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Difference between revisions of "Permeabilized tissue"

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== Experimental SOP ==
Addition of sample to the respirometer chamber: For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again.

Revision as of 16:55, 18 March 2019


high-resolution terminology - matching measurements at high-resolution


Permeabilized tissue

Description

Permeabilized tissue (pti, see also permeabilized muscle fibres, pfi) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically (e.g., with saponin), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.

Abbreviation: pti

Reference: MiPNet11.05; O2k-Publications: Permeabilized tissue


MitoPedia topics: Sample preparation 


Experimental SOP

Addition of sample to the respirometer chamber: For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again.