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- 2-mercaptoacetate + ('''2-mercaptoacetate''' is an inhibitor of … '''2-mercaptoacetate''' is an inhibitor of medium-chain acyl-CoA dehydrogenase, MCAD, the rate-limiting enzyme of [[octanoylcarnitine]] oxidation. 2-mercaptoacetate has been used as an inhibitor of [[fatty acid oxidation]] ([[F-pathway control state]]). In permeabilized rat soleus muscle fibers, pre-incubation with 1 mM 2-mercaptoacetate for 45 min resulted in 58% inhibition of MCAD and decreased [[octanoylcarnitine]]&[[malate]] stimulated respiration by approximately 60% ([[Osiki 2016 FASEB J]]).[[Osiki 2016 FASEB J]]).)