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- Magnesium Green + ('''Magnesium Green''' (MgG) is an [[extrinsic fluorophores|extrinsic fluorophore]] … '''Magnesium Green''' (MgG) is an [[extrinsic fluorophores|extrinsic fluorophore]] that fluoresces when bound to Mg<sup>2+</sup> and is used for measuring mitochondrial ATP production by [[mitochondrial preparations]]. Determination of mitochondrial ATP production is based on the different dissociation constants of Mg<sup>2+</sup> for [[ADP]] and [[ATP]], and the exchange of one ATP for one ADP across the mitochondrial inner membrane by the [[adenine nucleotide translocase]] (ANT). Using the dissociation constants for ADP-Mg<sup>2+</sup> and ATP-Mg<sup>2+</sup> and initial concentrations of ADP, ATP and Mg<sup>2+</sup>, the change in ATP concentration in the medium is calculated, which reflects mitochondrial ATP production. change in ATP concentration in the medium is calculated, which reflects mitochondrial ATP production.)