Difference between revisions of "Respiratory state"
From Bioblast
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|mitopedia method=Respirometry, Spectrophotometry | |mitopedia method=Respirometry, Spectrophotometry | ||
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[[Image:P.jpg|right|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|right|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|right|link=ETS capacity|ETS]] [[Image:L.jpg|right|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|right|link=Residual oxygen consumption|ROX]] | [[Image:P.jpg|right|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|right|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|right|link=ETS capacity|ETS]] [[Image:L.jpg|right|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|right|link=Residual oxygen consumption|ROX]] | ||
== Coupling control states == | == Coupling control states == | ||
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== MitoPedia: Respiratory states == | == MitoPedia: Respiratory states == | ||
:::: | ::::Β» See the complete '''[[MitoPedia: Respiratory states]]''' | ||
:::: | ::::Β» [[MitoPedia: SUIT]] |
Revision as of 09:18, 15 May 2016
Description
Respiratory states of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling control state and the substrate control state.
Reference: Gnaiger 2014 MitoPathways
MitoPedia concepts:
MiP concept,
Respiratory state,
SUIT concept
MitoPedia methods:
Respirometry,
Spectrophotometry
Coupling control states
- Coupling states and CCR of mitochondrial preparations:
- Coupling states of intact cells:
Substrate control states
- Substrate control states, SCR, are defined by substrate type (at saturating concentration) as pathway control states:
- Intact cells: endogenous, exogenous substrate control
- Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with fatty acid oxidation, alpha-glycerophosphate, (Complex I-linked substrate state, Complex II-linked substrate state, etc.), or substrate combinations applied for reconstitution of TCA cycle function (e.g. Complex I&II-linked substrate state, etc.).
- Control by substrate concentration: Kinetic control states:
- Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
- Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.
Classical respiratory states
- Chance and Williams (1955):
- Derived respiratory states:
- Thermodynamics of irreversible processes:
MitoPedia: Respiratory states
- Β» See the complete MitoPedia: Respiratory states
- Β» MitoPedia: SUIT