Severina 2007 Biochim Biophys Acta: Difference between revisions
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{{Publication | {{Publication | ||
|title=Severina II, Vyssokikh MY, Pustovidko AV, Simonyan RA, Rokitskaya TI, Skulachev VP (2007) Effects of lipophilic dications on planar bilayer phospholipid membrane and mitochondria. Biochim Biophys Acta. 1767: 1164-1168. | |title=Severina II, Vyssokikh MY, Pustovidko AV, Simonyan RA, Rokitskaya TI, Skulachev VP (2007) Effects of lipophilic dications on planar bilayer phospholipid membrane and mitochondria. Biochim Biophys Acta. 1767: 1164-1168. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/17692814 PMID: 17692814] | |||
|authors=Severina II, Vyssokikh MY, Pustovidko AV, Simonyan RA, Rokitskaya TI, Skulachev VP | |authors=Severina II, Vyssokikh MY, Pustovidko AV, Simonyan RA, Rokitskaya TI, Skulachev VP | ||
|year=2007 | |year=2007 | ||
|journal=Biochim. Biophys. Acta | |journal=Biochim. Biophys. Acta | ||
|abstract=In this paper, we studied effects of phosphonium dications P2C5 and P2C10 on bilayer planar phospholipid membrane (BLM) and rat liver mitochondria. In line with our previous observations [M.F. Ross, T. Da Ros, F.H. Blaikie, T.A. Prime, C.M. Porteous, I.I. Severina, V.P. Skulachev, H.G. Kjaergaard, R.A. Smith, M.P. Murphy, Accumulation of lipophilic dications by mitochondria and cells, Biochem. J. 400 (2006) 199โ208], we showed both P2C5 and P2C10 are cationic penetrants for BLM. They generated transmembrane diffusion potential (ฮฮจ), the compartment with a lower dication concentration positive. However, the ฮฮจ values measured proved to be lower that the Nernstian. This fact could be explained by rather low BLM conductance for the cations at their small concentrations and by induction of some BLM damage at their large concentrations. The damage in question consisted in appearance of non-Ohmic current/voltage relationships which increased in time. Such a non-Ohmicity was especially strong at ฮฮจ > 100 mV. Addition of penetrating lipophilic anion TPB, which increases the BLM conductance for lipophilic cations, yielded the Nernstian ฮฮจ, i.e. 30 mV per ten-fold dication gradient. In the State 4 mitochondria, dications stimulated respiration and lowered ฮฮจ. Moreover, they inhibited the State 3 respiration with succinate or glutamate and malate (but not with TMPD and ascorbate) in an uncoupler-sensitive fashion. Effect on the in State 4 mitochondria, similarly to that on BLM, was accounted for by a time-dependent membrane damage. On the other hand, the State 3 effect was most probably due to inhibition of the respiratory chain Complex I and/or Complex III. The damaging and inhibitory activities of lipophilic dications should be taken into account when one considers a possibility to use them as a vehicle to target antioxidants or other compounds to mitochondria. | |abstract=In this paper, we studied effects of phosphonium dications P2C5 and P2C10 on bilayer planar phospholipid membrane (BLM) and rat liver mitochondria. In line with our previous observations [M.F. Ross, T. Da Ros, F.H. Blaikie, T.A. Prime, C.M. Porteous, I.I. Severina, V.P. Skulachev, H.G. Kjaergaard, R.A. Smith, M.P. Murphy, Accumulation of lipophilic dications by mitochondria and cells, Biochem. J. 400 (2006) 199โ208], we showed both P2C5 and P2C10 are cationic penetrants for BLM. They generated transmembrane diffusion potential (ฮฮจ), the compartment with a lower dication concentration positive. However, the ฮฮจ values measured proved to be lower that the Nernstian. This fact could be explained by rather low BLM conductance for the cations at their small concentrations and by induction of some BLM damage at their large concentrations. The damage in question consisted in appearance of non-Ohmic current/voltage relationships which increased in time. Such a non-Ohmicity was especially strong at ฮฮจ > 100 mV. Addition of penetrating lipophilic anion TPB, which increases the BLM conductance for lipophilic cations, yielded the Nernstian ฮฮจ, i.e. 30 mV per ten-fold dication gradient. In the State 4 mitochondria, dications stimulated respiration and lowered ฮฮจ. Moreover, they inhibited the State 3 respiration with succinate or glutamate and malate (but not with TMPD and ascorbate) in an uncoupler-sensitive fashion. Effect on the in State 4 mitochondria, similarly to that on BLM, was accounted for by a time-dependent membrane damage. On the other hand, the State 3 effect was most probably due to inhibition of the respiratory chain Complex I and/or Complex III. The damaging and inhibitory activities of lipophilic dications should be taken into account when one considers a possibility to use them as a vehicle to target antioxidants or other compounds to mitochondria. | ||
|keywords=Dication,ย | |keywords=Dication,ย lipophilic cation, planar phospholipids membrane,ย mitochondria, membrane potential, damaging effect | ||
| | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |instruments=Oxygraph-2k | ||
|injuries=RONS; Oxidative Stress | |injuries=RONS; Oxidative Stress | ||
|organism=Rat | |organism=Rat | ||
|tissues=Hepatocyte; Liver | |tissues=Hepatocyte; Liver | ||
|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria | ||
|topics=Coupling; Membrane Potential | |||
|topics= | |discipline=Mitochondrial Physiology | ||
| | |||
}} | }} | ||
Revision as of 14:19, 7 September 2011
ยป [[Has info::PMID: 17692814]]
Was written by::Severina II, Was written by::Vyssokikh MY, Was written by::Pustovidko AV, Was written by::Simonyan RA, Was written by::Rokitskaya TI, Was written by::Skulachev VP (Was published in year::2007) Was published in journal::Biochim. Biophys. Acta
Abstract: [[has abstract::In this paper, we studied effects of phosphonium dications P2C5 and P2C10 on bilayer planar phospholipid membrane (BLM) and rat liver mitochondria. In line with our previous observations [M.F. Ross, T. Da Ros, F.H. Blaikie, T.A. Prime, C.M. Porteous, I.I. Severina, V.P. Skulachev, H.G. Kjaergaard, R.A. Smith, M.P. Murphy, Accumulation of lipophilic dications by mitochondria and cells, Biochem. J. 400 (2006) 199โ208], we showed both P2C5 and P2C10 are cationic penetrants for BLM. They generated transmembrane diffusion potential (ฮฮจ), the compartment with a lower dication concentration positive. However, the ฮฮจ values measured proved to be lower that the Nernstian. This fact could be explained by rather low BLM conductance for the cations at their small concentrations and by induction of some BLM damage at their large concentrations. The damage in question consisted in appearance of non-Ohmic current/voltage relationships which increased in time. Such a non-Ohmicity was especially strong at ฮฮจ > 100 mV. Addition of penetrating lipophilic anion TPB, which increases the BLM conductance for lipophilic cations, yielded the Nernstian ฮฮจ, i.e. 30 mV per ten-fold dication gradient. In the State 4 mitochondria, dications stimulated respiration and lowered ฮฮจ. Moreover, they inhibited the State 3 respiration with succinate or glutamate and malate (but not with TMPD and ascorbate) in an uncoupler-sensitive fashion. Effect on the in State 4 mitochondria, similarly to that on BLM, was accounted for by a time-dependent membrane damage. On the other hand, the State 3 effect was most probably due to inhibition of the respiratory chain Complex I and/or Complex III. The damaging and inhibitory activities of lipophilic dications should be taken into account when one considers a possibility to use them as a vehicle to target antioxidants or other compounds to mitochondria.]] โข Keywords: has publicationkeywords::Dication, has publicationkeywords::lipophilic cation, has publicationkeywords::planar phospholipids membrane, has publicationkeywords::mitochondria, has publicationkeywords::membrane potential, has publicationkeywords::damaging effect
Labels:
Stress:Injury and adaptation::RONS; Oxidative Stress Organism: Organism::Rat Tissue;cell: tissue and cell::Hepatocyte; Liver Preparation: Preparation::Isolated Mitochondria
Regulation: Topic::Coupling; Membrane Potential
HRR: Instrument and method::Oxygraph-2k
