Difference between revisions of "Risiglione 2022 Life (Basel)"
(Created page with "{{Publication |title=Risiglione P, Cubisino SAM, Lipari CLR, De Pinto V, Messina A, Magrì A (2022) α-Synuclein A53T promotes mitochondrial proton gradient dissipation and de...") |
|||
Line 10: | Line 10: | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Genetic knockout;overexpression | ||
|diseases=Parkinson's | |diseases=Parkinson's | ||
|organism=Human | |||
|tissues=Neuroblastoma | |||
|preparations=Permeabilized cells, Intact cells | |||
|couplingstates=LEAK, ROUTINE, OXPHOS, ET | |||
|pathways=N, S, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2022-11 | |additional=2022-11 | ||
}} | }} |
Revision as of 14:06, 22 November 2022
Risiglione P, Cubisino SAM, Lipari CLR, De Pinto V, Messina A, Magrì A (2022) α-Synuclein A53T promotes mitochondrial proton gradient dissipation and depletion of the organelle respiratory reserve in a neuroblastoma cell line. https://doi.org/10.3390/life12060894 |
» Life (Basel) 12:894. PMID: 35743925 Open Access
Risiglione Pierpaolo, Cubisino Salvatore Antonio Maria, Lipari Cristiana Lucia Rita, De Pinto Vito, Messina Angela, Magri Andrea (2022) Life (Basel)
Abstract: α-synuclein (αSyn) is a small neuronal protein whose accumulation correlates with Parkinson's disease. αSyn A53T mutant impairs mitochondrial functions by affecting substrate import within the organelle, activity of complex I and the maximal respiratory capacity. However, the precise mechanism initiating the bioenergetic dysfunction is not clearly understood yet. By overexpressing αSyn A53T in SH-SY5Y cells, we investigated the specific changes in the mitochondrial respiratory profile using High-Resolution Respirometry. We found that αSyn A53T increases dissipative fluxes across the intermembrane mitochondrial space: this does not compromise the oxygen flows devoted to ATP production while it reduces the bioenergetic excess capacity of mitochondria, providing a possible explanation of the increased cell susceptibility observed in the presence of further stress stimuli. • Keywords: Parkinson’s disease, High-resolution respirometry, Mitochondrial dysfunction, αSyn • Bioblast editor: Plangger M
Labels: MiParea: Respiration, Genetic knockout;overexpression
Pathology: Parkinson's
Organism: Human Tissue;cell: Neuroblastoma Preparation: Permeabilized cells, Intact cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
2022-11