Difference between revisions of "Ryan 2016 J Mol Cell Cardiol"
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{{Publication | {{Publication | ||
|title=Ryan TE, Schmidt CA, Alleman RJ, Tsang AM, Green TD, Neufer PD, Brown DA, McClung JM (2016) Mitochondrial therapy improves limb perfusion and myopathy following hindlimb ischemia. J Mol Cell Cardiol 97:191-6. ย | |title=Ryan TE, Schmidt CA, Alleman RJ, Tsang AM, Green TD, Neufer PD, Brown DA, McClung JM (2016) Mitochondrial therapy improves limb perfusion and myopathy following hindlimb ischemia. J Mol Cell Cardiol 97:191-6. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/27262673 PMID: 27262673] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/27262673 PMID: 27262673] | ||
|authors=Ryan TE, Schmidt CA, Alleman RJ, Tsang AM, Green TD, Neufer PD, Brown DA, McClung JM | |authors=Ryan TE, Schmidt CA, Alleman RJ, Tsang AM, Green TD, Neufer PD, Brown DA, McClung JM | ||
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Copyright ยฉ 2016. Published by Elsevier Ltd. | Copyright ยฉ 2016. Published by Elsevier Ltd. | ||
|keywords=Critical limb ischemia, Ischemia, Mitochondria, Peripheral artery disease | |keywords=Critical limb ischemia, Ischemia, Mitochondria, Peripheral artery disease | ||
|mipnetlab=US NC Greenville Neufer PD, US NC Greenville Brown DA | |mipnetlab=US NC Greenville Neufer PD, US NC Greenville Brown DA, US VA Blacksburg Brown DA | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Medicine | |area=Respiration, mt-Medicine | ||
|injuries=Ischemia-reperfusion | |||
|organism=Mouse | |organism=Mouse | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Isolated mitochondria | |preparations=Isolated mitochondria | ||
|couplingstates=LEAK, OXPHOS, ET | |||
|couplingstates=OXPHOS, | |pathways=N, S, CIV, NS, ROX | ||
| | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2016-07 | ||
}} | }} |
Latest revision as of 15:17, 28 March 2018
Ryan TE, Schmidt CA, Alleman RJ, Tsang AM, Green TD, Neufer PD, Brown DA, McClung JM (2016) Mitochondrial therapy improves limb perfusion and myopathy following hindlimb ischemia. J Mol Cell Cardiol 97:191-6. |
Ryan TE, Schmidt CA, Alleman RJ, Tsang AM, Green TD, Neufer PD, Brown DA, McClung JM (2016) J Mol Cell Cardiol
Abstract: Critical limb ischemia is a devastating manifestation of peripheral arterial disease with no effective strategies for improving morbidity and mortality outcomes. We tested the hypothesis that cellular mitochondrial function is a key component of limb pathology and that improving mitochondrial function represents a novel paradigm for therapy. BALB/c mice were treated with a therapeutic mitochondrial-targeting peptide (MTP-131) and subjected to limb ischemia (HLI). Compared to vehicle control, MTP-131 rescued limb muscle capillary density and blood flow (64.7ยฑ11% of contralateral vs. 39.9ยฑ4%), and improved muscle regeneration. MTP-131 also increased electron transport system flux across all conditions at HLI day-7. In vitro, primary muscle cells exposed to experimental ischemia demonstrated markedly reduced (~75%) cellular respiration, which was rescued by MTP-131 during a recovery period. Compared to muscle cells, endothelial cell (HUVEC) respiration was inherently protected from ischemia (~30% reduction), but was also enhanced by MTP-131. These findings demonstrate an important link between ischemic tissue bioenergetics and limb blood flow and indicate that the mitochondria may be a pharmaceutical target for therapeutic intervention during critical limb ischemia.
Copyright ยฉ 2016. Published by Elsevier Ltd. โข Keywords: Critical limb ischemia, Ischemia, Mitochondria, Peripheral artery disease
โข O2k-Network Lab: US NC Greenville Neufer PD, US NC Greenville Brown DA, US VA Blacksburg Brown DA
Labels: MiParea: Respiration, mt-Medicine
Stress:Ischemia-reperfusion Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k
2016-07