- high-resolution terminology - matching measurements at high-resolution
R-L net ROUTINE capacity
Description
The free ROUTINE-activity is ROUTINE respiration corrected for LEAK respiration, R-L. R-L is the respiratory activity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in living cells, therefore, is partitioned into the free ROUTINE-activity, R-L, strictly coupled to phosphorylation, P», and nonphosphorylating LEAK respiration, LR, compensating for proton leaks, slip and cation cycling: R = R-L+LR. It is frequently assumed that LEAK respiration, L, as measured in the LEAK state, overestimates the LEAK component of respiration, LR, as measured in the ROUTINE-state, particularly if the protonmotive force is not adjusted to equivalent levels in L and LR. However, if the LEAK component increases with enzyme turnover during R, the low enzyme turnover during L may counteract the effect of the higher pmF.
Abbreviation: R-L
Reference: Gnaiger 2020 MitoPathways
Communicated by Gnaiger E (2014-09-21) last update 2020-11-08.
Compare
- Free OXPHOS-capacity (mt-preparations)
- Free ET-capacity
MitoPedia concepts:
Respiratory state,
Recommended
MitoPedia topics:
EAGLE
