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- Permeabilized muscle fibers + ('''Permeabilized muscle fibers''' (pfi) ar … '''Permeabilized muscle fibers''' (pfi) are used as a mitochondrial preparation in respirometry to access mitochondrial function comparable to [[isolated mitochondria]] (imt). pfi are obtained by selectively permeabilizing the plasma membrane mechanically and chemically ([[saponin]]), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes.</br></br>:» MitoPedia topic: [[Mitochondrial preparations]][Mitochondrial preparations]])
