Sjoevall 2013 Mitochondrion: Difference between revisions
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{{Publication | {{Publication | ||
|title=SjΓΆvall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, ArnbjΓΆrnsson E, Hansson | |title=SjΓΆvall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, ArnbjΓΆrnsson E, Hansson Magnus J, Fellman V, ElmΓ©r E (2013) Mitochondrial respiration in human viable platelets - methodology and influence of gender, age and storage. Mitochondrion 13:7-14. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/23164798 PMID: 23164798 Open Access] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/23164798 PMID: 23164798 Open Access] | ||
|authors=Sjoevall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjoernsson E, Hansson | |authors=Sjoevall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjoernsson E, Hansson Magnus J, Fellman V, Elmer E | ||
|year=2013 | |year=2013 | ||
|journal=Mitochondrion | |journal=Mitochondrion |
Revision as of 09:38, 9 June 2015
SjΓΆvall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, ArnbjΓΆrnsson E, Hansson Magnus J, Fellman V, ElmΓ©r E (2013) Mitochondrial respiration in human viable platelets - methodology and influence of gender, age and storage. Mitochondrion 13:7-14. |
Sjoevall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjoernsson E, Hansson Magnus J, Fellman V, Elmer E (2013) Mitochondrion
Abstract: Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability. β’ Keywords: Diagnostics; Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders
β’ O2k-Network Lab: SE Lund Elmer E
Labels: MiParea: Respiration, mt-Medicine, Patients
Pathology: Aging;senescence, Neurodegenerative
Stress:Ischemia-reperfusion;preservation"Ischemia-reperfusion;preservation" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.
Organism: Human
Tissue;cell: Blood cells
Preparation: Intact cells, Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
O2k-Publications
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