Renner 2003 Biochim Biophys Acta

Has title::Renner K, Amberger A, Konwalinka G, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642: 115-123.

» [[Has info::PMID: 12972300]]

Was written by::Renner K, Was written by::Amberger A, Was written by::Konwalinka G, Was written by::Gnaiger E (Was published in year::2003) Was published in journal::Biochim Biophys Acta

Abstract: [[has abstract::Mitochondrial damage with release of cytochrome c is implicated in cell death signalling pathways. To examine mitochondrial function in apoptotic cells, we applied high-resolution respirometry to human leukemia cells arrested in the G1- and S-phase by exposure to the glucocorticoid dexamethasone and nucleotide analogue gemcitabine. At 30 % apoptosis, opposite effects were observed on respiratory capacity (71 % and 131 % of controls, respectively). These changes correlated with alterations in cell size, cytosolic, and mitochondrial marker enzymes. Mitochondrial ATP production and membrane potential were maintained in all treatments, as deduced from high respiratory uncoupling control ratios. Bcl-2 over-expression did not prevent apoptosis after gemcitabine-treatment, but protected dexamethasone-treated cells from apoptosis, without fully preventing the decline of respiration and cell size.

These results, therefore, provide conclusive evidence that alterations in respiratory capacity and enzyme activities per cell are mainly caused by opposite changes in cell size, occurring upon cell cycle arrest triggered by dexamethasone and gemcitabine in the early phase of apoptosis.]] • Keywords: has publicationkeywords::Apoptosis, has publicationkeywords::Dexamethasone, has publicationkeywords::Gemcitabine, has publicationkeywords::Mitochondrial respiratory control, has publicationkeywords::Cell cycle

• O2k-Network Lab: Was published by MiPNetLab::AT_Innsbruck_Gnaiger E, Was published by MiPNetLab::AT Innsbruck MitoCom

Labels: MiParea: MiP area::Respiration, MiP area::mt-Biogenesis; mt-density, MiP area::Genetic knockout; overexpression, MiP area::Pharmacology; toxicology  Pathology: Diseases::Cancer 

Organism: Organism::Human  Tissue;cell: tissue and cell::Blood cells  Preparation: Preparation::Intact cells, Preparation::Permeabilized cells, Preparation::Oxidase; Biochemical Oxidation, Preparation::Enzyme 

Coupling state: Coupling states::OXPHOS, Coupling states::ETS 

HRR: Instrument and method::Oxygraph-2k 

• Referred to in Gnaiger_2012_MitoPathways, Chapter 1.