Ruthenium red: Difference between revisions

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Ruthenium red

Description

Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca²⁺ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca²⁺-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].

Abbreviation: RR

Reference: MiPNet03.02, Wikipedia

Application in HRR

RR: Ruthenium red (Ammoniated ruthenium oxychloride; (NH₃)₅RuORu(NH₃)₄ORu(NH₃)₅Cl₆), Sigma-Aldrich: R2751, store at RT, CAS: 11103-72-3, M = 786.35 g·mol^-1

Hazard statements: H302; Harmful if swallowed

Preparation of 2 mM stock solution (dissolved in H₂O) for 2-mL O2k-chamber:

Weigh 1.57 mg Ruthenium Red.
Dissolve in 1 mL H₂O.
Divide into 0.1 mL aliquots.
Store at -20 °C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
Final concentration: 1 µM.

Preparation of 0.5 mM stock solution (dissolved in H₂O) for 0.5-mL O2k-chamber:

Take 50 µL of the 1 mM stock solution.
Add 150 µL H₂O.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
Final concentration: 1 µM.

References

Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.

MitoPedia topics: Inhibitor

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|abbr=Rut
+|abbr=RR
-|description=Ruthenium red (ammoniated ruthenium oxychloride.
+|description='''Ruthenium red''' (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca<sup>2+</sup> uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca<sup>2+</sup>-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].
 |info=[[MiPNet03.02]], [http://en.wikipedia.org/wiki/Ruthenium_red Wikipedia]
 }}
+__TOC__
+== Application in [[HRR]] ==
+{{Chemical_description
+|abbr=RR
+|trivial name=Ruthenium red
+|complete name=Ammoniated ruthenium oxychloride
+|chem formula=(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>Cl<sub>6</sub>
+|molar mass=786.35
+|vendor=Sigma-Aldrich
+|product number=R2751
+|store at=RT
+|sensitivity=
+|cas=11103-72-3
+|h statements=H302
+|h info=Harmful if swallowed
+}}<!--:::'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>);   Sigma R 2751, 1 g; M = 786.35 g·mol<sup>-1</sup>.-->
+::::'''Preparation of 2 mM stock solution''' (dissolved in H<sub>2</sub>O)  for '''2-mL O2k-chamber''':
+::::# Weigh 1.57 mg Ruthenium Red.
+::::# Dissolve in 1 mL H<sub>2</sub>O.
+::::# Divide into 0.1 mL aliquots.
+::::# Store at -20 °C.
+:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
+::::* Final concentration: 1 µM.
+:::: '''Preparation of 0.5 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''0.5-mL O2k-chamber''':
+::::# Take 50 µL of the 1 mM stock solution.
+::::# Add 150 µL H<sub>2</sub>O.
+:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
+::::* Final concentration: 1 µM.
+== References ==
+# Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
+# Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
+{{MitoPedia methods}}
 {{MitoPedia topics
 |mitopedia topic=Inhibitor
 }}