SUIT-003 O2 ce D009: Difference between revisions

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:::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP)
:::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP)
:::* '''Rot and Ama'''
:::: In this CCP, ROX is measured after titration of [[rotenone]], [[antimycin A]], or both in a single step. Validity of using [[rotenone]] only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama).
::::ยป ''Further details'': [[CCP Huetter 2004 Biochem J]], Ce_R 1Omy 2U 3Rot 4Ama

Revision as of 07:42, 27 August 2016


high-resolution terminology - matching measurements at high-resolution


SUIT-003 O2 ce D009

Description

SUIT-catg: Ce

Mark names in DatLab: MiPNet08_09_CellRespiration

DatLab-Excel template: SUIT_MiPNet08.09_CellRespiration.xlsx

Abbreviation: Ce_R 1Omy 2U 3Ama

Reference: MiPNet08.09 CellRespiration


MitoPedia concepts: SUIT protocol 

SUIT protocol pattern: linear coupling control protocol (CCP)
  • Rot and Ama
In this CCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama).
ยป Further details: CCP Huetter 2004 Biochem J, Ce_R 1Omy 2U 3Rot 4Ama
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