Roach 2022 Abstract Bioblast: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=2.4. Β«10+5Β» [[File:Roach T.jpg|left|100px|Roach Thomas]] <u>Roach Thomas</u>, Baur T, StΓΆggl W,Β Kranner I (2022) Retrograde signalling during high light stress involves reactive carbonyl / electrophile species. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 Β | |title=2.4. '''Β«10+5Β»''' [[File:Roach T.jpg|left|100px|Roach Thomas]] <u>Roach Thomas</u>, Baur T, StΓΆggl W,Β Kranner I (2022) Retrograde signalling during high light stress involves reactive carbonyl / electrophile species. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 Β | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors=Roach Thomas, Baur Theresa, Stoeggl Wolfgang, Kranner Ilse | |authors=Roach Thomas, Baur Theresa, Stoeggl Wolfgang, Kranner Ilse |
Revision as of 12:45, 19 June 2022
2.4. Β«10+5Β» Roach Thomas, Baur T, StΓΆggl W, Kranner I (2022) Retrograde signalling during high light stress involves reactive carbonyl / electrophile species. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 |
Link: Bioblast 2022: BEC Inaugural Conference
Roach Thomas, Baur Theresa, Stoeggl Wolfgang, Kranner Ilse (2022)
Event: Bioblast 2022
Singlet oxygen (1O2) is one of the most reactive of all reactive oxygen species (ROS), which is produced by chlorophyll during photosynthesis, and induces transcriptional changes in the nucleus, far from its reach. Here, using combined molecular, biochemical and physiological approaches, evidence is presented for how reactive carbonyl/electrophile species (RES) are involved in the acclimation of the model unicellular green alga Chlamydomonas reinhardtii to high light. Light stress led to an accumulation of RES, such as 2-propenal (acrolein) and 4-hydroxynonenal (HNE) [1,2], which are released when (1O2-derived lipid peroxides break down [3]. Treating cells with RES stimulated similar responses to high light, including higher tolerance to (1O2, similar patterns of protein carbonylation, and increased levels of glutathione [1,4], an important antioxidant involved in RES detoxification. A RNA seq. analysis revealed clear overlaps in gene regulation between RES-treated and high light-treated cells, which included an upregulation of many antioxidant enzymes and redox-related processes, as well as carotenoid and ubiquinone biosynthesis. However, most prominent was the overlap in down-regulated genes, whereby 70 % of the down-regulated genes under high light were also down-regulated by RES [1]. Moreover, the majority of these were shared when cells were treated with the photosenstizer Rose Bengal as an exogenous (1O2 source, confirming the specific role of (1O2 in RES signalling under high light stress. Finally, a comparison to differential gene expression in response to H2O2 revealed that half of the genes were also differentially expressed in the same direction after treatment with HNE, the only RES that increased upon treatment of cells with the same concentration of H2O2. Therefore, HNE is also a possible pathway for H2O2-mediated signalling.
- Roach T, Stoggl W, Baur T, Kranner I (2018) Distress and eustress of reactive electrophiles and relevance to light stress acclimation via stimulation of thiol/disulphide-based redox defences. https://doi.org/10.1016/j.freeradbiomed.2018.03.030
- Roach T, Na CS, StΓΆggl W, Krieger-Liszkay A (2020) The non-photochemical quenching protein LHCSR3 prevents oxygen-dependent photoinhibition in Chlamydomonas reinhardtii. https://doi.org/10.1093/jxb/eraa022
- Mano J, Biswas MS, Sugimoto K (2019) Reactive carbonyl species: A missing link in ROS signaling. https://doi.org/10.3390/plants8100391
- Roach T, Baur T, Stoggl W, Krieger-Liszkay A (2017) Chlamydomonas reinhardtii responding to high light: A role for 2-propenal (acrolein). https://doi.org/10.1111/ppl.12567
β’ Keywords: Photosynthesis, Chloroplast, light stress, Retrograde signalling, Reactive Oxygen Species
Affiliation
- Department of Botany, University of Innsbruck, Austria
Figures
List of abbreviations, terms and definitions - MitoPedia
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Event: A2