Difference between revisions of "SUIT-003 O2 ce D028"
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{{MitoPedia | {{MitoPedia | ||
|abbr=CCP_R 1Omy 2U 3Rot 4S 5Ama | |abbr=CCP_R,1Omy,2U,3Rot,4S-5Ama | ||
|description=[[File:CCP_R 1Omy 2U 3Rot 4S 5Ama.jpg|200px]] | |description=[[File:CCP_R 1Omy 2U 3Rot 4S 5Ama.jpg|200px]] | ||
|info= | |info=CCP_R2 | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT protocol | |mitopedia concept=SUIT protocol, SUIT A | ||
}} | }} | ||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics}} | |||
::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ||
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::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': CCP(S)R2.xlsx | ::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': CCP(S)R2.xlsx | ||
:::* '''3Rot 4S 5Ama''' | :::* '''3Rot,4S-5Ama''' | ||
:::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[CCP_02|CCP_02a]]. | :::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[CCP_02|CCP_02a]]. | ||
:::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | :::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | ||
:::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 19:52, 17 September 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
Abbreviation: CCP_R,1Omy,2U,3Rot,4S-5Ama
Reference: CCP_R2
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)R2
- DatLab-Excel template: in prep.: CCP(S)R2.xlsx
- 3Rot,4S-5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP_02a.
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
