Difference between revisions of "SUIT-003 O2 ce D028"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=RLE(S) | |abbr=RLE(S) | ||
|description=[[File: | |description=[[File:1ce;2ceOmy;3ceU;4ceRot;5ceS;6ceAma.jpg|200px]] | ||
|info='''A''' CCP(S) | |info='''A''' CCP(S) | ||
}} | }} | ||
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}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceRLE(S) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceRLE(S) | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] ( | ::: '''[[SUIT protocol pattern]]:''' linear cell [[coupling control protocol]] (ceCCP) | ||
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[[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states. | [[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states. | ||
:::* ''' | :::* '''4ceRot,5ceS;6ceAma''' | ||
:::: ''' | :::: '''4ceRot''': In many intact cells ROX measured after titration of rotenone (4ceRot) is not or only slightly further inhibited by subsequent titration of antimycin A. | ||
:::: ''' | :::: '''5ceS''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated. | ||
:::: ''' | :::: '''6ceAma''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 08:57, 5 August 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
Abbreviation: RLE(S)
Reference: A CCP(S)
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: ceRLE(S)
- SUIT protocol pattern: linear cell coupling control protocol (ceCCP)
ceR,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 4ceRot,5ceS;6ceAma
- 4ceRot: In many intact cells ROX measured after titration of rotenone (4ceRot) is not or only slightly further inhibited by subsequent titration of antimycin A.
- 5ceS: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
- 6ceAma: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
