Ruthenium red: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | |||
|abbr=Rut | |||
|trivial name=Ruthenium red | |||
|complete name=Ammoniated ruthenium oxychloride | |||
|chem formula=(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>Cl<sub>6</sub> | |||
|molar mass=786.35 | |||
|vendor=Sigma-Aldrich | |||
|product number=R2751 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=11103-72-3 | |||
|h statements=H302 | |||
:::'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>); Sigma R 2751, 1 g; M = 786.35 g·mol<sup>-1</sup>. | |h info=Harmful if swallowed | ||
}}<!--:::'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>); Sigma R 2751, 1 g; M = 786.35 g·mol<sup>-1</sup>.--> | |||
::::'''Preparation of 2 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''2-mL O2k-chamber''': | ::::'''Preparation of 2 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''2-mL O2k-chamber''': | ||
Revision as of 12:02, 10 June 2021
- high-resolution terminology - matching measurements at high-resolution
Ruthenium red
Description
Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca2+ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca2+-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].
Abbreviation: RR
Reference: MiPNet03.02, Wikipedia
Application in HRR
- Rut: Ruthenium red (Ammoniated ruthenium oxychloride; (NH3)5RuORu(NH3)4ORu(NH3)5Cl6), Sigma-Aldrich: R2751, store at RT, CAS: 11103-72-3, M = 786.35 g·mol-1
- Hazard statements: H302; Harmful if swallowed
- Rut: Ruthenium red (Ammoniated ruthenium oxychloride; (NH3)5RuORu(NH3)4ORu(NH3)5Cl6), Sigma-Aldrich: R2751, store at RT, CAS: 11103-72-3, M = 786.35 g·mol-1
- Preparation of 2 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 1.57 mg Ruthenium Red.
- Dissolve in 1 mL H2O.
- Divide into 0.1 mL aliquots.
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 1 µM.
- Preparation of 0.5 mM stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 50 µL of the 1 mM stock solution.
- Add 150 µL H2O.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 1 µM.
References
- Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
- Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
MitoPedia topics:
Inhibitor
