SUIT-003 O2 ce D028: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=CCP(S)04_R,1Omy,2U-3Rot,4S,5Ama | ||
|description=[[File:CCP(S)04.jpg|200px]] | |description=[[File:CCP(S)04.jpg|200px]] | ||
|info='''A''' CCP_RP2 | |info='''A''' CCP_RP2 | ||
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:::* '''3Rot,4S-5Ama''' | :::* '''3Rot,4S-5Ama''' | ||
:::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[ | :::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[CCP02|CCP02-Rot,Ama]]. | ||
:::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | :::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | ||
:::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). |
Revision as of 01:50, 21 September 2016
Description
Abbreviation: CCP(S)04_R,1Omy,2U-3Rot,4S,5Ama
Reference: A CCP_RP2
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)R2
- DatLab-Analysis template: in prep.: CCP(S)R2.xlsx
R,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 3Rot,4S-5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP02-Rot,Ama.
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).