Saraiva 2022 Pathogens: Difference between revisions
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|keywords=ROS, Trypanosoma cruzi, Bioenergetics, Hypoxia, Parasite metabolism | |keywords=ROS, Trypanosoma cruzi, Bioenergetics, Hypoxia, Parasite metabolism | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
|mipnetlab=BR Rio de Janeiro Paes MC | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
|injuries=Hypoxia | |||
|organism=Protists | |||
|preparations=Permeabilized cells | |||
|couplingstates=LEAK, OXPHOS | |||
|pathways=S, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2022-11 | |additional=2022-11 | ||
}} | }} |
Latest revision as of 13:27, 22 November 2022
[[Has title::Saraiva FMS, Cosentino-Gomes D, Inacio JDF, Almeida-Amaral EE, Louzada-Neto O, Rossini A, Nogueira NP, Meyer-Fernandes JR, Paes MC (2022) Hypoxia effects on Trypanosoma cruzi epimastigotes proliferation, differentiation, and energy metabolism. https://doi.org/10.3390/pathogens11080897]] |
ยป [[Has info::Pathogens 11:897. PMID: 36015018 Open Access]]
Was written by::Saraiva Francis MS, Was written by:: Cosentino-Gomes Daniela, Was written by:: Inacio Job DF, Was written by::Almeida-Amaral Elmo E, Was written by:: Louzada-Neto Orlando, Was written by::Rossini Ana, Was written by:: Nogueira Natalia P, Was written by:: Meyer-Fernandes Jose R, Was written by:: Paes Marcia C (Was published in year::2022) Was published in journal::Pathogens
Abstract: [[has abstract::Trypanosoma cruzi, the causative agent of Chagas disease, faces changes in redox status and nutritional availability during its life cycle. However, the influence of oxygen fluctuation upon the biology of T. cruzi is unclear. The present work investigated the response of T. cruzi epimastigotes to hypoxia. The parasites showed an adaptation to the hypoxic condition, presenting an increase in proliferation and a reduction in metacyclogenesis. Additionally, parasites cultured in hypoxia produced more reactive oxygen species (ROS) compared to parasites cultured in normoxia. The analyses of the mitochondrial physiology demonstrated that hypoxic condition induced a decrease in both oxidative phosphorylation and mitochondrial membrane potential (ฮฮจm) in epimastigotes. In spite of that, ATP levels of parasites cultivated in hypoxia increased. The hypoxic condition also increased the expression of the hexokinase and NADH fumarate reductase genes and reduced NAD(P)H, suggesting that this increase in ATP levels of hypoxia-challenged parasites was a consequence of increased glycolysis and fermentation pathways. Taken together, our results suggest that decreased oxygen levels trigger a shift in the bioenergetic metabolism of T. cruzi epimastigotes, favoring ROS production and fermentation to sustain ATP production, allowing the parasite to survive and proliferate in the insect vector.]] โข Keywords: has publicationkeywords::ROS, has publicationkeywords::Trypanosoma cruzi, has publicationkeywords::Bioenergetics, has publicationkeywords::Hypoxia, has publicationkeywords::Parasite metabolism โข Bioblast editor: [[has editor::Plangger M]] โข O2k-Network Lab: Was published by MiPNetLab::BR Rio de Janeiro Paes MC
Labels: MiParea: MiP area::Respiration
Stress:Injury and adaptation::Hypoxia Organism: Organism::Protists
Preparation: Preparation::Permeabilized cells
Coupling state: Coupling states::LEAK, Coupling states::OXPHOS
Pathway: Pathways::S, Pathways::ROX
HRR: Instrument and method::Oxygraph-2k