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Rondot 2020 Thesis - Revision history
2024-03-29T01:16:32Z
Revision history for this page on the wiki
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Plangger Mario at 14:54, 17 June 2020
2020-06-17T14:54:39Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:54, 17 June 2020</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2020</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2020</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|journal=Master's Thesis</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|journal=Master's Thesis</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|abstract=The common eastern bumblebee queens (''Bombus impatiens'') endure cold winter months by entering a diapausalstate post-fertilization. During this overwintering period, these animals use stored energy reserves while maintaining a low metabolic rate. Bumblebees are thought to use primarily lipids to fuel this critical overwintering period,despite the fact <del style="font-weight: bold; text-decoration: none;">thatbee </del>mitochondria do not appear equipped to break down this metabolic fuel. For some insects, lipids stored in the fat body can be converted to the amino acid proline, and this metabolic fuel has recently been discovered to be readily oxidized by bumblebee workers.My research,therefore,<del style="font-weight: bold; text-decoration: none;">investigatesthe </del>role of proline during overwintering in bumblebee queens. <del style="font-weight: bold; text-decoration: none;">Usingcellular </del>respirometry, I determined the metabolic capacity of the muscle cells of queens to use various fuels,and if this capacity changes throughout overwintering. Surprisingly, the tested queens showed a much lower potential to oxidize proline than workers,and their capacity did not change during a four-month overwintering period.The metabolic properties of muscle tissue were further characterized using metabolic enzymes activity profile.These results further demonstrate the low potential for proline metabolism and the <del style="font-weight: bold; text-decoration: none;">limitationsof </del>bumblebee <del style="font-weight: bold; text-decoration: none;">queens’capacity </del>to oxidize lipids. Body composition <del style="font-weight: bold; text-decoration: none;">wasmeasured </del>to determine how the various energy stores (lipid, glycogen, protein) change during overwintering;however,no decrease in <del style="font-weight: bold; text-decoration: none;">concentrationwas </del>observed.Overall,this work <del style="font-weight: bold; text-decoration: none;">clarifiesthe </del>constraints of B. impatiens metabolism during overwintering.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|abstract=The common eastern bumblebee queens (''Bombus impatiens'') endure cold winter months by entering a diapausalstate post-fertilization. During this overwintering period, these animals use stored energy reserves while maintaining a low metabolic rate. Bumblebees are thought to use primarily lipids to fuel this critical overwintering period,despite the fact <ins style="font-weight: bold; text-decoration: none;">that bee </ins>mitochondria do not appear equipped to break down this metabolic fuel. For some insects, lipids stored in the fat body can be converted to the amino acid proline, and this metabolic fuel has recently been discovered to be readily oxidized by bumblebee workers. My research, therefore, <ins style="font-weight: bold; text-decoration: none;">investigates the </ins>role of proline during overwintering in bumblebee queens. <ins style="font-weight: bold; text-decoration: none;">Using cellular </ins>respirometry, I determined the metabolic capacity of the muscle cells of queens to use various fuels, and if this capacity changes throughout overwintering. Surprisingly, the tested queens showed a much lower potential to oxidize proline than workers, and their capacity did not change during a four-month overwintering period. The metabolic properties of muscle tissue were further characterized using metabolic enzymes activity profile. These results further demonstrate the low potential for proline metabolism and the <ins style="font-weight: bold; text-decoration: none;">limitations of </ins>bumblebee <ins style="font-weight: bold; text-decoration: none;">queens’ capacity </ins>to oxidize lipids. Body composition <ins style="font-weight: bold; text-decoration: none;">was measured </ins>to determine how the various energy stores (lipid, glycogen, protein) change during overwintering; however, no decrease in <ins style="font-weight: bold; text-decoration: none;">concentration was </ins>observed. Overall, this work <ins style="font-weight: bold; text-decoration: none;">clarifies the </ins>constraints of <ins style="font-weight: bold; text-decoration: none;">''</ins>B. impatiens<ins style="font-weight: bold; text-decoration: none;">'' </ins>metabolism during overwintering.</div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Rondot_2020_Thesis&diff=203886&oldid=prev
Plangger Mario at 14:51, 17 June 2020
2020-06-17T14:51:54Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:51, 17 June 2020</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|area=Respiration</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|area=Respiration</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k</div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Rondot_2020_Thesis&diff=203830&oldid=prev
Plangger Mario at 18:56, 16 June 2020
2020-06-16T18:56:37Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 18:56, 16 June 2020</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=Rondot A (2020) Fuels for winter: the role of proline in overwintering bumblebee queens (''Bombus impatiens''). Master's Thesis 41.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=Rondot A (2020) Fuels for winter: the role of proline in overwintering bumblebee queens (''Bombus impatiens''). Master's Thesis 41.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[https://ruor.uottawa.ca/bitstream/10393/40615/3/Rondot_Ariane_2020_thesis.pdf PDF]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[https://ruor.uottawa.ca/bitstream/10393/40615/3/Rondot_Ariane_2020_thesis.pdf PDF]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|authors=Rondot <del style="font-weight: bold; text-decoration: none;">A</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|authors=Rondot <ins style="font-weight: bold; text-decoration: none;">Ariane</ins></div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Rondot_2020_Thesis&diff=203829&oldid=prev
Plangger Mario: Created page with "{{Publication |title=Rondot A (2020) Fuels for winter: the role of proline in overwintering bumblebee queens (''Bombus impatiens''). Master's Thesis 41. |info=[https://ruor.uo..."
2020-06-16T18:56:24Z
<p>Created page with "{{Publication |title=Rondot A (2020) Fuels for winter: the role of proline in overwintering bumblebee queens (''Bombus impatiens''). Master's Thesis 41. |info=[https://ruor.uo..."</p>
<p><b>New page</b></p><div>{{Publication<br />
|title=Rondot A (2020) Fuels for winter: the role of proline in overwintering bumblebee queens (''Bombus impatiens''). Master's Thesis 41.<br />
|info=[https://ruor.uottawa.ca/bitstream/10393/40615/3/Rondot_Ariane_2020_thesis.pdf PDF]<br />
|authors=Rondot A<br />
|year=2020<br />
|journal=Master's Thesis<br />
|abstract=The common eastern bumblebee queens (''Bombus impatiens'') endure cold winter months by entering a diapausalstate post-fertilization. During this overwintering period, these animals use stored energy reserves while maintaining a low metabolic rate. Bumblebees are thought to use primarily lipids to fuel this critical overwintering period,despite the fact thatbee mitochondria do not appear equipped to break down this metabolic fuel. For some insects, lipids stored in the fat body can be converted to the amino acid proline, and this metabolic fuel has recently been discovered to be readily oxidized by bumblebee workers.My research,therefore,investigatesthe role of proline during overwintering in bumblebee queens. Usingcellular respirometry, I determined the metabolic capacity of the muscle cells of queens to use various fuels,and if this capacity changes throughout overwintering. Surprisingly, the tested queens showed a much lower potential to oxidize proline than workers,and their capacity did not change during a four-month overwintering period.The metabolic properties of muscle tissue were further characterized using metabolic enzymes activity profile.These results further demonstrate the low potential for proline metabolism and the limitationsof bumblebee queens’capacity to oxidize lipids. Body composition wasmeasured to determine how the various energy stores (lipid, glycogen, protein) change during overwintering;however,no decrease in concentrationwas observed.Overall,this work clarifiesthe constraints of B. impatiens metabolism during overwintering.<br />
|editor=[[Plangger M]]<br />
}}<br />
{{Labeling<br />
|area=Respiration<br />
|instruments=Oxygraph-2k<br />
|additional=2020-06<br />
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Plangger Mario