Difference between revisions of "Reverse electron flow from CII to CI"
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|description=Reverse electron flow from CII to CI stimulates production of [[ROS]] when mitochondria are incubated with succinate without rotenone in the LEAK state. Depolarisation of | |description=Reverse electron flow from CII to CI stimulates production of [[ROS]] when mitochondria are incubated with succinate without rotenone in the LEAK state at a high [[mt-membrane potential]]. Depolarisation of the mt-membrane potential (''e.g.'' after ADP addition to stimulate OXPHOS) leads to inhibition of RET and therefore, decrease of RET-initiated ROS production. RET can be also measured when mitochondria are respiring using [[Glycerophosphate |Gp]] without rotenone in the [[LEAK respiration|LEAK]] state. Addition of I<sub>Q</sub>-side inhibitors (ubiquinone-binding side of CI) of [[Complex I |CI]] usually block RET. The following SUIT protocols allow you to measure RET-initiated H<sub>2</sub>O<sub>2</sub> flux in [[mitochondrial preparations]]: [[SUIT-009]] and [[SUIT-026]]. | ||
|info= | |info= | ||
* <div>[ | * <div>[https://wiki.oroboros.at/index.php/Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]</div> | ||
* [https://wiki.oroboros.at/index.php/Komlodi_2018_J_Bioenerg_Biomembr Komlodi_2018_J_Bioenerg_Biomembr] | * [https://wiki.oroboros.at/index.php/Komlodi_2018_J_Bioenerg_Biomembr Komlodi_2018_J_Bioenerg_Biomembr] | ||
* [https://wiki.oroboros.at/index.php/Makrecka-Kuka_2015_Biomolecules Makrecka-Kuka_2015_Biomolecules] | |||
* [https://wiki.oroboros.at/index.php/Tretter_2012_Free_Radic_Biol_Med Tretter_2012_Free_Radic_Biol_Med] | * [https://wiki.oroboros.at/index.php/Tretter_2012_Free_Radic_Biol_Med Tretter_2012_Free_Radic_Biol_Med] | ||
* [https://onlinelibrary.wiley.com/doi/full/10.1111/j.1471-4159.2006.04223.x Tretter_2006_J_Neurochem] | * [https://onlinelibrary.wiley.com/doi/full/10.1111/j.1471-4159.2006.04223.x Tretter_2006_J_Neurochem] | ||
* [[ | * [[Amplex UltraRed]] | ||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
Latest revision as of 12:20, 25 November 2020
- high-resolution terminology - matching measurements at high-resolution
Reverse electron flow from CII to CI
Description
Reverse electron flow from CII to CI stimulates production of ROS when mitochondria are incubated with succinate without rotenone in the LEAK state at a high mt-membrane potential. Depolarisation of the mt-membrane potential (e.g. after ADP addition to stimulate OXPHOS) leads to inhibition of RET and therefore, decrease of RET-initiated ROS production. RET can be also measured when mitochondria are respiring using Gp without rotenone in the LEAK state. Addition of IQ-side inhibitors (ubiquinone-binding side of CI) of CI usually block RET. The following SUIT protocols allow you to measure RET-initiated H2O2 flux in mitochondrial preparations: SUIT-009 and SUIT-026.
Abbreviation: RET
Reference:
MitoPedia topics: Enzyme
