SUIT-022: Difference between revisions
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|info='''A: | |info='''A: Determination of the respiration due to the alternative oxidase pathway''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-022.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-022.pdf Versions] | ||
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== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::*[[SUIT-023 O2 ce D053]] for microalgal intact cells | |||
== References == | == References == |
Revision as of 11:13, 9 April 2019
Description
Reference: A: Determination of the respiration due to the alternative oxidase pathway ยปVersions
O2k-Application: O2
- SUIT-category: ce
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the alternative oxidase pathway, which is cyanide-and antimycin A-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see SUIT-023).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Specific SUIT protocols
SUIT-022 O2 ce D051
- SUIT-022 O2 ce D051 for intact microalgal cells (ce)
Strengths and limitations
- SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the contribution of the cytochrome c pathway to respiration, it must be carried out in parallel to SUIT-023.
- + This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-023 O2 ce D053 for microalgal intact cells
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry