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SUIT-003 O2 ce D028

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high-resolution terminology - matching measurements at high-resolution


SUIT-003 O2 ce D028

Description

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Abbreviation: CCP_R 1Omy 2U 3Rot 4S 5Ama


MitoPedia concepts: SUIT protocol 

SUIT-catg: CCP(S)
SUIT protocol pattern: linear coupling control protocol (CCP)
Mark names in DatLab: CCP(S)R2
DatLab-Excel template: in prep.: CCP(S)R2.xlsx
  • 3Rot 4S 5Ama
3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP_02a.
4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).