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Difference between revisions of "Solutions"

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== Applications on the Bioblast wiki ==
== Applications on the Bioblast wiki ==
: In various protocols described on the Bioblast wiki, clarification and updates are required to provide consistent information on storage versus stock concentrations.
: In various protocols described on the Bioblast wiki, clarification and updates are required to provide consistent information on storage versus stock concentrations. The use of 'working concentrations' is discouraged.

Revision as of 13:55, 19 August 2013


high-resolution terminology - matching measurements at high-resolution


Solutions

Description

A solution is {quote}: A liquid or solid phase containing more than one substance, when for convenience one (or more) substance, which is called the solvent, is treated differently from the other substances, which are called solutes. When, as is often but not necessarily the case, the sum of the mole fractions of solutes is small compared with unity, the solution is called a dilute solution. A superscript attached to the ∞ symbol for a property of a solution denotes the property in the limit of infinite dilution {end of quote: IUPAC Gold Book}.

Abbreviation: n.a.

Reference: IUPAC Gold Book



MitoPedia topics: Media for respirometry 

Working with solutions at different dilutions

  • Final concentration: The concentration in solution used in the experimental chamber, at a dilution that depends on the stock concentration, the volume added (v) and the volume of the experimental chamber (V). The final concentration may change in the course of an experiment, if the substance is involved in chemical reactions and subsequent titrations induce a further dilution or provide further additions in the experimental system.
  • Stock solution, stock concentration: The stock solution used as a stock during the experiment, for filling titration syringes and additions to the experimental chamber; the stock solution differs from the storage solution, if it has to be prepared fresh and cannot be stored.
  • Storage solution, storage concentration: The solution used for storage. There may be different storage concentrations, if various dilutions can be stored. In specific cases, the storage solution may be equal to the stock solution, if the stock solution can be stored.
  • Commercial stock; commercial solution: The commercial stock is frequently a dry powder, or a solution. It is appropriate for storage according to the suppliers specifications. Once opened, storage conditions and maximum storage times may change. For some chemicals the entire commercial stock may have to be used at once for preparation of a storage solution.
  • Working concentration: This is an ambiguous term. Clarification is required if it is used as the final concentration or stock concentration defined above. Perhaps working solutions should be considered to comprise both the final and stock solutions, in contrast to storage and commercial solutions.

Applications on the Bioblast wiki

In various protocols described on the Bioblast wiki, clarification and updates are required to provide consistent information on storage versus stock concentrations. The use of 'working concentrations' is discouraged.