Permeabilized tissue or cells: Difference between revisions
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|abbr=pti, pce | |abbr=pti, pce | ||
|description='''Permeabilized tissue''' (pti, see also [[permeabilized muscle fibres]], pfi) or cells (pce) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or [[permeabilization of plasma membrane|chemically]], for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes. | |description='''Permeabilized tissue''' ([[Permeabilized tissue|pti]], see also [[permeabilized muscle fibres]], pfi) or cells ([[Permeabilized cells|pce]]) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or [[permeabilization of plasma membrane|chemically]], for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes. | ||
'''Permeabilized cells''' (pce) are, therefore, not any longer viable or [[intact cells]] (ce), since the intactness of cells implies the intactness of the plasma membrane. Any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells. | '''Permeabilized cells''' (pce) are, therefore, not any longer viable or [[intact cells]] (ce), since the intactness of cells implies the intactness of the plasma membrane. Any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells. |
Revision as of 15:53, 20 March 2019
- high-resolution terminology - matching measurements at high-resolution
Permeabilized tissue or cells
Description
Permeabilized tissue (pti, see also permeabilized muscle fibres, pfi) or cells (pce) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically, for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
Permeabilized cells (pce) are, therefore, not any longer viable or intact cells (ce), since the intactness of cells implies the intactness of the plasma membrane. Any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells.
Abbreviation: pti, pce
Reference: MiPNet11.05; O2k-Publications: Permeabilized cells
MitoPedia topics:
Sample preparation