Rotenone: Difference between revisions
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''' | '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 1 ยตl using a 10 ยตl syringe (2 ml O2k-chamber). | ::* Titration volume: 1 ยตl using a 10 ยตl syringe (2 ml O2k-chamber). |
Revision as of 11:09, 13 May 2015
Description
Rotenone is an inhibitor of Complex I (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S(Rot).
Abbreviation: Rot
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Rot: Rotenone (C23H22O6); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (โฅ 95%).
Caution: Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!.
Preparation of 1 mM stock solution (dissolved in 99.9% ethanol):
- 1. Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%).
- 2. Divide into 100 ยตl aliquots (use dark glass vials).
- 3) Store frozen at -20 ยฐC.
O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 ยตl using a 10 ยตl syringe (2 ml O2k-chamber).
- Final concentration: 0.5 ยตM.
Inhibition of CI by rotenone
- Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384โ392. >>PMC2724837