Sjoevall 2013 Mitochondrion: Difference between revisions
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|abstract=Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ''ex-vivo'' analysis with the need for minute sample amounts and excellent reproducibility and stability. | |abstract=Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ''ex-vivo'' analysis with the need for minute sample amounts and excellent reproducibility and stability. | ||
|keywords=Diagnostics; Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders | |keywords=Diagnostics; Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders | ||
|mipnetlab=SE Lund Elmer E | |mipnetlab=SE Lund Elmer E, JP Tokyo Uchino H | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Medicine, Patients | |area=Respiration, Instruments;methods, mt-Medicine, Patients | ||
|diseases=Aging;senescence, Neurodegenerative | |||
|injuries=Ischemia-reperfusion | |||
|organism=Human | |organism=Human | ||
|tissues=Blood cells | |tissues=Blood cells, Platelet | ||
|preparations=Permeabilized cells, Intact cells | |||
|preparations= | |couplingstates=LEAK, ROUTINE, OXPHOS, ET | ||
|pathways=N, ROX | |||
|couplingstates=LEAK, ROUTINE, OXPHOS, | |||
| | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=SE, JP, MitoEAGLE blood cells data | ||
}} | }} | ||
== O2k-Publications == | == O2k-Publications == | ||
*[[O2k-Publications: Blood cells]] | *[[O2k-Publications: Blood cells]] | ||
*[[O2k-Publications: | *[[O2k-Publications: Platelets]] | ||
>>[[Bioblast_alert_2014]](02) | >>[[Bioblast_alert_2014]](02) |
Latest revision as of 23:05, 29 February 2020
SjΓΆvall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, ArnbjΓΆrnsson E, Hansson Magnus J, Fellman V, ElmΓ©r E (2013) Mitochondrial respiration in human viable platelets - methodology and influence of gender, age and storage. Mitochondrion 13:7-14. |
Sjoevall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjoernsson E, Hansson Magnus J, Fellman V, Elmer E (2013) Mitochondrion
Abstract: Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability. β’ Keywords: Diagnostics; Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders
β’ O2k-Network Lab: SE Lund Elmer E, JP Tokyo Uchino H
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine, Patients
Pathology: Aging;senescence, Neurodegenerative
Stress:Ischemia-reperfusion
Organism: Human
Tissue;cell: Blood cells, Platelet
Preparation: Permeabilized cells, Intact cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, ROX
HRR: Oxygraph-2k
SE, JP, MitoEAGLE blood cells data
O2k-Publications
>>Bioblast_alert_2014(02)