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Difference between revisions of "R-L net ROUTINE capacity"

From Bioblast
m (Gnaiger Erich moved page Free ROUTINE activity to Free ROUTINE-activity)
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{{MitoPedia
{{MitoPedia
|abbr=''β‰ˆR''
|abbr=''R-L''
|description=[[Image:R-L.jpg|50 px|Free ROUTINE activity]] The '''free ROUTINE activity''', ''β‰ˆR'', is [[ROUTINE respiration]] corrected for [[LEAK respiration]], ''β‰ˆR'' = ''R-L''. ''β‰ˆR'' is the respiratory activity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in intact cells, therefore, is partitioned into the free ROUTINE activity, ''β‰ˆR'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>R</sub>'', compensating for proton leaks, slip and cation cycling: ''R'' = ''β‰ˆR''+''L<sub>R</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>R</sub>'', as measured in the ROUTINE state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>R</sub>''. However, if the LEAK component increases with enzyme turnover during ''R'', the low enzyme turnover during ''L'' may counteract the effect of the higher Ξ”''p''<sub>mt</sub>.
|description=[[Image:R-L.jpg|50 px|Free ROUTINE-activity]] The '''free ROUTINE-activity''' is [[ROUTINE respiration]] corrected for [[LEAK respiration]], ''R-L''. ''R-L'' is the respiratory activity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in living cells, therefore, is partitioned into the free ROUTINE-activity, ''R-L'', strictly coupled to phosphorylation, ''PΒ»'', and nonphosphorylating LEAK respiration, ''L<sub>R</sub>'', compensating for proton leaks, slip and cation cycling: ''R'' = ''R-L''+''L<sub>R</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>R</sub>'', as measured in the ROUTINE-state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>R</sub>''. However, if the LEAK component increases with enzyme turnover during ''R'', the low enzyme turnover during ''L'' may counteract the effect of the higher ''pmF''.
|info=[[Gnaiger 2014 MitoPathways]]
|info=[[Gnaiger 2020 MitoPathways]]
}}
}}
Communicated by [[Gnaiger E]] (2014-09-21) last update 2020-11-08.
[[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger 2020 MitoPathways |The Blue Book 2014]]]]
== Compare ==
* [[Free OXPHOS-capacity]] (mt-preparations)
* [[Free ET-capacity]]
{{MitoPedia concepts
{{MitoPedia concepts
|mitopedia concept=Respiratory state, Recommended
|mitopedia concept=Respiratory state, Recommended
}}
}}
{{MitoPedia methods}}
{{MitoPedia O2k and high-resolution respirometry}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=EAGLE
|mitopedia topic=EAGLE
}}
}}
Communicated by [[Gnaiger E]] 2014-09-21, edited 2015-01-19.
[[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]]
== Compare ==
* [[Free OXPHOS capacity]] (mt-preparations)
* [[Free ET capacity]]

Revision as of 10:52, 8 November 2020


high-resolution terminology - matching measurements at high-resolution


R-L net ROUTINE capacity

Description

Free ROUTINE-activity The free ROUTINE-activity is ROUTINE respiration corrected for LEAK respiration, R-L. R-L is the respiratory activity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in living cells, therefore, is partitioned into the free ROUTINE-activity, R-L, strictly coupled to phosphorylation, PΒ», and nonphosphorylating LEAK respiration, LR, compensating for proton leaks, slip and cation cycling: R = R-L+LR. It is frequently assumed that LEAK respiration, L, as measured in the LEAK state, overestimates the LEAK component of respiration, LR, as measured in the ROUTINE-state, particularly if the protonmotive force is not adjusted to equivalent levels in L and LR. However, if the LEAK component increases with enzyme turnover during R, the low enzyme turnover during L may counteract the effect of the higher pmF.

Abbreviation: R-L

Reference: Gnaiger 2020 MitoPathways

Communicated by Gnaiger E (2014-09-21) last update 2020-11-08.

Compare


MitoPedia concepts: Respiratory state, Recommended 


MitoPedia topics: EAGLE