's Hospital. The guild was founded by a group of families in the Seattle area that are working together to raise awareness, promote research, and improve the quality of medical care that is available to children that are dealing with the devastating and potentially life threatening effects of mitochondrial disease.eatening effects of mitochondrial disease.)
European Bioenergetics Conference + ([[File:Mito-and-Chlora EBEC.png|270px]] '''EBEC''' is a group based in Europe that organizes the '''European Bioenergetics Conference'''.)
MitoAction + ([[File:MitoAction.JPG|230px]]The mission of '''MitoAction''' is to improve quality of life for all who are affected by mitochondrial disorders through support, education and advocacy initiatives.)
MitoFit Preprints + ([[File:MitoFit Preprints.png|left|200px|link=MitoFit Preprints]] '''MitoFit Preprints''' is an Open Access preprint server for mitochondrial physiology and bioenergetics.)
Mitochondrial Medicine Society + ([[File:Mitochondrial Medicine Society.jpg| … [[File:Mitochondrial Medicine Society.jpg|200px|left]]The '''Mitochondrial Medicine Society''' (MMS) was founded in 2000 and represents an international group of physicians, researchers and clinicians working towards the better diagnosis, management, and treatment of mitochondrial diseases., and treatment of mitochondrial diseases.)
Motic Microscope + ([[File:Motic Microscope.jpg|right|180px]]' … [[File:Motic Microscope.jpg|right|180px]]'''Motic Microscope SMZ-171 TLED''': for preparation of permeabilized fibres; compact and light stereo microscope, Greenough optical system, switching power supply for use worldwide (100-240V); including auxiliary ESD objective 2.0X(38.6mm).ding auxiliary ESD objective 2.0X(38.6mm).)
Motive entity + ([[File:Motive entities.png|right|300px|Fro … [[File:Motive entities.png|right|300px|From [[Gnaiger 2020 BEC MitoPathways]]]].A '''motive entity''' ''X''tr is an entity involved in a transformation including spacial transfer. Motive entities (transformants) are expressed in different [[motive unit]]s [MU] depending on the energy transformation under study and the chosen [[format]]. [[Flow]]s are defined as advancement in terms of stoichiometric motive entities per time. Isomorphic [[force]]s are partial derivatives of Gibbs energy per advancement. Ions carrying a positive charge (cations) or negative charge (anions) may be considered as a paradigm of motive entities, since Faraday did not coin but introduced the term 'ion', which is old Greek for 'going' — advancing to the cathode or anode and thus generating an electric [[current]].current]].)
SUIT-033 NADH mt D081 + ([[File:Mt;1D.1;2PGM;3D2.5;4Anox;5Myx;6Reox.png|400px]])
SUIT-033 O2 mt D110 + ([[File:Mt;1D.1;2PGM;3D2.5;4Myx.png|400 px]])
SUIT-034 NADH mt D082 + ([[File:Mt;1D.1;2PGM;3D2.5;4U;5Anox;6Myx;7Reox.png|400px]])
SUIT-034 O2 mt D111 + ([[File:Mt;1D.1;2PGM;3D2.5;4U;5Myx.png|400px]])
SUIT-032 O2 mt D109 + ([[File:Mt;1PGM;2D;3Myx.png|300 px]])
SUIT-001 O2 mt D001 + ([[File:Mt;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd .png|400px|SUIT-RP1]])
SUIT-031 Q mt D072 + ([[File:Mt;1Q2;1PM;2D;3S;4Rot;5U;6Anox;7Ama.png|400px]])
The North American Mitochondrial Disease Consortium + ([[File:NAMDC.JPG|200px|left]] '''The North … [[File:NAMDC.JPG|200px|left]]'''The North American Mitochondrial Disease Consortium (NAMDC)''' was established to create a network of all clinicians and clinical investigators in North America (US and Canada, with the hope of including Mexico in the future) who follow sizeable numbers of patients with mitochondrial diseases and are involved or interested in mitochondrial research. The NAMDC has created a clinical registry for patients, in the hopes of standardizing diagnostic criteria, collecting important standardized information on patients, and facilitating the participation of patients in research on mitochondrial diseases.For the study of any rare disease, the collection of specimens is a major challenge. The '''NAMDC''' is establishing a repository for specimens and DNA from patients with mitochondrial diseases, in order to make materials easily available to consortium researchers.Finally, the '''NAMDC''' will conduct clinical trials and other kinds of research. The consortium makes biostatisticians, data management experts, and specialists in clinical research available to participating physicians, so that experiments conducted through the NAMDC can make the most efficient and innovative use of the generous participation of patients.of the generous participation of patients.)
Neurocon + ([[File:Neurocon LOGO.JPG|200px|left]] '''Neurocon''' is an Indian society organizing international conferences on neurodegenerative and neurodevelopmental diseases.)
Oroboros O2k-technology + ([[File:NextGen-O2k All-in-one 2023.jpg|300 … [[File:NextGen-O2k All-in-one 2023.jpg|300px|right|NextGen-O2k all-in-one]]The '''Oroboros O2k-technology''' provides modular systems for [[high-resolution respirometry]] (HRR) for mitochondria and cell research. Oroboros delivers the O2k-technology for high-resolution respirometry in mitochondria and cell research. The O2k-tecnology allows the measurement of respiration at controlled oxygen levels, combined with redox biology (NADH and CoQ), ROS production, mitochondrial membrane potential, ATP production, Ca2+, or pH. HRR expands to HRPB: High-Resolution PhotoBiology. Small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to living cells and tissues slices. The modular O2k-concept is supported by [[DatLab |DatLab]], with high flexibility for extension by add-on [https://www.oroboros.at/index.php/product-category/products/o2k-modules/ O2k-Modules]. All O2k-Modules are supported by the [[NextGen-O2k]]. The [https://www.oroboros.at/index.php/product/q-module/ O2k-Q-Module] and the [https://www.oroboros.at/index.php/product/nadh-module/ O2k-NADH-Module] are exclusively supported by the NextGen-O2k, whereas the O2k (Series-J) provides the basis for all other HRR application but cannot be upgraded to the [https://www.oroboros.at/index.php/product/nextgen-o2k-redox/ NextGen-O2k Redox]. The globally tested and trusted high-resolution O2k-technology prioritizes both quality and scientific research output in the field of mitochondrial physiology and pathology, extended to PhotoBiology.al physiology and pathology, extended to PhotoBiology.)
O-ring\Viton\12.5x1 mm + ([[File:O-ringViton12.5x1 mm.jpg|right|180px]]'''O-ring\[[Viton]]\12.5x1 mm''', for PVDF or PEEK O2k-Stoppers (2-mL O2k-Chamber), box of 8 as spares.)
Superoxide + ([[File:O2-.jpg|left|60px|Superoxide anion] … [[File:O2-.jpg|left|60px|Superoxide anion]]'''Superoxide anion''', O2•-, is a free radical formed in a one-electron reduction of molecular [[oxygen]] (red bullet in the figure), yielding a negatively charged molecule with a single unpaired electron (blue bullet on the left). It is highly reactive with organic compounds, and its intracellular concentration is kept under control by [[superoxide dismutase]].superoxide dismutase]].)
Oxygen + ([[File:O2.jpg|left|60px|Dioxygen]] '''Mole … [[File:O2.jpg|left|60px|Dioxygen]]'''Molecular oxygen''', O2 or '''dioxygen''', has two atoms of oxygen, O, which is the chemical element with atomic number 8. The relative molecular mass of O2, ''M''r,O2, is 32 (or 31.9988). The element O has 8 protons, 8 neutrons and 8 electrons. In the figure, the two electrons in the first electron shell are not shown. Of the six electrons in the outer shell (blue bullets), one electron from each of the two atoms is shared in O2 forming the covalent bond, and one electron in each atom is unpaired.sub>2 forming the covalent bond, and one electron in each atom is unpaired.)
O2k-chamber + ([[File:O2k-Chamber.jpg|right|180px]] '''O2 … [[File:O2k-Chamber.jpg|right|180px]]'''O2k-Chamber''': Duran® glass polished, with standard operation volumes (''V'') of 2.0 mL or 0.5 mL (small chamber volume in the [[O2k-sV-Module]], 12 mm inner diameter). The optical properties of Duran® allow application of fluorometric sensors ([http://www.duran-group.com/en/about-duran/duran-properties/optical-properties-of-duran.html Duran® optical properties]).f-duran.html Duran® optical properties]).)
Open Science + ([[File:Open Access logo.png |20px |left]] … [[File:Open Access logo.png |20px |left]] Building on the essential principles of academic freedom, research integrity and scientific excellence, '''open science''' sets a new paradigm that integrates into the scientific enterprise practices for reproducibility, transparency, sharing and collaboration resulting from the increased opening of scientific contents, tools and processes. Open science is defined as an inclusive construct that combines various movements and practices aiming to make multilingual scientific knowledge openly available, accessible and reusable for everyone, to increase scientific collaborations and sharing of information for the benefits of science and society, and to open the processes of scientific knowledge creation, evaluation and communication to societal actors beyond the traditional scientific community. It comprises all scientific disciplines and aspects of scholarly practices, including basic and applied sciences, natural and social sciences and the humanities, and it builds on the following key pillars: open scientific knowledge, open science infrastructures, science communication, open engagement of societal actors and open dialogue with other knowledge systems.pen dialogue with other knowledge systems.)
Open Access + ([[File:Open Access logo.png |20px |left]] … [[File:Open Access logo.png |20px |left]] '''Open Access''' (OA) academic articles comprise all different forms of published research that are distributed online, free of charge and with an open license to facilitate the distribution and reuse. The open access repositories serve as the perfect vehicle to transmit free knowledge, including but not limited to peer-reviewed and non-peer-reviewed academic journal articles, conference papers, theses, book chapters and monographs. Driven by the problems of social inequality caused by restricting access to academic research, the Open Access movement changes the funding system of published literature allowing for more readers and thus increased access to scientific knowledge, as well as addressing the economic challenges and unsustainability of academic publishing. In addition to being free to read (''gratis''), open access articles may also be free to use (''libre'') where the copyright is held by the authors and not the publisher.Definition by the [[Directory of Open Access Journals]] (DOAJ): "We define these as journals where the copyright holder of a scholarly work grants usage rights to others using an open license (Creative Commons or equivalent) allowing for immediate free access to the work and permitting any user to read, download, copy, distribute, print, search, or link to the full texts of articles, crawl them for indexing, pass them as data to software, or use them for any other lawful purpose."or use them for any other lawful purpose.")
O2k-USB Flash Drive + ([[File:Oroboros-USB-flash-drive.JPG|right|120px]] The '''O2k-USB Flash Drive''' is a component of the [[Oroboros O2k]] containing: [[DatLab]], O2k-Manual, O2k-Protocols, O2k-Publications, and info on O2k-Workshops.)
Oxaloacetate + ([[File:Oxaloacetic_acid.jpg|left|100px|Oxa … [[File:Oxaloacetic_acid.jpg|left|100px|Oxaloacetic acid]]'''Oxaloacetic acid''', C4H4O5, occurs under physiological conditions as the anion '''oxaloacetate2-, Oa'''. Oxaloacetate is formed from malate by [[Malate dehydrogenase|MDH]]. Oa reacts with acetyl-CoA through [[citrate synthase]] to form citrate, or with [[glutamate]] through transaminase to form [[oxoglutarate]] and aspartate. Oa transport is restricted across the inner [[mitochondrial|mt]]-membrane of various tissues. Oa is a potent inhibitor of [[succinate dehydrogenase]].[[succinate dehydrogenase]].)
Normoxia + ([[File:Oxia terms.png|right|300px|link=htt … [[File:Oxia terms.png|right|300px|link=https://www.oroboros.at/index.php/product/oxia/|Oxia]]'''Normoxia''' is a reference state, frequently considered as air-level oxygen pressure at sea level (c. 20 kPa in water vapor saturated air) as environmental normoxia. Intracellular tissue normoxia is variable between organisms and tissues, and intracellular oxygen pressure is frequently well below air-level ''p''O2 as a result of cellular (mainly mitochondrial) oxygen consumption and oxygen gradients along the respiratory cascade. Oxygen pressure drops from ambient normoxia of 20 kPa to alveolar normoxia of 13 kPa, while extracellular normoxia may be as low as 1 to 5 kPa in solid organs such as heart, brain, kidney and liver. Pericellular ''p''O2 of cells growing in monolayer cell cultures may be [[hypoxic]] compared to tissue normoxia when grown in ambient normoxia (95 % air and 5 % CO2) and a high layer of culture medium causing oxygen diffusion limitation at high respiratory activity, but pericellular ''p''O2 may be effectively [[hyperoxic]] in cells with low respiratory rate with a thin layer of culture medium (<2 mm). Intracellular oxygen levels in well-stirred suspended small cells (5 - 7 mm diameter; endothelial cells, fibroblasts) are close to ambient ''p''O2 of the incubation medium, such that matching the experimental intracellular ''p''O2 to the level of intracellular tissue normoxia requires lowering the ambient ''p''O2 of the medium to avoid hyperoxia.O2 to the level of intracellular tissue normoxia requires lowering the ambient ''p''O2 of the medium to avoid hyperoxia.)
Oxidative phosphorylation + ([[File:P.jpg |link=OXPHOS capacity]] '''Ox … [[File:P.jpg |link=OXPHOS capacity]] '''Oxidative phosphorylation''' (OXPHOS) is the oxidation of reduced fuel substrates by electron transfer to oxygen, chemiosmotically coupled to the phosphorylation of [[ADP]] to [[ATP]] (P») and accompanied by an intrinsically uncoupled component of respiration. The OXPHOS state of respiration provides a measure of [[OXPHOS capacity]] (''P''), which is frequently corrected for [[residual oxygen consumption]] (ROX).[residual oxygen consumption]] (ROX).)
State 3 + ([[File:P.jpg |link=OXPHOS capacity]] '''St … [[File:P.jpg |link=OXPHOS capacity]] '''State 3''' respiration is the ADP stimulated respiration of isolated coupled mitochondria in the presence of high ADP and Pi concentrations, supported by a defined substrate or substrate combination at saturating oxygen levels [[Chance_1955_JBC-III|(Chance and Williams, 1955]]). State 3 respiration can also be induced in [[Permeabilized tissue or cells|permeabilized cells]], including permeabilized tissue preparations and tissue homogenates. ADP concentrations applied in State 3 are not necessarily saturating, whereas [[OXPHOS capacity]] is measured at saturating concentrations of ADP and Pi (OXPHOS state). For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the [[P/O ratio]] in State 3→4 (D→T) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before [[State 4]] is obtained ([[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger et al 2000]]; [[Puchowicz_2004_Mitochondrion|Puchowicz et al 2004]]). Respiration in the OXPHOS state or in State 3 is well [[coupled respiration|coupled]], and partially [[uncoupled respiration|uncoupled]] (physiological) or partially [[dyscoupled respiration|dyscoupled]] (pathological). A high [[mt-membrane potential]] provides the driving force for oxidative phosphorylation, to phosphorylate ADP to ATP and to transport ADP and ATP across the mitochondrial inner membrane (mtIM) through the [[adenine nucleotide translocase]] (ANT). The mt-membrane potential is reduced, however, in comparison to the [[LEAK state]] of respiration, whereas the cytochromes are in a more oxidized redox state.ation, whereas the cytochromes are in a more oxidized redox state.)
OXPHOS capacity + ([[File:P.jpg]] '''OXPHOS capacity''' ''P'' … [[File:P.jpg]] '''OXPHOS capacity''' ''P'' is the respiratory capacity of mitochondria in the ADP-activated state of [[oxidative phosphorylation]], at saturating concentrations of [[ADP]] and inorganic phosphate (which may not be the case in [[State 3]]), oxygen, and defined reduced CHNO-fuel substrates. and defined reduced CHNO-fuel substrates.)
PGM-pathway control state + ([[File:PGM.jpg|left|200px|PGM]] '''PGM''': … [[File:PGM.jpg|left|200px|PGM]] '''PGM''': [[Pyruvate]] & [[Glutamate]] & [[Malate]].'''MitoPathway control state:''' [[NADH electron transfer-pathway state]][[Pyruvate]] (P) is oxidatively decarboxylated to acetyl-CoA and CO2, yielding [[NADH]] catalyzed by pyruvate dehydrogenase. [[Malate]] (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). Glutamate&malate is a substrate combination supporting an N-linked pathway control state, when glutamate is transported into the mt-matrix via the [[glutamate-aspartate carrier]] and reacts with [[oxaloacetate]] in the [[transaminase]] reaction to form [[aspartate]] and [[oxoglutarate]]. Glutamate as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix by [[glutamate dehydrogenase]] to α-ketoglutarate ([[oxoglutarate | 2-oxoglutarate]]), representing the [[glutamate-anaplerotic pathway control state]]. 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase.tate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase.)
PGMS-pathway control state + ([[File:PGMS.png|left|200px|PGMS]] '''PGMS' … [[File:PGMS.png|left|200px|PGMS]] '''PGMS''': [[Pyruvate]] & [[Glutamate]] & [[Malate]] & [[Succinate]].'''MitoPathway control state:''' [[NS|NS-pathway control state]]2-oxoglutarate is produced through the citric acid cycle from citrate by isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase. If the 2-oxoglutarate carrier does not outcompete these sources of 2-oxoglutarate, then the TCA cycle operates in full circle with external pyruvate&malate&glutamate&succinatercle with external pyruvate&malate&glutamate&succinate)
O2k-pH ISE-Module + ([[File:PH new.jpg|right|180px]]'''O2k-pH ISE-Module''': two pH electrodes and reference electrodes and accessories)
PMS-pathway control state + ([[File:PMS.jpg|left|200px|PMS]]'''PMS''': … [[File:PMS.jpg|left|200px|PMS]]'''PMS''': [[Pyruvate]] & [[Malate]] & [[Succinate]].'''MitoPathway control:''' CI&II[[Pyruvate]] (P) is oxidatively decarboxylated to acetyl-CoA and CO2, yielding [[NADH]] catalyzed by pyruvate dehydrogenase. [[Malate]] (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). This documents an additive effect of convergent CI&II electron flow to the Q-junction, with consistent results obtained with permeabilized muscle fibres and isolated mitochondria (Gnaiger 2009). permeabilized muscle fibres and isolated mitochondria (Gnaiger 2009).)
O-ring\Viton\8x1 mm + ([[File:POS O-ring for sensor head or POS mounting tool.jpg|right|180px]]'''O-ring\Viton\8x1 mm''': for [[OroboPOS]] sensor head. Replaces the O-ring\Viton\9x1 mm)
O-ring\Viton\6x1 mm + ([[File:POS O-ring for sensor head or POS mounting tool.jpg|right|180px]]'''O-ring\Viton\6x1 mm''' for [[POS-Mounting Tool]].)
POS-Membrane Ring + ([[File:POS membrane holder ring.jpg|right|180px|link=]]'''POS-Membrane Ring''', [[PEEK]], holds the membrane against the inner O-ring on the POS housing.)
SUIT-002 O2 pfi D006 + ([[File:Pfi;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px|SUIT-RP2]])
SUIT-001 O2 pfi D002 + ([[File:Pfi;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]])
Phosphorylation pathway + ([[File:Phosphorylation system.jpg|thumb|le … [[File:Phosphorylation system.jpg|thumb|left|250px|From Gnaiger 2014 MitoPathways]]The '''phosphorylation pathway''' (phosphorylation system) is the functional unit utilizing the protonmotive force to phosphorylate ADP (D) to ATP (T), and may be defined more specifically as the '''P»-system'''. The P»-system consists of [[adenine nucleotide translocase]], [[phosphate carrier]], and [[ATP synthase]]. Mitochondrial [[adenylate kinase]], mt-[[creatine kinase]] and mt-[[hexokinase]] constitute extended components of the P»-system, controlling local AMP and ADP concentrations and forming [[metabolic channel]]s. Since substrate-level phosphorylation is involved in the TCA-cycle, the P»-system includes [[succinyl-CoA ligase]] (GDP to GTP or ADP to ATP).ccinyl-CoA ligase]] (GDP to GTP or ADP to ATP).)
Pipette\Plastic\1 ml ungraded + ([[File:Pipette Plastic 1 ml-ungraded.JPG|180px|right]]'''Pipette\Plastic\1 mL ungraded''', for filling electrolyte into the reservoir of the [[OroboPOS]].)
Power O2k Numbers + ([[File:Power O2k Numbers.JPG|right|180px]]'''Power O2k Numbers''': Single number to label the O2k in a Power O2k-Lab.)
Proline + ([[File:Proline.png|left|100px|Proline]] '' … [[File:Proline.png|left|100px|Proline]]'''Proline''' (Pro), C5H9NO2, is an amino acid which occurs under physiological conditions mainly in the nonpolar form, with ''p''Ka1 = 1.99 ''p''Ka2 = 10.96.Proline is an [[anaplerotic]] substrate that supports both the proline pathway control state and the [[glutamate-anaplerotic pathway control state]]. Proline is used as a single substrate or in combination with carbohydrate-derived metabolites in mitochondria particularly of flight muscle of many (but not all) insects. Proline is oxidized to delta-1-pyrroline-5-carboxylate by the [[mtIM]] L-proline:quinone oxidoreductase ([[proline dehydrogenase]], ProDH), with reduction of FAD to FADH2 and direct entry into the [[Q-junction]]. delta-1-pyrroline-5-carboxylate is converted to [[glutamate]] by 1-pyrroline-5-carboxylate dehydrogenase.[[glutamate]] by 1-pyrroline-5-carboxylate dehydrogenase.)
Pyruvate + ([[File:Pyruvic_acid.jpg|left|80px|Pyruvic … [[File:Pyruvic_acid.jpg|left|80px|Pyruvic acid]]'''Pyruvic acid''', C3H4O3, is an alpha-keto monocarboxylic acid which occurs under physiological conditions mainly as the anion '''pyruvate-, P''', with ''p''Ka = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of [[lactate dehydrogenase]]. Pyruvate enters the mitochondrial matrix via a specific low ''K''m' H+/monocarboxylate cotransporter known as the [[pyruvate carrier]]. Similarly, the plasma membrane of many cell types has H+/monocarboxylate cotransporter activity and pyruvate can thus be added as a substrate to living cells. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by [[pyruvate dehydrogenase]] and yields [[acetyl-CoA]]. Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by [[cyanide]]. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]].[[hydrogen peroxide]].)
ROUTINE respiration + ([[File:R.jpg]] In the living cell, '''ROUT … [[File:R.jpg]] In the living cell, '''ROUTINE respiration''' (''R'') or ROUTINE activity in the physiological coupling state is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic [[uncoupling]] and pathological [[dyscoupling]]). The conditions for measurement and expression of respiration vary ([[oxygen flux]] in state ''R'', ''J''O2''R'' or [[oxygen flow]] in state ''R'', ''I''O2''R''). If these conditions are defined and remain consistent within a given context, then the simple symbol ''R'' for respiratory state can be used to substitute the more explicit expression for respiratory activity. ''R'' and growth of cells is supported by exogenous substrates in culture media. In media without energy substrates, ''R'' depends on endogenous substrates. ''R'' cannot be measured in [[permeabilized cells]] or [[isolated mitochondria]]. ''R'' is corrected for [[residual oxygen consumption]] (ROX), whereas ''R''´ is the uncorrected apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX. apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX.)
State 2 + ([[File:ROX.jpg |link=Residual oxygen consu … [[File:ROX.jpg |link=Residual oxygen consumption]] Substrate limited state of [[residual oxygen consumption]], after addition of [[ADP]] to isolated mitochondria suspended in mitochondrial respiration medium in the absence of reduced substrates (ROXD). Residual endogenous substrates are oxidized during a transient stimulation of oxygen flux by ADP. The peak – supported by endogenous substrates – is, therefore, a pre-steady state phenomenon preceding State 2. Subsequently oxygen flux declines to a low level (or zero) at the steady '''State 2''' ([[Chance_1955_JBC-III|Chance and Williams 1955]]). ADP concentration (D) remains high during ROXD.concentration (D) remains high during ROXD.)
Residual oxygen consumption + ([[File:ROX.jpg|100px|link=https://wiki.oro … [[File:ROX.jpg|100px|link=https://wiki.oroboros.at/images/3/30/ROX.jpg]] '''Residual oxygen consumption''' ''Rox'' — respiration in the ROX state — is due to oxidative side reactions remaining after inhibition of the [[electron transfer pathway]] (ET pathway) in [[mitochondrial preparation]]s or living cells. Different conditions designated as ROX states (different combinations of inhibitors of CI, CII, CIII and CIV) may result in consistent or significantly different levels of oxygen consumption. Hence the best quantitative estimate of ''Rox'' has to be carefully evaluated. Mitochondrial respiration is frequently corrected for ''Rox'' as the [[baseline state]]. Then, total [[ROUTINE]], [[LEAK respiration]], [[OXPHOS]] or [[Electron transfer pathway |ET]] (''R'', ''L'', ''P'' and ''E'') respiration are distinguished from the corresponding ''Rox''-corrected, mitochondrial (ET-pathway linked) fluxes: ''R''(mt), ''L''(mt), ''P''(mt) and ''E''(mt). Alternatively, ''R'', ''L'', ''P'' and ''E'' are defined as ''Rox''-corrected rates, in contrast to total rates ''R''´, ''L''´, ''P''´ and ''E''´. When expressing ''Rox'' as a fraction of ET capacity ([[flux control ratio]]), total flux ''E''´ (not corrected for ''Rox''), should be taken as the reference. ''Rox'' may be related to, but is of course different from [[ROS]] production.In previous editions, (including [[Gnaiger 2020 BEC MitoPathways]]), the [[REN]] state was not distinguished from the ROX state. However, in novel applications (Q-Module and NADH-Module), a distinction of these states is necessary. Care must be taken when assuming ''Ren'' as a substitute of ''Rox'' correction of mitochondrial respiration.' correction of mitochondrial respiration.)
Ambiguity crisis + ([[File:Rabbit or duck.jpg|right|300px|thum … [[File:Rabbit or duck.jpg|right|300px|thumb|'''Graphical ambiguity:''' ''Fliegende Blätter'' (1892-10-23): Perception versus interpretation (Ludwig Wittgenstein) or paradigm shift (Thomas Kuhn)]]The '''ambiguity crisis''' is a contemporary crisis comparable to the credibility or [[reproducibility crisis]] in the biomedical sciences. The term 'crisis' is rooted etymologically in the Greek word ''krinein'': meaning to 'separate, decide, judge'. In this sense, science and communication in general are a continuous crisis at the edge of separating clarity or certainty from confusing double meaning, or obscure 'alchemical' gibberish, or even fake-news. Reproducibility relates to the condition of repeating and confirming calculations or experiments presented in a published resource. While ambiguity is linked to relevant issues of reproducibility, it extends to the communications space of terminological and graphical representations of concepts. Type 1 ambiguities are the inevitable consequence of conceptual evolution, in the process of which ambiguities are replaced by experimentally and theoretically supported paradigm shifts to clear-cut theorems. In contrast, type 2 ambiguities are traced in publications that reflect merely a disregard and ignorance of established concepts without an attempt to justify the inherent deviations from high-quality science. There are many shades of grey between these types of ambiguity. of grey between these types of ambiguity.)
Residual endogenous substrates + ([[File:Ren.png|100px|link=https://wiki.oro … [[File:Ren.png|100px|link=https://wiki.oroboros.at/index.php/File:Ren.png]] Oxygen consumption due to '''residual endogenous substrates'''. ''Ren'' is the respiration in the REN state. It is due to oxidative reactions in [[mt-preparation]]s incubated without addition of fuel substrates in the absence or presence of ADP (in the presence of ADP to stimulate the consumption of endogenous fuel substrates: [[State 2]]). ''Ren'' values may be used as technical replicates when obtained from the same mt-preparation in different protocols. ''Ren'' may be higher than ''Rox''. Correspondingly, Q and NAD are not fully oxidized in the REN state compared to the ROX state. In previous editions (including [[Gnaiger 2020 BEC MitoPathways]]), the REN state was not distinguished from the [[ROX]] state. However, in novel applications (Q-Module and NADH-Module), a distinction of these states is necessary. Care must be taken when assuming ''Ren'' as a substitute of ''Rox'' correction of mitochondrial respiration.' correction of mitochondrial respiration.)
Society for Heart and Vascular Metabolism + ([[File:SHVM.png|100px|left]]The '''Society … [[File:SHVM.png|100px|left]]The '''Society for Heart and Vascular Metabolism''' (SHVM) The Society for Heart and Vascular Metabolism was founded in 2001, with the intent of providing a forum for the free exchange of ideas by a group of investigators that had a special interest in the multiple roles of intermediary metabolism in the cardiovascular system. An important aim of the Society is to foster interactions between young investigators and senior scientists and our meetings are deliberately designed to maximize these interactions. There is growing recognition across many areas of scientific investigation and in the cardiovascular arena of the importance of metabolic homeostasis. The Society for Heart and Vascular Metabolism intends to remain at the vanguard of this rapidly expanding area.e vanguard of this rapidly expanding area.)
Society for Mitochondrial Research and Medicine - India + ([[File:SMRM.JPG|150px|left]]The Society fo … [[File:SMRM.JPG|150px|left]]The Society for '''Mitochondria Research and Medicine - India''' (SMRM-India) is a nonprofit organization of scientists, clinicians and academicians. The purpose of SMRM is to foster research on basic science of mitochondria, mitochondrial pathogenesis, prevention, diagnosis and treatment through out India and abroad.nd treatment through out India and abroad.)
Serbian Society for Mitochondrial and Free Radical Physiology + ([[File:SSMFRP.jpg|left|200px]] The '''Serb … [[File:SSMFRP.jpg|left|200px]] The '''Serbian Society for Mitochondrial and Free Radical Physiology (SSMFRP)''' was established in 2008 as a national Society and has 150 members who gather research in the fields of molecular biology, biochemistry, medicine, chemistry, agriculture, physics and other related disciplines. The SSMFRP was founded as a '''voluntary non-governmental and non-profit association''' for researchers whose goal is to support the creative improvement of scientific knowledge about the '''physiology of mitochondria and free radicals''', support for the development of modern research approaches and integration of fundamental research in order to better understand the role of free radicals in pathophysiological states, as well as promoting scientific knowledge in the country and abroad.tific knowledge in the country and abroad.)
SUIT-003 pH ce D067 + ([[File:SUIT-003 O2 ce D067 diagram.png|350px]])
SUIT-026 AmR mt D064 + ([[File:SUIT-026 AmR mt D064.png|400px]])
SUIT-026 AmR mt D077 + ([[File:SUIT-026 AmR mt D064.png|400px]])
F-junction + ([[File:SUIT-catg F.jpg|right|300px|F-junct … [[File:SUIT-catg F.jpg|right|300px|F-junction]]The '''F-junction''' is a junction for [[convergent electron flow]] in the [[electron transfer pathway]] (ET-pathway) from fatty acids through [[fatty acyl CoA dehydrogenase]] (reduced form [[FADH2]]) to [[electron transferring flavoprotein]] (CETF), and further transfer through the [[Q-junction]] to [[Complex III]] (CIII). The concept of the F-junction and [[N-junction]] provides a basis for defining [[categories of SUIT protocols]]. Fatty acid oxidation, in the [[F-pathway control state]], not only depends on electron transfer through the F-junction (which is typically rate-limiting) but simultaneously generates NADH and thus depends on N-junction throughput. Hence FAO can be inhibited completely by inhibition of Complex I (CI). In addition and independent of this source of NADH, the N-junction substrate malate is required as a co-substrate for FAO in mt-preparations, since accumulation of AcetylCoA inhibits FAO in the absence of malate. Malate is oxidized in a reaction catalyzed by malate dehydrogenase to oxaloacetate (yielding NADH), which then stimulates the entry of AcetylCoA into the TCA cycle catalyzed by citrate synthase.e TCA cycle catalyzed by citrate synthase.)
Fatty acid oxidation pathway control state + ([[File:SUIT-catg F.jpg|right|300px|F-junct … [[File:SUIT-catg F.jpg|right|300px|F-junction]]In the '''fatty acid oxidation pathway control state''' (F- or FAO-pathway), one or several fatty acids are supplied to feed electrons into the [[F-junction]] through fatty acyl CoA dehydrogenase (reduced form [[FADH2]]), to [[electron transferring flavoprotein]] (CETF), and further through the [[Q-junction]] to [[Complex III]] (CIII). FAO not only depends on electron transfer through the F-junction (which is typically rate-limiting relative to the N-pathway branch), but simultaneously generates FADH2 and NADH and thus depends on [[N-junction]] throughput. Hence FAO can be inhibited completely by inhibition of [[Complex I]] (CI). In addition and independent of this source of NADH, the type N substrate malate is required at low concentration (0.1 mM) as a co-substrate for FAO in mt-preparations, since accumulation of Acetyl-CoA inhibits FAO in the absence of malate. Malate is oxidized in a reaction catalyzed by malate dehydrogenase to oxaloacetate (yielding NADH), which then stimulates the entry of Acetyl-CoA into the TCA cycle catalyzed by citrate synthase. Peroxysomal ''β''-oxidation carries out few ''β''-oxidation cycles, thus shortening very-long-chain fatty acids (>C20) for entry into mitochondrial ''β''-oxidation. Oxygen consumption by peroxisomal [[acyl-CoA oxidase]] is considered as [[residual oxygen consumption]] rather than cell respiration.esidual oxygen consumption]] rather than cell respiration.)
FN + ([[File:SUIT-catg FN.jpg|right|300px|F-junc … [[File:SUIT-catg FN.jpg|right|300px|F-junction]]FN is induced in mt-preparations by addition of [[NADH]]-generating substrates ([[N-pathway control state]], or CI-linked pathway control) in combination with one or several fatty acids, which are supplied to feed electrons into the [[F-junction]] through [[fatty acyl CoA dehydrogenase]] (reduced form [[FADH2]]), to [[electron transferring flavoprotein]] (CETF), and further through the [[Q-junction]] to [[Complex III]] (CIII). FAO not only depends on electron transfer through the F-junction (which is typically rate-limiting), but simultaneously generates FADH2 and NADH and thus depends on [[N-junction]] throughput. Hence FAO can be inhibited completely by inhibition of [[Complex I]] (CI). This physiological substrate combination is required for partial reconstitution of [[TCA cycle]] function and convergent electron-input into the [[Q-junction]], to compensate for metabolite depletion into the incubation medium. FS in combination exerts an [[additive effect of convergent electron flow]] in most types of mitochondria.[[additive effect of convergent electron flow]] in most types of mitochondria.)
FNS + ([[File:SUIT-catg FNS.jpg|right|300px|F-jun … [[File:SUIT-catg FNS.jpg|right|300px|F-junction]]FNS is induced in mt-preparations by addition of [[NADH]]-generating substrates ([[N-pathway control state]], or CI-linked pathway control) in combination with [[succinate]] ([[S-pathway control state]]; S- or CII-linked) and one or several fatty acids, which are supplied to feed electrons into the [[F-junction]] through [[fatty acyl CoA dehydrogenase]] (reduced form [[FADH2]]), to [[electron transferring flavoprotein]] (CETF), and further through the [[Q-junction]] to [[Complex III]] (CIII). FAO not only depends on electron transfer through the F-junction (which is typically rate-limiting), but simultaneously generates FADH2 and NADH and thus depends on [[N-junction]] throughput. Hence FAO can be inhibited completely by inhibition of [[Complex I]] (CI). This physiological substrate combination is required for partial reconstitution of [[TCA cycle]] function and convergent electron-input into the [[Q-junction]], to compensate for metabolite depletion into the incubation medium. FNS in combination exerts an [[additive effect of convergent electron flow]] in most types of mitochondria.[[additive effect of convergent electron flow]] in most types of mitochondria.)
Q-junction + ([[File:SUIT-catg FNSGp.jpg|right|300px|Q-j … [[File:SUIT-catg FNSGp.jpg|right|300px|Q-junction]]The '''Q-junction''' is a junction for [[convergent electron flow]] in the [[Electron transfer pathway]] (ET-pathway) from type N substrates and mt-matrix dehydrogenases through [[Complex I]] (CI), from type F substrates and FA oxidation through [[electron-transferring flavoprotein Complex]] (CETF), from succinate (S) through [[Complex II]] (CII), from glycerophosphate (Gp) through [[glycerophosphate dehydrogenase Complex]] (CGpDH), from choline through [[choline dehydrogenase]], from dihydro-orotate through [[dihydro-orotate dehydrogenase]], and other enzyme Complexes into the Q-cycle (ubiquinol/ubiquinone), and further downstream to [[Complex III]] (CIII) and [[Complex IV]] (CIV). The concept of the Q-junction, with the [[N-junction]] and [[F-junction]] upstream, provides the rationale for defining [[Electron-transfer-pathway state]]s and [[categories of SUIT protocols]].[[categories of SUIT protocols]].)
Electron-transfer-pathway state + ([[File:SUIT-catg FNSGpCIV.jpg|right|400px] … [[File:SUIT-catg FNSGpCIV.jpg|right|400px]]'''Electron-transfer-pathway states''' are obtained in [[mitochondrial preparations]] (isolated mitochondria, permeabilized cells, permeabilized tissues, tissue homogenate) by depletion of endogenous substrates and addition to the mitochondrial respiration medium of fuel substrates (CHNO) activating specific mitochondrial pathways, and possibly inhibitors of specific pathways. Mitochondrial electron-transfer-pathway states have to be defined complementary to mitochondrial [[coupling-control state]]s. [[Coupling-control state]]s require [[Electron-transfer-pathway state|ET-pathway competent states]], including oxygen supply. [[Categories of SUIT protocols]] are defined according to mitochondrial ET-pathway states.» [[#ET_pathway_states |'''MiPNet article''']][#ET_pathway_states |'''MiPNet article''']])
N-junction + ([[File:SUIT-catg N.jpg|right|300px|N-junct … [[File:SUIT-catg N.jpg|right|300px|N-junction]]The '''N-junction''' is a junction for [[convergent electron flow]] in the [[electron transfer pathway]] (ET-pathway) from type N substrates (''further details'' »[[N-pathway control state]]) through the mt-[[NADH]] pool to [[Complex I]] (CI), and further transfer through the [[Q-junction]] to [[Complex III]] (CIII). Representative type N substrates are pyruvate (P), glutamate (G) and malate (M). The corresponding dehydrogenases ([[Pyruvate dehydrogenase |PDH]], [[Glutamate dehydrogenase |GDH]], [[Malate dehydrogenase |MDH]]) and some additional TCA cycle dehydrogenases ([[isocitrate dehydrogenase]], [[oxoglutarate dehydrogenase]] generate NADH, the substrate of [[Complex I]] (CI). The concept of the N-junction and [[F-junction]] provides a basis for defining [[categories of SUIT protocols]] based on [[Electron-transfer-pathway state]]s.[Electron-transfer-pathway state]]s.)
NADH electron transfer-pathway state + ([[File:SUIT-catg N.jpg|right|300px|N-junct … [[File:SUIT-catg N.jpg|right|300px|N-junction]]The '''NADH electron transfer-pathway state''' (N) is obtained by addition of [[NADH]]-linked substrates (CI-linked), feeding electrons into the [[N-junction]] catalyzed by various mt-dehydrogenases. N-supported flux is induced in mt-preparations by the addition of NADH-generating substrate combinations of [[pyruvate]] (P), [[glutamate]] (G), [[malate]] (M), [[oxaloacetate]] (Oa), [[oxoglutarate]] (Og), [[citrate]], [[hydroxybutyrate]]. These N-junction substrates are (indirectly) linked to [[Complex I]] by the corresponding dehydrogenase-catalyzed reactions reducing NAD+ to NADH+H+ + H+. The most commonly applied N-junction substrate combinations are: [[PM]], [[GM]], [[PGM]]. The [[malate-anaplerotic pathway control state]] (M alone) is a special case related to [[malic enzyme]] (mtME). The [[glutamate-anaplerotic pathway control state]] (G alone) supports respiration through [[glutamate dehydrogenase]] (mtGDH). Oxidation of [[tetrahydrofolate]] is a NAD(P)H linked pathway with formation of formate. In mt-preparations, succinate dehydrogenase (SDH; [[CII]]) is largely substrate-limited in N-linked respiration, due to metabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the N-pathway control state can be further suppressed by the CII-inhibitor [[malonic acid]]). In the N-pathway control state [[Electron-transfer-pathway state|ET pathway level 4]] is active.[[Electron-transfer-pathway state|ET pathway level 4]] is active.)
NS-pathway control state + ([[File:SUIT-catg NS.jpg|right|300px|NS-pat … [[File:SUIT-catg NS.jpg|right|300px|NS-pathway control]]'''NS-pathway control''' is exerted in the NS-linked substrate state (flux in the NS-linked substrate state, NS; or Complex I&II, CI&II-linked substrate state). NS-OXPHOS capacity provides an estimate of physiologically relevant maximum mitochondrial respiratory capacity. NS is induced in mt-preparations by addition of [[NADH]]-generating substrates ([[N-pathway control state]] in combination with [[succinate]] ([[Succinate pathway]]; S). Whereas NS expresses substrate control in terms of substrate types (N and S), CI&II defines the same concept in terms of convergent electron transfer to the [[Q-junction]] (pathway control). '''NS''' is the abbreviation for the combination of [[NADH]]-linked substrates (N) and [[succinate]] (S). This physiological substrate combination is required for partial reconstitution of [[TCA cycle]] function and convergent electron-input into the [[Q-junction]], to compensate for metabolite depletion into the incubation medium. NS in combination exerts an [[additive effect of convergent electron flow]] in most types of mitochondria.[[additive effect of convergent electron flow]] in most types of mitochondria.)
Glycerophosphate pathway control state + ([[File:SUIT-catg_Gp.jpg|right|300px|Gp-pat … [[File:SUIT-catg_Gp.jpg|right|300px|Gp-pathway]]The '''glycerophosphate pathway control state''' (Gp) is an [[Electron-transfer-pathway state |ET-pathway level 3 control state]], supported by the fuel substrate [[glycerophosphate]] and electron transfer through [[glycerophosphate dehydrogenase Complex]] into the [[Q-junction]]. The [[glycerolphosphate shuttle]] represents an important pathway, particularly in liver and blood cells, of making cytoplasmic [[NADH]] available for mitochondrial [[oxidative phosphorylation]]. Cytoplasmic NADH reacts with dihydroxyacetone phosphate catalyzed by cytoplasmic glycerophos-phate dehydrogenase. On the outer face of the inner mitochondrial membrane, mitochondrial glycerophosphate dehydrogenase oxidises glycerophosphate back to dihydroxyacetone phosphate, a reaction not generating NADH but reducing a flavin prosthesic group. The reduced flavoprotein donates its reducing equivalents to the electron transfer-pathway at the level of [[CoQ]].[[CoQ]].)
Categories of SUIT protocols + ([[File:SUIT-catg_MitoPathway types.jpg|rig … [[File:SUIT-catg_MitoPathway types.jpg|right|200px]]'''Categories of SUIT protocols''' group [[MitoPedia: SUIT |SUIT protocols]] according to all substrate types involved in a protocol (F, N, S, Gp), independent of the sequence of titrations of substrates and inhibitors which define the [[Electron-transfer-pathway state]]s. The [[N-pathway control |N-type substrates]] are listed in parentheses, independent of the sequence of titrations. ROX states may or may not be included in a SUIT protocol, which does not change its category. Similarly, the [[CIV]] assay may or may not be added at the end of a SUIT protocol, without effect on the category of a SUIT protocol.* '''F''' - ET-pathway-level 5: [[FADH2 |FADH2]]-linked substrates (FAO) with obligatory support by the N-linked pathway.* '''N''' - ET-pathway-level 4: [[NADH]]-linked substrates (CI-linked).* '''S''' - ET-pathway-level 3: [[Succinate]] (CII-linked).* '''Gp''' - ET-pathway-level 3: [[Glycerophosphate]] (CGpDH-linked).* '''Y(X)'''- In the SUIT general protocols Y makes reference to the ET-pathway state and X to the combination os substrates added for the corresponding pathway.» [[#Categorization of SUIT protocols: ETS pathway control states |'''MiPNet article''']][#Categorization of SUIT protocols: ETS pathway control states |'''MiPNet article''']])
Succinate pathway + ([[File:SUIT-catg_S.jpg|right|300px|Succina … [[File:SUIT-catg_S.jpg|right|300px|Succinate]]The '''Succinate pathway''' (S-pathway; S) is the [[electron transfer pathway]] that supports succinate-linked respiration (succinate-induced respiratory state; previously used nomenclature: CII-linked respiration; SRot; see [[Gnaiger 2009 Int J Biochem Cell Biol]]). The S-pathway describes the electron flux through [[Complex II]] (CII; see [[succinate dehydrogenase]], SDH) from succinate and FAD to fumarate and CII-bound flavin adenine dinucleotide (FADH2) to the [[Q-junction]].The S-pathway control state is usually induced in mt-preparations by addition of succinate&rotenone. In this case, only [[Complex III]] and [[Complex IV]] are involved in pumping protons from the matrix (positive phase, P-phase) to the negative phase (N-phase) with a P»/O2 of 3.5 (P»/O ratio = 1.75).phase) with a P»/O2 of 3.5 (P»/O ratio = 1.75).)
SUIT protocol names + ([[File:SUIT-nomenclature.jpg|300px|right|S … [[File:SUIT-nomenclature.jpg|300px|right|SUIT protocols]]The '''SUIT protocol name''' starts with (i) the [[Categories of SUIT protocols |SUIT category]] which shows the [[Electron-transfer-pathway state]]s (ET pathway types; e.g. N, S, NS, FNS, FNSGp), independent of the actual sequence of titrations. (ii) A further distinction is provided in the SUIT name by listing in parentheses the substrates applied in the [[N-pathway control state]]s, again independent of the sequence of titrations, e.g. NS(GM), NS(PM), FNSGp(PGM). (iii) A sequentially selected number is added, e.g. SUIT_FNS(PM)01 (see [[Coupling/pathway control diagram]]). The '''systematic name''' of a SUIT protocol starts with the [[Categories of SUIT protocols |SUIT category]], followed by an underline dash and the sequence of titration steps (mark names, #''X'', separated by a comma). The [[Marks in DatLab |Marks]] define the section of a [[respiratory state]] in the SUIT protocol. The [[Mark names in DatLab |Mark name]] contains the sequential number and the [[metabolic control variable]], ''X''. The metabolic control variable is the name of the preceding SUIT [[event]]. The [[MitoPedia: SUIT |MitoPedia list of SUIT protocols]] can be sorted by the short name or the systematic name (hence by SUIT protocol category. The '''[[SUIT protocol pattern]]''' is best illustrated by a [[coupling/pathway control diagram]].[[coupling/pathway control diagram]].)
Coupling/pathway control diagram + ([[File:SUIT-nomenclature.jpg|300px|right|S … [[File:SUIT-nomenclature.jpg|300px|right|SUIT protocols]]'''Coupling/pathway control diagrams''' illustrate the respiratory '''states''' obtained step-by-step in substrate-uncoupler-inhibitor titrations in a [[SUIT protocol]]. Each step (to the next state) is defined by an initial state and a [[metabolic control variable]], ''X''. The respiratory states are shown by boxes. ''X'' is usually the titrated substance in a SUIT protocol. If ''X'' ([[ADP]], [[uncoupler]]s, or inhibitors of the [[phosphorylation system]], e.g. oligomycin) exerts '''coupling control''', then a transition is induced between two [[coupling-control state]]s. If ''X'' (fuel substrates, e.g. pyruvate and succinate, or [[Electron transfer pathway]] inhibitors, e.g. rotenone) exerts '''pathway control''', then a transition is induced between two [[Electron-transfer-pathway state]]s. The type of metabolic control (''X'') is shown by arrows linking two respiratory states, with vertical arrows indicating coupling control, and horizontal arrows indicating pathway control. [[Marks - DatLab |Marks]] define the section of an experimental trace in a given [[respiratory state]] (steady state). [[Events - DatLab |Events]] define the titration of ''X'' inducing a transition in the SUIT protocol. The specific sequence of coupling control and pathway control steps defines the [[SUIT protocol pattern]]. The coupling/pathway control diagrams define the [[categories of SUIT protocols]] (see Figure).[[categories of SUIT protocols]] (see Figure).)
SUIT-013 + ([[File:SUIT013 AmR ce D023.png|300px]])
SUIT-013 AmR ce D023 + ([[File:SUIT013 AmR ce D023.png|400px]])
Sample Holder + ([[File:Sample Holder - 28410-01.jpg|right|180px]] Sample Holder - to protect susceptible samples from being damaged by stirring of the medium in the 2.0 mL O2k-chamber.)
O2k series + ([[File:Seires number H-Seires.png|right|20 … [[File:Seires number H-Seires.png|right|200 px|The serial number of each O2k is shown on a sticker at the rear of the O2k.]]The '''O2k series''' is specified as the capital letter in the O2k serial number of the [[Oroboros O2k]]. A serial number G-#### or H-#### denotes an Oxygraph from the G or H series, while A-#### denotes an O2k from the A series. With [[DatLab]] running real-time connected to the O2k, the serial number of the currently connected O2k is displayed: (1) in the right corner of the [[O2k status line|status line]], besides the DatLab version number (bottom), and (2) in windows [[O2k control]] [F7] and [[O2k configuration]].[[O2k configuration]].)
Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port + ([[File:Stopper black PEEK angular Shaft si … [[File:Stopper black PEEK angular Shaft side+6.2+2.6 mm Port.JPG|180px|right]]'''Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port''', for application with [[ISE]]; side titration port and two additional holes (6.2 mm and 2.6 mm); angular bottom; including [[Volume-Calibration Ring]] (A or B); 2 mounted O-rings, with 8 spare O-rings ([[O-ring\Viton\12.5x1 mm]]).[[O-ring\Viton\12.5x1 mm]]).)
Stopper\black PEEK\conical Shaft\central+2.3+2.6 mm Port + ([[File:Stopper black PEEK conical Shaft ce … [[File:Stopper black PEEK conical Shaft central+2.3+2.6 mm Port.JPG|180px|right]]'''Stopper\black PEEK\conical Shaft\central+2.3+2.6 mm Port''': for pH and reference electrode, central titration port and two additional ports (2.3 mm and 2.6 mm); conical bottom; including Volume-Calibration Ring (A or B), 2 mounted O-rings, with 8 spare O-rings ([[O-ring\Viton\12.5x1 mm]]).[[O-ring\Viton\12.5x1 mm]]).)
Succinate + ([[File:Succinic_acid.jpg|left|100px|Succin … [[File:Succinic_acid.jpg|left|100px|Succinic acid]]'''Succinic acid''', C4H6O4, (butanedioic acid) is a dicarboxylic acid which occurs under physiological conditions as the anion '''succinate2-, S''', with ''p''Ka1 = 4.2 and ''p''Ka2 = 5.6. Succinate is formed in the [[TCA cycle]], and is a substrate of [[Complex II |CII]], reacting to [[fumarate]] and feeding electrons into the [[Q-junction]]. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction. Succinate is transported across the inner mt-membrane by the [[dicarboxylate carrier]]. The plasma membrane of many cell types is impermeable for succinate (but see [[Zhunussova 2015 Am J Cancer Res]] for an exception). Incubation of mt-preparations by succinate alone may lead to accumulation of [[oxaloacetate]], which is a potent inhibitor of Complex II (compare [[Succinate and rotenone]]). High activities of mt-[[Malic enzyme]] (mtME) prevent accumulation of oxaloacetate in incubations with succinate without rotenone.[[Malic enzyme]] (mtME) prevent accumulation of oxaloacetate in incubations with succinate without rotenone.)
Syringe\500 mm3 51/0.41 mm + ([[File:Syringe 500 mm3 51 0.41 mm.JPG |rig … [[File:Syringe 500 mm3 51 0.41 mm.JPG |right|180px]]Hamilton '''Syringe\500 mm3 51/0.41 mm''' for manual titrations, 500 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.41 mm inner diameter; for injections of suspensions of isolated mitochondria and filling of the [[Microsyringe\200 mm3\TIP2k]].[[Microsyringe\200 mm3\TIP2k]].)
Syringe Labels + ([[File:Syringe Labels_2017.JPG|right|180px]]'''Syringe Labels''': set of labels with standard abbreviations (see [[MiPNet09.12_O2k-Titrations | O2k-Titrations]]).)
Syringe Racks + ([[File:Syringe Racks.JPG|right|180px]]'''Syringe Racks''': stainless steel; for proper placement of eight Hamilton microsyringes in HRR experiments; package of two (for a total of 16 microsyringes).)
Syringe Storage Box + ([[File:Syringe Storage Box.jpg|right|180px]]'''Syringe Storage Box''': for storing the Hamilton microsyringes; includes [[Syringe Labels]].)
TIP2k-Module + ([[File:TIP2k with 200 mm3 microsyringe.JPG … [[File:TIP2k with 200 mm3 microsyringe.JPG|180px|right]]'''TIP2k-Module''' - Titration-Injection microPump (TIP2k) for two-channel operation with the [[O2k-FluoRespirometer]] with automatic control by [[DatLab]] of programmable titration regimes and feedback control (oxystat, pH-stat).s and feedback control (oxystat, pH-stat).)
O2k-TPP+ ISE-Module + ([[File:TPP new.jpg|180px|right]]'''O2k-TPP+ ISE-Module''': Potentiometric ion-selective electrodes for measurement of mitochondrial membrane potential)
Avogadro constant + ([[File:Table Physical constants.png|left|4 … [[File:Table Physical constants.png|left|400px|thumb|]] {''Quote''} The '''Avogadro constant''' ''N''A is a proportionality constant between the quantity [[amount]] of substance (with unit [[mole]]) and the quantity for [[count |counting entities]] ... One mole contains exactly 6.022 140 76 × 1023 elementary [[entity |entities]]. This number is the fixed numerical value of the Avogadro constant, ''N''A, when expressed in the unit mol−1 and is called the Avogadro number {''End of Quote'': [[Bureau International des Poids et Mesures 2019 The International System of Units (SI)]]}. Thus the Avogadro constant ''N''A has the SI unit 'per mole' [mol-1], but more strictly the unit for counting entities per amount is 'units per mole' [x·mol-1] (compare [[elementary charge]]). Therefore, ''N''A is 'count per amount' with units 'counting units per mole'. The Avogadro constant times elementary charge is the [[Faraday constant]].raday constant]].)
Boltzmann constant + ([[File:Table Physical constants.png|left|4 … [[File:Table Physical constants.png|left|400px|thumb|]] The '''Boltzmann constant''' ''k'' has the SI unit [J·K-1] (IUPAC), but more strictly the units for energy per particles per temperature is [J·x-1·K-1]. ''k'' = ''f''·''e''-1, the [[electrochemical constant]] ''f'' times the [[elementary charge]] ''e''. ''k'' = ''R''·''N''A-1, the [[gas constant]] ''R'' divided by the [[Avogadro constant]] ''N''A.gadro constant]] ''N''A.)
Gas constant + ([[File:Table Physical constants.png|left|4 … [[File:Table Physical constants.png|left|400px|thumb|]] The '''gas constant''', ''R'' = 8.314462618 J·mol-1·K-1, has the SI unit for energy per amount per temperature. ''R'' is primarily known from the ideal gas equation, ''pV'' = ''nRT'' or ''p'' = ''cRT''. Therefore, ''RT'' is the ratio of pressure ''p'' and concentration ''c''. ''R'' = ''f''·''F'', the [[electrochemical constant]] ''f'' times the [[Faraday constant]] ''F''. ''R'' = ''k''·''N''A, the [[Boltzmann constant]] ''k'' times the [[Avogadro constant]] ''N''A.ogadro constant]] ''N''A.)
Electrochemical constant + ([[File:Table Physical constants.png|right| … [[File:Table Physical constants.png|right|400px|thumb|]] The '''electrochemical constant''' ''f'' has the SI unit for energy per charge per temperature [J·C-1·K-1]. ''f'' = ''k''·''e''-1, the [[Boltzmann constant]] ''k'' divided by the [[elementary charge]] ''e''. ''f'' = ''R''·''F''-1, the [[gas constant]] ''R'' divided by the [[Faraday constant]] ''F''.raday constant]] ''F''.)
Format + ([[File:Table Physical constants.png|right| … [[File:Table Physical constants.png|right|600px|thumb|Converstion between different motive formats and corresponding motive units ([[Gnaiger 2020 BEC MitoPathways]])]].Different '''formats''' can be chosen to express physicochemical quantities ([[motive entity |motive entities]] or transformants) in corresponding [[motive unit]]s [MU]. Fundamental formats for electrochemical transformations are:* ''N'': particle or molecular format of a count; MU = x * ''n'': chemical or molar format of amount; MU = mol * ''e'': electrical format of charge; MU = C* ''m'': mass format; MU = kg* ''V'': volume format; MU = m3* ''G'': exergy format; MU = J* ''H'': enthalpy format; MU = J* ''S'': entropy format; MU = J·K-1lt;/u>: exergy format; MU = J * ''H'': enthalpy format; MU = J * ''S'': entropy format; MU = J·K-1)
Elementary charge + ([[File:Table Physical constants.png|right| … [[File:Table Physical constants.png|right|400px|thumb|]] The '''elementary charge''' or proton charge ''e'' has the SI unit coulomb [C], but more strictly coulomb per elementary unit [C·x-1]. -''e'' is the charge per electron. Elementary charge ''e'' is the charge per [[elementary entity]] H+ with SI unit [C] but canonical SI unit [C·x-1]. When the charge ''Q''el [C] of a number ''N''e [x] of electrons e is divided by the count ''N''e, then the [[particle charge]] ''QNX'' (''QNX'') charge per elementary entity is obtained, -''e'' = ''Q''el/''N''e [C·x-1]. ''e'' is also used as an atomic unit.X'') charge per elementary entity is obtained, -''e'' = ''Q''el/''N''e [C·x-1]. ''e'' is also used as an atomic unit.)
T-Shirt: MitoFit + ([[File:Tshirt MitoFit OROBOROS.jpg|right|180px]] '''T-Shirt MitoFit''': Oroboros Logo on front, MitoFit Logo on back.)
Tube Racks + ([[File:Tube Racks.JPG|right|180px]]'''Tube Racks''': stainless steel, accomodating four 50-ml tubes, for cleaning of microsyringes, cleaning and storage of stoppers during cleaning of the [[O2k-chamber]]s; package of two (for a total of eight tubes).)
United Mitochondrial Disease Foundation + ([[File:UMDF LOGO.JPG|200px|left|UMDF]] The … [[File:UMDF LOGO.JPG|200px|left|UMDF]]The '''United Mitochondrial Disease Foundation''' (UMDF) was founded in 1996 to promote research and education for the diagnosis, treatment and cure of mitochondrial disorders and to provide support to affected individuals and families.port to affected individuals and families.)
V-ring\30-35-4.5 mm + ([[File:V-Ring 30-35-4.5.JPG|right|180px]] '''V-ring\30-35-4.5 mm''', mounted on [[O2k-Chamber Holder]] and [[O2k-Chamber Holder sV]].)
O-ring\Viton\18x2 mm + ([[File:Viton O-ring 18x2.jpg|right|180px|link=]] '''O-ring\[[Viton]]\18x2 mm''', mounted on the [[O2k-Chamber Holder]].)
SUIT-003 O2 ce D039 + ([[File:ce1;(ce2Omy);ce3U;ce4Rot;ce5Ama.jpg|300px]])
SUIT-003 O2 ce D061 + ([[File:ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO;ce6DMSO;ce7Ama.png|400px]])
SUIT-003 O2 ce D060 + ([[File:ce1;(ce2Omy);ce3U;ce4Rot;ce5Snv;ce6Mnanv;ce7Ama.png|400px]])
SUIT-002 O2 ce-pce D007a + ([[File:ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px|SUIT-RP2 for PBMC and PLT]])
SUIT-002 O2 ce-pce D007 + ([[File:ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px|SUIT-RP2]])
SUIT-008 O2 ce-pce D025 + ([[File:ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Az.png|600px]])
SUIT-012 O2 ce-pce D052 + ([[File:ce1;1Dig;1PM;2D;2c;3G;4U;5Ama.png|450px]])
SUIT-001 O2 ce-pce D004 + ([[File:ce1;1Dig;1PM;2D;2c;3U;4G;5S;(6Oct);7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1 for PBMC and PLT]])
SUIT-001 O2 ce-pce D003 + ([[File:ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|600px|SUIT-RP1]])
SUIT-006 MgG ce-pce D085 + ([[File:ce1;1Dig;1PM;2D;3Cat;4U;5Ama.png|400px]])
SUIT-006 Q ce-pce D073 + ([[File:ce1;1Dig;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.png|400px]])
SUIT-026 O2 ce-pce D088 + ([[File:ce1;1Dig;1S;2Rot;3D;3c;4Ama.png|400px]])
SUIT-003 O2 ce D062 + ([[File:ce1;ce1DMSO;(ce2Omy);ce3U;ce4Rot;ce5Ama.png|400px]])
SUIT-003 AmR ce D059 + ([[File:ce1;ce1H2O;ce1MiR05;ce1DMSO;ce2Omy;ce3U;ce4Rot;ce5Ama.png|500px]])
SUIT-003 O2 ce-pce D013 + ([[File:ce1;ce1P;ce2Omy;ce3U;ce4Glc;ce5M;ce6Rot;ce7S;1Dig;1c;2Ama;3AsTm;4Azd.png|600px]])
SUIT-003 O2 ce-pce D020 + ([[File:ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd.png|600px]])
SUIT-003 Ce1;ce1P;ce3U;ce4Glc;ce5M;ce6Rot;ce7S;1Dig;1c;2Ama;3AsTm;4Azd + ([[File:ce1;ce1P;ce3U;ce4Glc;ce5M;ce6Rot;ce7S;1Dig;1c;2Ama;3AsTm;4Azd.png|600px]])
SUIT-003 Ce1;ce1SD;ce2Omy;ce3U- + ([[File:ce1;ce1SD;ce2Omy;ce3U;ce4Rot;ce5Ama.png|200px]])
SUIT-003 AmR ce D058 + ([[File:ce1;ce1SOD;ce1HRP;ce1AmR;ce2Omy;ce3U;ce4Rot;ce5Ama.png|500px]])
SUIT-003 O2 ce D050 + ([[File:ce1;ce1Snv;(ce2Omy);ce3U;ce4Rot;ce5Ama.png|400px]])
SUIT-022 O2 ce D051 + ([[File:ce1;ce2KCN;ce3SHAM.v2.png|350px]] [[File:ce1;ce2KCN;ce3SHAM.png|350px]])
SUIT-022 + ([[File:ce1;ce2KCN;ce3SHAM.v2.png|400px]])
SUIT-003 O2 ce D028 + ([[File:ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama.png|500px]])
SUIT-003 AmR ce D017 + ([[File:ce1;ce2P;ce3Omy;ce4U;ce5Rot;ce6S;ce7Ama.png|500px]])
SUIT-010 + ([[File:ce1;ce2Rot;ce3S;ce4D;1Dig;1c.png|400px|Respirometric test of optimum digitonin concentration ]])
SUIT-010 O2 ce-pce D008 + ([[File:ce1;ce2Rot;ce3S;ce4D;1Dig;1c.png|400px|Respirometric test of optimum digitonin concentration ]])
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U- + ([[File:ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama.png|200px]])
SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama + ([[File:ce1;ce2SD;ce3U;ce4Rot;ce5Ama.png|200px]])
SUIT-023 O2 ce D053 + ([[File:ce1;ce2SHAM;ce3KCN.png|350px]])
SUIT-023 + ([[File:ce1;ce2SHAM;ce3KCN.png|400px]])
SUIT-006 02 mt D108 + ([[File:mt;1PGM;2D;2c;(3Omy);4U;5Myx.png|300 px]])
SUIT-006 NADH mt D084 + ([[File:mt;1PGM;2D;3(Omy);4U;5Anox;6Myx;7Reox.png|350px]])
SUIT-006 Q mt D071 + ([[File:mt;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.png|400px]])
Stopper\white PVDF\conical Shaft\central Port + ([[Image:30221-24 PVDF Stopper.jpg|right|18 … [[Image:30221-24 PVDF Stopper.jpg|right|180px]] '''Stopper\white PVDF\conical Shaft\central Port''': for closing the 2-mL [[O2k-chamber]], with one capillary and conical basis; including [[Volume-Calibration Ring]] (A or B) for volume adjustment (1.5 to 3.2 mL); 2 mounted O-rings ([[O-ring\Viton\12x1 mm]]).'''Discontinued '''[[O-ring\Viton\12x1 mm]]). '''Discontinued ''')
TIP2k-Needle Spacer + ([[Image:80600-24 Spacers f. TIP2k needle.JPG|right|180px]]'''TIP2k-Needle Spacers''' for microinjection TIP2k needle, silicone stops (200/Pkg.) with mounting tool.)
ISE-Ca2+ Membranes + ([[Image:Ca2+ membranes.jpg|right|180px]]'''ISE-Ca2+ Membranes''': PVC, 4 mm diameter, box of 5 membranes. To be used with the [[O2k-TPP+ ISE-Module]].)