Sodium phosphate buffer: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Na-PB | |abbr=Na-PB | ||
|description= Sodium phosphate buffer, '''Na-PB''', for [[HRR]] with freeze-dried baker´s yeast | |description=Sodium phosphate buffer, '''Na-PB''', for [[HRR]] with freeze-dried baker´s yeast. | ||
}} | }} | ||
{{MitoPedia | {{MitoPedia O2k and high-resolution respirometry|type=Chemicals | ||
|type=Chemicals | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Medium | |mitopedia topic=Medium | ||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
* '''Oxygen solubility factor''' of Na-PB = '''0.95''' | * '''Oxygen solubility factor''' of Na-PB = '''0.95''' | ||
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== Na-PB (50 mM, pH 7.1)== | == Na-PB (50 mM, pH 7.1)== | ||
Dissolve 3.9 g NaH<sub>2</sub>PO<sub>4</sub> x 2 H<sub>2</sub>O (Sigma 71505, | Dissolve 3.9 g NaH<sub>2</sub>PO<sub>4</sub> x 2 H<sub>2</sub>O (Sigma 71505, M = 156.01 g·mol<sup>-1</sup>) in 500 mL a.dest and adjust pH with 10 M NaOH (Sigma 72068, M = 40.00 g·mol<sup>-1</sup>) to 7.1. | ||
Latest revision as of 13:10, 12 February 2021
- high-resolution terminology - matching measurements at high-resolution
Sodium phosphate buffer
Description
Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast.
Abbreviation: Na-PB
MitoPedia topics: Media for respirometry
Application in HRR
- Oxygen solubility factor of Na-PB = 0.95
Na-PB (50 mM, pH 7.1)
Dissolve 3.9 g NaH2PO4 x 2 H2O (Sigma 71505, M = 156.01 g·mol-1) in 500 mL a.dest and adjust pH with 10 M NaOH (Sigma 72068, M = 40.00 g·mol-1) to 7.1.
