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- Oxygen solubility factor + (The '''oxygen solubility factor''' of the … The '''oxygen solubility factor''' of the incubation medium, ''F''<sub>M</sub>, expresses the effect of the salt concentration on [[oxygen solubility]] relative to pure water. In mitochondrial respiration medium [[MiR05]], [[MiR05-Kit]] and [[MiR06]], ''F''<sub>M</sub> is 0.92 (determined at 30 and 37 °C) and in culture media is 0.89 (at 37 °C). ''F''<sub>M</sub> varies depending on the temperature and composition of the medium. To determine the FM based on the oxygen concentration, specific methods and equipment are needed (see references Rasmussen HN, Rasmussen UF 2003 in [https://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP MiPNet06.03]). For other media, ''F''<sub>M</sub> may be estimated using Table 4 in [https://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP MiPNet06.03]. For this purpose KCl based media can be described as "seawater" of varying salinity. The original data on sucrose and KCl-media (Reynafarje et al 1985), however, have been critizesed as artefacts and the ''F''<sub>M</sub> of 0.92 is suggested in the temperature range of 10 °C to 40 °C as for MiR05.<sub>M</sub> of 0.92 is suggested in the temperature range of 10 °C to 40 °C as for MiR05.)
