- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
SUIT-catg: CCPS
Abbreviation: CCP_R 1Omy 2U 3Rot 4S 5Ama
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP_R1
- DatLab-Excel template: in prep.: CCP_R1.xlsx
- 3Rot 4S 5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP02; compare CCP01, Ce_R 1Omy 2U 3Rot).
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
