SUIT-010: Difference between revisions
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::: '''[[SUIT protocol pattern]]:''' ce1;ce2Rot;ce3S;ce4D;1Dig;1c | ::: '''[[SUIT protocol pattern]]:''' ce1;ce2Rot;ce3S;ce4D;1Dig;1c | ||
__TOC__ | __TOC__ | ||
Β | Communicated by [[Cardoso LH]] [[Iglesias-Gonzalez J]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-01-21) | ||
== DLP applications == | == DLP applications == | ||
::::* [[SUIT-010 O2 pce D08]] | ::::* [[SUIT-010 O2 pce D08]] |
Revision as of 17:25, 22 January 2019
Description
Abbreviation: ceS,S
Reference: A Optimum digitonin concentration for pce Β»Versions
- SUIT-category: ceS,S
- SUIT protocol pattern: ce1;ce2Rot;ce3S;ce4D;1Dig;1c
Communicated by Cardoso LH Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-01-21)
DLP applications
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, MΓ©szΓ‘ros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | Human Mouse Rat Saccharomyces cerevisiae | Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2Rot | ROX | ce1;ce2Rot
| ||
ce3S | SL(n) | S | CII | ce1;ce2Rot;ce3S
|
ce4D | SP | S | CII | ce1;ce2Rot;ce3S;ce4D
|
1Dig | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig
|
1c | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig;1c
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Digitonin is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digitonin concentrations for complete plasma membrane permeabilization can be determined with this protocol.
Compare SUIT protocols
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry