Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Pathway control ratio"

From Bioblast
Line 1: Line 1:
{{MitoPedia
{{MitoPedia
|abbr=''SCR''
|abbr=''SCR''
|description='''Substrate control ratios''', ''SCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[coupling state]]. Whereas there are only three well-defined coupling states of mitochondrial respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]), numerous [[substrate state]]s are possible. In contrast, the [[reference state]], ''Z'', is strictly defined for [[flux control capacity|flux control capacities]]
|description='''Substrate control ratios''', ''SCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[coupling state]]. Whereas there are only three well-defined coupling states of mitochondrial respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]), numerous [[substrate state]]s are possible. In contrast, the [[reference state]], ''Z'', is strictly defined for [[flux control capacity|flux control capacities]].


Careful selection of the reference state, ''J''<sub>ref</sub>, is required, for which some guidelines may be provided without the possibility to formulate general rules. ''FCR'' are best defined by taking ''J''<sub>ref</sub> as the maximum flux (e.g. [[Additive effect of convergent CI+II electron flow|CI+II]]<sub>''[[ETS|E]]''</sub>), such that flux in various other respiratory states, ''J<sub>i</sub>'', smaller or equal to ''J''<sub>ref</sub>. However, this is not generally possible with ''SCR''. For instance, the CI/CII substrate control ratio (at constant coupling state) may be larger or smaller than 1.0, depending on the mitochondrial source and various mitochondrial injuries. The CII-respiration may be selected preferentially as ''J''<sub>ref</sub>, if mitochondria with variable CI-related injuries are studied. In contrast, the [[reference state]], ''Z'' is strictly defined for [[flux control capacity|flux control capacities]].
Careful selection of the reference state, ''J''<sub>ref</sub>, is required, for which some guidelines may be provided without the possibility to formulate general rules. ''FCR'' are best defined by taking ''J''<sub>ref</sub> as the maximum flux (e.g. [[Additive effect of convergent CI+II electron flow|CI+II]]<sub>''[[ETS|E]]''</sub>), such that flux in various other respiratory states, ''J<sub>i</sub>'', smaller or equal to ''J''<sub>ref</sub>. However, this is not generally possible with ''SCR''. For instance, the CI/CII substrate control ratio (at constant coupling state) may be larger or smaller than 1.0, depending on the mitochondrial source and various mitochondrial injuries. The CII-respiration may be selected preferentially as ''J''<sub>ref</sub>, if mitochondria with variable CI-related injuries are studied.
|info=[[Gnaiger 2009 Int J Biochem Cell Biol]]; [[List of respiratory states]]
|info=[[Gnaiger 2009 Int J Biochem Cell Biol]]; [[List of respiratory states]]
}}
}}

Revision as of 13:54, 5 August 2013


high-resolution terminology - matching measurements at high-resolution


Pathway control ratio

Description

Substrate control ratios, SCR, are flux control ratios, FCR, at a constant mitochondrial coupling state. Whereas there are only three well-defined coupling states of mitochondrial respiration, L, P, E (LEAK, OXPHOS, ETS), numerous substrate states are possible. In contrast, the reference state, Z, is strictly defined for flux control capacities.

Careful selection of the reference state, Jref, is required, for which some guidelines may be provided without the possibility to formulate general rules. FCR are best defined by taking Jref as the maximum flux (e.g. CI+IIE), such that flux in various other respiratory states, Ji, smaller or equal to Jref. However, this is not generally possible with SCR. For instance, the CI/CII substrate control ratio (at constant coupling state) may be larger or smaller than 1.0, depending on the mitochondrial source and various mitochondrial injuries. The CII-respiration may be selected preferentially as Jref, if mitochondria with variable CI-related injuries are studied.

Abbreviation: SCR

Reference: Gnaiger 2009 Int J Biochem Cell Biol; List of respiratory states


MitoPedia methods: Respirometry 


MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.