ROUTINE respiration: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=''R'' | |abbr=''R'' | ||
|description=In the intact cell, '''ROUTINE respiration''' in the physiological coupling [[State R|state ''R'']], is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic [[uncoupling]] and pathological [[dyscoupling]]).ย ''R'' and growth of cells is supported by exogenous substrates in culture media. In media without energy substrates, ''R'' depends on endogenous substrates. ''R'' cannot be measured in [[permeabilized cells]] or [[isolated mitochondria]]. ''R'' is corrected for [[residual oxygen consumption]] (ROX), whereas ''R''ยด is the uncorrected apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX. | |description=[[File:R.jpg]] | ||
In the intact cell, '''ROUTINE respiration''' in the physiological coupling [[State R|state ''R'']], is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic [[uncoupling]] and pathological [[dyscoupling]]).ย ''R'' and growth of cells is supported by exogenous substrates in culture media. In media without energy substrates, ''R'' depends on endogenous substrates. ''R'' cannot be measured in [[permeabilized cells]] or [[isolated mitochondria]]. ''R'' is corrected for [[residual oxygen consumption]] (ROX), whereas ''R''ยด is the uncorrected apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX. | |||
|info=[[Gnaiger 2012 MitoPathways]], [[Gnaiger_2008_POS]] | |info=[[Gnaiger 2012 MitoPathways]], [[Gnaiger_2008_POS]] | ||
|type=Respiration | |type=Respiration | ||
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'''Basal respiration''' or "basal metabolic rate" is frequently used as [[LEAK respiration]]. Importantly, we have to make a clear distinction between (oligomycin-inhibited) LEAK respiration and physiologically controlled [[ROUTINE respiration]], none of which conform to the physiological definition of [[basal respiration]]. | '''Basal respiration''' or "basal metabolic rate" is frequently used as [[LEAK respiration]]. Importantly, we have to make a clear distinction between (oligomycin-inhibited) LEAK respiration and physiologically controlled [[ROUTINE respiration]], none of which conform to the physiological definition of [[basal respiration]]. | ||
== | == Related terms in Bioblast == | ||
* [[OXPHOS capacity]] | * [[OXPHOS capacity]] |
Revision as of 10:44, 21 June 2014
Description
In the intact cell, ROUTINE respiration in the physiological coupling state R, is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation (intrinsic uncoupling and pathological dyscoupling). R and growth of cells is supported by exogenous substrates in culture media. In media without energy substrates, R depends on endogenous substrates. R cannot be measured in permeabilized cells or isolated mitochondria. R is corrected for residual oxygen consumption (ROX), whereas Rยด is the uncorrected apparent ROUTINE respiration or total cellular oxygen consumption of cells including ROX.
Abbreviation: R
Reference: Gnaiger 2012 MitoPathways, Gnaiger_2008_POS
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Why not 'basal respiration', and what about 'State 3.5'?
Basal respiration or "basal metabolic rate" is frequently used as LEAK respiration. Importantly, we have to make a clear distinction between (oligomycin-inhibited) LEAK respiration and physiologically controlled ROUTINE respiration, none of which conform to the physiological definition of basal respiration.