Difference between revisions of "Permeabilized tissue"
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|info=[[MiPNet11.05]]; [[O2k-Publications: Permeabilized tissue]] | |info=[[MiPNet11.05]]; [[O2k-Publications: Permeabilized tissue]] | ||
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== Experimental SOP == | == Experimental SOP == | ||
'''Addition of sample to the respirometer chamber:''' For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again. | '''Addition of sample to the respirometer chamber:''' For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again. | ||
== References == | |||
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|mitopedia topic=Sample preparation | |||
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Latest revision as of 17:08, 26 May 2020
- high-resolution terminology - matching measurements at high-resolution
Permeabilized tissue
Description
Permeabilized tissue (pti, see also permeabilized muscle fibers, pfi) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically (e.g., with saponin), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
Abbreviation: pti
Reference: MiPNet11.05; O2k-Publications: Permeabilized tissue
Experimental SOP
Addition of sample to the respirometer chamber: For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again.
References
| Bioblast link | Reference | Year |
|---|---|---|
| BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
MitoPedia topics:
Sample preparation
