Difference between revisions of "SUIT-003 O2 ce D028"
From Bioblast
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|application=O2 | |application=O2 | ||
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{{MitoPedia concepts | |||
|mitopedia concept=SUIT protocol, Recommended | |||
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{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
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{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics}} | |||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceS | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceS | ||
::: '''[[SUIT protocol pattern]]:''' linear cell [[coupling control protocol]] ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama | ::: '''[[SUIT protocol pattern]]:''' linear cell [[coupling control protocol]] ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama | ||
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:::: '''ce5S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated. | :::: '''ce5S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated. | ||
:::: '''ce6Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''ce6Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 15:42, 15 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
Abbreviation: ceS
Reference: A CCP(S)
O2k-Application: O2
MitoPedia concepts:
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry
- SUIT-category: ceS
- SUIT protocol pattern: linear cell coupling control protocol ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama
ceR,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- ce4Rot,ce5S;ce6Ama
- ce4Rot: In many intact cells ROX measured after titration of rotenone (ce4Rot) is not or only slightly further inhibited by subsequent titration of antimycin A.
- ce5S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
- ce6Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
