Difference between revisions of "SUIT-003 O2 ce D028"
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m (Gnaiger Erich moved page 1R;2Omy;3U;4Rot;5S;6Ama to 1ce;2Omy;3U;4Rot;5S;6Ama) |
m (Gnaiger Erich moved page 1ce;2Omy;3U;4Rot;5S;6Ama to 1ce;2ceOmy;3ceU;4ceRot;5ceS;6ceAma) |
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Revision as of 23:26, 4 August 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
Abbreviation: RLE(S)
Reference: A CCP(S)_RP2
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: RLE(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
R,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 3Rot,4S;5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (1R;2Omy;3U;4Rot;5S;6Ama).
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
