Scheibye-Knudsen 2009 Eur J Appl Physiol
| Scheibye-Knudsen M, Quistorff B (2009) Regulation of mitochondrial respiration by inorganic phosphate: comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle. Eur J Appl Physiol 105: 279-287. |
Scheibye-Knudsen M, Quistorff B (2009) Eur. J. Appl. Physiol.
Abstract: ADP is generally accepted as a key regulator of oxygen consumption both in isolated mitochondria and in permeabilized fibers from skeletal muscle. The present study explored inorganic phosphate in a similar regulatory role. Saponin permeabilized fibers and isolated mitochondria from type-I and type-II muscle from male Wistar rats were prepared. Respiration was measured while the medium Pi concentration was gradually increased. The apparent Km values for Pi were 607 Β± 17 Β΅M and 405 Β± 15 ΞΌM (P < 0.0001) for type-I and type-II fibers, respectively. For isolated mitochondria the values were significantly lower than type-1 permeabilized fibers, 338 Β± 130 ΞΌM and 235 Β± 30 ΞΌM (P < 0.05), but not different with respect to fiber type. The reason for this difference in Km values in the permeabilized muscle is unknown, but a similar pattern has been observed for K m of ADP. Our data indicate that phosphate may play a role in regulation of oxygen consumption in vitro and in vivo. β’ Keywords: Mitochondrial respiration, Permeabilized muscle, Inorganic phosphate, Striated muscle, Oxygen consumption, Oxidative phosphorylation
β’ O2k-Network Lab: DK Copenhagen Quistorff B
Labels:
Organism: Rat
Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Permeabilized Tissue"Permeabilized Tissue" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
