Ranganathan 2020 Mitochondrion

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Ranganathan A, Owiredu S, Jang DH, Eckmann DM (2020) Prophylaxis of mitochondrial dysfunction caused by cellular decompression from hyperbaric exposure . Mitochondrion 52:8-19.

» PMID: 32045716

Ranganathan A, Owiredu S, Jang DH, Eckmann DM (2020) Mitochondrion

Abstract: Mitochondrial dysfunction occurring in response to cellular perturbations can include altered mitochondrial motility and bioenergetic function having intracellular heterogeneity. Exogenous mitochondrial directed therapy may correct these dysfunctions. Using in vitro approaches, we find that cell perturbations induced by rapid decompression from hyperbaric conditions with specific gas exposures has differential effects on mitochondrial motility, inner membrane potential, cellular respiration, reactive oxygen species production, impaired maintenance of cell shape and altered intracellular distribution of bioenergetic capacity in perinuclear and cell peripheral domains. Addition of a first-generation cell-permeable succinate prodrug to support mitochondrial function has positive overall effects in blunting the resultant bioenergy responses. Our results with this model of perturbed cell function induced by rapid decompression indicate that alterations in bioenergetic state are partitioned within the cell, as directly assessed by a combination of mitochondrial respiration and dynamics measurements. Reductions in the observed level of dysfunction produced can be achieved with application of the cell-permeable succinate prodrug.

Keywords: Bioenergetics, Decompression, Intermembrane potential, Microscopy, Mitochondria, Motility, Perinuclear, Respiration Bioblast editor: Plangger M O2k-Network Lab: US PA Philadelphia Jang DH

Labels: MiParea: Respiration 

HRR: Oxygraph-2k