Difference between revisions of "Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
From Bioblast
Line 10: | Line 10: | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|preparations=Permeabilized tissue | |||
|couplingstates=OXPHOS, ETS | |couplingstates=OXPHOS, ETS | ||
|substratestates=FAO | |substratestates=FAO | ||
|additional=Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine | |additional=Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine | ||
}} | }} |
Revision as of 17:23, 11 November 2015
Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Smith CD (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine.
Protocol: For maximal respiration we make sequential additions of 18 Β΅M PC, 20 Β΅M PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition.
Labels:
Preparation: Permeabilized tissue
Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine