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Difference between revisions of "SUIT-003 O2 ce D028"

From Bioblast
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|abbr=RLE(S)
|abbr=RLE(S)
|description=[[File:CCP(S)04.jpg|200px]]
|description=[[File:CCP(S)04.jpg|200px]]
|info='''A''' CCP(S)_RP2
|info='''A''' CCP(S)
}}
}}
{{MitoPedia concepts
{{MitoPedia concepts
|mitopedia concept=SUIT protocol, SUIT A
|mitopedia concept=SUIT protocol, SUIT A
}}
}}
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' RLE(S)
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceRLE(S)
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP)
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP)






== R,L,E format ==
== ceR,L,E format ==
[[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
[[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.


:::* '''3Rot,4S;5Ama''' Β 
:::* '''3ceRot,4ceS;5ceAma''' Β 
:::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[1R;2Omy;3U;4Rot;5S;6Ama]]).
:::: '''3ceRot''': In many intact cells ROX measured after titration of rotenone (3ceRot) is not or only slightly further inhibited by subsequent titration of antimycin A.
:::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
:::: '''4ceS''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
:::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]).
:::: '''5ceAma''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]).

Revision as of 23:33, 4 August 2017


high-resolution terminology - matching measurements at high-resolution


SUIT-003 O2 ce D028

Description

Ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama.png

Abbreviation: RLE(S)

Reference: A CCP(S)


MitoPedia concepts: SUIT protocol, SUIT A 

SUIT-category: ceRLE(S)
SUIT protocol pattern: linear coupling control protocol (CCP)


ceR,L,E format

R;L;E format.jpg In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.

  • 3ceRot,4ceS;5ceAma
3ceRot: In many intact cells ROX measured after titration of rotenone (3ceRot) is not or only slightly further inhibited by subsequent titration of antimycin A.
4ceS: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
5ceAma: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).