Difference between revisions of "Reverse electron flow from CII to CI"
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|description=Reverse electron flow from CII to CI stimulates production of [[ROS]] when mitochondria are incubated with succinate without rotenone. | |description=Reverse electron flow from CII to CI stimulates production of [[ROS]] when mitochondria are incubated with succinate without rotenone in the LEAK state. Depolarisation of mitochondrial membrane potential (e.g. after ADP addition to stimulate OXPHOS) leads to inhibition of RET and therefore, decrease of RET-initiated ROS production. RET can be also measured when mitochondria are respiring on [[Glycerophosphate |Gp]] without rotenone in the [[LEAK]] state. Addition of I<sub>Q</sub>-side inhibitors (ubiquinone-binding side of CI) of [[Complex I |CI]] usually block RET. | ||
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways] | |info= | ||
* <div>[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]</div> | |||
* [https://wiki.oroboros.at/index.php/Komlodi_2018_J_Bioenerg_Biomembr Komlodi_2018_J_Bioenerg_Biomembr] | |||
* [https://wiki.oroboros.at/index.php/Tretter_2012_Free_Radic_Biol_Med Tretter_2012_Free_Radic_Biol_Med] | |||
* [https://onlinelibrary.wiley.com/doi/full/10.1111/j.1471-4159.2006.04223.x Tretter_2006_J_Neurochem] | |||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
Revision as of 10:39, 27 July 2020
- high-resolution terminology - matching measurements at high-resolution
Reverse electron flow from CII to CI
Description
Reverse electron flow from CII to CI stimulates production of ROS when mitochondria are incubated with succinate without rotenone in the LEAK state. Depolarisation of mitochondrial membrane potential (e.g. after ADP addition to stimulate OXPHOS) leads to inhibition of RET and therefore, decrease of RET-initiated ROS production. RET can be also measured when mitochondria are respiring on Gp without rotenone in the LEAK state. Addition of IQ-side inhibitors (ubiquinone-binding side of CI) of CI usually block RET.
Reference:
MitoPedia topics: Enzyme
