Difference between revisions of "Sahl 2021 Adipocyte"
|Line 12:||Line 12:|
Revision as of 18:55, 11 November 2021
|Sahl RE, Høy Helms EF, Schmücker M, Flensted-Jensen M, Ingersen A, Morville T, Dela F, Helge JW, Larsen S (2021) Reliability and variation in mitochondrial respiration in human adipose tissue. Adipocyte [Epub ahead of print].|
Abstract: Adipose tissue mitochondrial function is gaining increasing interest since it is good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue decline in a gradual manner when analyses are postponed 3h and 24h, with a statistically significant decline 24h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggest that the optimal amount of tissue to be used is 20mg and that different technicians handling the biopsy do not affect MRC. • Keywords: Adipose tissue, Coefficient of Variation, High resolution respirometry, Human, Mitochondria • Bioblast editor: Plangger M • O2k-Network Lab: DK Copenhagen Larsen S
Labels: MiParea: Respiration
Organism: Human Tissue;cell: Fat Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ET Pathway: N, NS HRR: Oxygraph-2k