SUIT-004: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=NS(PM) | |abbr=NS(PM) | ||
|description=[[File:1PM;2D | |description=[[File:1PM;2D;3U;4S;5Rot-.png|300px]] | ||
|info='''A: short RP1''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-004.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-004.pdf Versions] | |info='''A: short RP1''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-004.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-004.pdf Versions] | ||
}} | }} |
Revision as of 18:00, 24 January 2019
Description
Abbreviation: NS(PM)
Reference: A: short RP1 ยปVersions
- SUIT-category: NS(PM)
- SUIT protocol pattern: orthogonal 1PM;2D;2c;3U;4S;5Rot;6Ama
Communicated by Doerrier C, Iglesias-Gonzalez J and Gnaiger E (last update 2019-01-21)
DLP applications
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1PM | PML(n) | N | CI | 1PM
|
2D | PMP | N | CI | 1PM;2D
|
2c | PMcP | N | CI | 1PM;2D;2c
|
3U | PME | N | CI | 1PM;2D;3U
|
4S | PMSE | NS | CI&II | 1PM;2D;3U;4S
|
5Rot | SE | S | CII | 1PM;2D;3U;4S;5Rot
|
6Ama | ROX | 1PM;2D;3U;4S;5Rot;6Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- A succinate concentration of >10 mM may be required for saturating SE capacity.
- + Linear coupling control (L-P-E) with N substrates (PM).
- + Pathway control in ET state (N, NS and S pathways).
- + This protocol can be extended with the Complex IV module.
- + Short experimental time.
- - The substrate combination for maximum ET capacity (FNSGP) is not obtained in SUIT-004 in favor of reducing the experimental time.
Compare SUIT protocols
- SUIT-001 (RP1): SUIT-004 is a shortended version of RP1; without G, F and Gp.
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry