Ruthenium red: Difference between revisions

Revision as of 10:33, 11 September 2020

high-resolution terminology - matching measurements at high-resolution

Ruthenium red

Description

Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca²⁺ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca²⁺-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].

Abbreviation: Rut

Reference: MiPNet03.02, Wikipedia

Application in HRR

Rut: Ruthenium Red (technical grade; [(NH₃)₅RuORu(NH₃)₄ORu(NH₃)₅]Cl₆); Sigma R 2751, 1 g; FW = 786.35.

Preparation of 10 mM stock solution (dissolved in H₂O):

Weigh 7.86 mg Ruthenium Red.
Dissolve in 1 mL H₂O.
Divide into 0.2 mL aliquots.
Store at -20 °C.

References

Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.

MitoPedia topics: Inhibitor

@@ Line 4: / Line 4: @@
 |info=[[MiPNet03.02]], [http://en.wikipedia.org/wiki/Ruthenium_red Wikipedia]
 }}
-{{MitoPedia methods}}
+__TOC__
-{{MitoPedia topics
-|mitopedia topic=Inhibitor
-}}
 == Application in [[HRR]] ==
-'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>);   Sigma R 2751, 1 g; FW = 786.35.
+:::'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>);   Sigma R 2751, 1 g; FW = 786.35.
+::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
-'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
+::::# Weigh 7.86 mg Ruthenium Red.
+::::# Dissolve in 1 mL H<sub>2</sub>O.
-::1) Weigh 7.86 mg Ruthenium Red.
+::::# Divide into 0.2 mL aliquots.
-::2) Dissolve in 1 mL H<sub>2</sub>O.
+::::# Store at -20 °C.
-::3) Divide into 0.2 mL aliquots.
-::4) Store at -20 °C.
 == References ==
@@ Line 24: / Line 20: @@
 # Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
 # Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
+{{MitoPedia methods}}
+{{MitoPedia topics
+|mitopedia topic=Inhibitor
+}}