Difference between revisions of "SUIT-010"
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::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceS,S | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceS,S | ||
::: '''[[SUIT protocol pattern]]:''' ce1;ce2Rot;ce3S;ce4D;1Dig;1c | ::: '''[[SUIT protocol pattern]]:''' ce1;ce2Rot;ce3S;ce4D;1Dig;1c | ||
SUIT-010 is designed for evaluation of optimum [[digitonin]] concentration for permeabilizing cells, a requirement to account for differendes between cell types, concentration of cells, and variability between batches of the natural product [[digitonin]]. | |||
__TOC__ | __TOC__ | ||
Communicated by [[Cardoso LH]], [[Iglesias-Gonzalez J]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-01-29) | Communicated by [[Cardoso LH]], [[Iglesias-Gonzalez J]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-01-29) |
Revision as of 09:40, 5 February 2019
Description
Abbreviation: ceS,S
Reference: A Optimization of digitonin concentration for pce »Versions
- SUIT-category: ceS,S
- SUIT protocol pattern: ce1;ce2Rot;ce3S;ce4D;1Dig;1c
SUIT-010 is designed for evaluation of optimum digitonin concentration for permeabilizing cells, a requirement to account for differendes between cell types, concentration of cells, and variability between batches of the natural product digitonin.
Communicated by Cardoso LH, Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-01-29)
Specific SUIT protocols
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | Human Mouse Rat Saccharomyces cerevisiae | Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2Rot | ROX | ce1;ce2Rot
| ||
ce3S | SL(n) | S | CII | ce1;ce2Rot;ce3S
|
ce4D | SP | S | CII | ce1;ce2Rot;ce3S;ce4D
|
1Dig | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig
|
1c | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig;1c
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Digitonin is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digitonin concentrations for complete plasma membrane permeabilization can be determined with this protocol.
Compare SUIT protocols
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry