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- Oxygen flux - instrumental background + ('''Instrumental background oxygen flux''', ā¦ '''Instrumental background oxygen flux''', ''J''Ā°<sub>O<sub>2</sub></sub>, in a respirometer is due to oxygen consumption by the [[POS]], and oxygen diffusion into or out of the aqueous medium in the [[O2k-chamber]]. It is a property of the instrumental system, measured in the range of experimental oxygen levels by a standardized instrumental O<sub>2</sub> background test. The oxygen regime from air saturation towards zero oxygen is applied generally in experiments with isolated mitochondria, and living or permeabilized cells. To overcome oxygen diffusion limitation in permeabilized fibers and homogenates, an elevated oxygen regime is applied, requiring instrumental background test in the same range of elevated oxygen., requiring instrumental background test in the same range of elevated oxygen.)
