Scheibye-Knudsen 2009 Eur J Appl Physiol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Scheibye-Knudsen M, Quistorff B (2009) Regulation of mitochondrial respiration by inorganic phosphate: comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle. Eur | |title=Scheibye-Knudsen M, Quistorff B (2009) Regulation of mitochondrial respiration by inorganic phosphate: comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle. Eur J Appl Physiol 105:279-87. | ||
|authors=Scheibye-Knudsen M, Quistorff B ย | |info=[http://www.ncbi.nlm.nih.gov/pubmed/18989695 PMID: 18989695] | ||
|authors=Scheibye-Knudsen M, Quistorff B | |||
|year=2009 | |year=2009 | ||
|journal=Eur | |journal=Eur J Appl Physiol | ||
| | |abstract=ADP is generally accepted as a key regulator of oxygen consumption both in isolated mitochondria and in permeabilized fibers from skeletal muscle. The present study explored inorganic phosphate in a similar regulatory role. Saponin permeabilized fibers and isolated mitochondria from type-I and type-II muscle from male Wistar rats were prepared. Respiration was measured while the medium Pi concentration was gradually increased. The apparent Km values for Pi were 607 ยฑ 17 ยตM and 405 ยฑ 15 ฮผM (P < 0.0001) for type-I and type-II fibers, respectively. For isolated mitochondria the values were significantly lower than type-1 permeabilized fibers, 338 ยฑ 130 ฮผM and 235 ยฑ 30 ฮผM (P < 0.05), but not different with respect to fiber type. The reason for this difference in Km values in the permeabilized muscle is unknown, but a similar pattern has been observed for Kย m of ADP. Our data indicate that phosphate may play a role in regulation of oxygen consumption ''in vitro'' and ''in vivo''. | ||
|keywords=Mitochondrial respiration, Permeabilized muscle, Inorganic phosphate, Striated muscle, Oxygen consumption, Oxidative phosphorylation | |||
|mipnetlab=DK Copenhagen Quistorff B | |||
|discipline=Mitochondrial Physiology | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |||
|tissues=Skeletal muscle | |||
|preparations=Permeabilized tissue, Isolated mitochondria | |||
|topics=Phosphate | |||
|couplingstates=OXPHOS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
| | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Latest revision as of 11:31, 15 June 2015
| Scheibye-Knudsen M, Quistorff B (2009) Regulation of mitochondrial respiration by inorganic phosphate: comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle. Eur J Appl Physiol 105:279-87. |
Scheibye-Knudsen M, Quistorff B (2009) Eur J Appl Physiol
Abstract: ADP is generally accepted as a key regulator of oxygen consumption both in isolated mitochondria and in permeabilized fibers from skeletal muscle. The present study explored inorganic phosphate in a similar regulatory role. Saponin permeabilized fibers and isolated mitochondria from type-I and type-II muscle from male Wistar rats were prepared. Respiration was measured while the medium Pi concentration was gradually increased. The apparent Km values for Pi were 607 ยฑ 17 ยตM and 405 ยฑ 15 ฮผM (P < 0.0001) for type-I and type-II fibers, respectively. For isolated mitochondria the values were significantly lower than type-1 permeabilized fibers, 338 ยฑ 130 ฮผM and 235 ยฑ 30 ฮผM (P < 0.05), but not different with respect to fiber type. The reason for this difference in Km values in the permeabilized muscle is unknown, but a similar pattern has been observed for K m of ADP. Our data indicate that phosphate may play a role in regulation of oxygen consumption in vitro and in vivo. โข Keywords: Mitochondrial respiration, Permeabilized muscle, Inorganic phosphate, Striated muscle, Oxygen consumption, Oxidative phosphorylation
โข O2k-Network Lab: DK Copenhagen Quistorff B
Labels:
Organism: Rat
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue, Isolated mitochondria
Regulation: Phosphate Coupling state: OXPHOS
HRR: Oxygraph-2k
