SUIT-003 O2 ce D009: Difference between revisions
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:::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | :::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ||
:::* '''Rot and Ama''' | |||
:::: In this CCP, ROX is measured after titration of [[rotenone]], [[antimycin A]], or both in a single step. Validity of using [[rotenone]] only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama). | |||
::::ยป ''Further details'': [[CCP Huetter 2004 Biochem J]], Ce_R 1Omy 2U 3Rot 4Ama |
Revision as of 07:42, 27 August 2016
Description
SUIT-catg: Ce
Mark names in DatLab: MiPNet08_09_CellRespiration
DatLab-Excel template: SUIT_MiPNet08.09_CellRespiration.xlsx
Abbreviation: Ce_R 1Omy 2U 3Ama
Reference: MiPNet08.09 CellRespiration
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Rot and Ama
- In this CCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama).
- ยป Further details: CCP Huetter 2004 Biochem J, Ce_R 1Omy 2U 3Rot 4Ama